Advancing Age Has Differential Effects on DNA Damage, Chromatin Integrity, Gene Mutations, and Aneuploidies in Sperm

Abstract

Numerous trials have concluded that laparoscopic hysterectomy, compared with total abdominal hysterectomy, causes less postoperative pain and shortens the hospital stay. Many view this approach as being more cost-effective, but a recent large, randomized trial demonstrated more major complications after the laparoscopic procedure. The present study compared the length of time in the hospital, time to convalescence, and long-term patient satisfaction in 47 consecutive women who were to have supravaginal hysterectomy. Twenty-three of them were randomized to undergo abdominal supravaginal hysterectomy (ASH) and 24 laparoscopic supravaginal hysterectomy (LSH). Pre-and postoperative procedures were comparable in the 2 groups, and there were no significant differences in demographic features or physical characteristics.The postoperative hospital stay was comparable after ASH and LSH. Operating times were significantly longer with LSH, but estimated blood loss was greater in the ASH group. No intraoperative complications occurred in either group, and no patient was transfused. Self-rated pain 6 hours postoperatively was less in women having LSH. Follow up at 6 weeks showed that patients having ASH required approximately 10 more disability days than those in the LSH group. There were no differences in the number of days analgesics were required or the time needed to resume normal activities. At 6 months, 87% of women having ASH and 91% of those in the LSH group were satisfied or very satisfied with the overall results. More than 90% of women in both groups would recommend their procedure to others.When using a multimodal intervention program of postoperative care, the choice between ASH or LSH may be less important than is generally believed with regard to postoperative time in the hospital and long-term patient satisfaction. EDITORIAL COMMENT(What is with the idea of supracervical hysterectomy? There may be some patients who think that preservation of the cervix has less risk of interfering with sexual function or producing urinary incontinence. However, several prospective, randomized trials from the United States, Holland, England, and Denmark have shown that these ideas are not true-preservation of the cervix does not help with sexual, bladder, or bowel function.Learman et al reported on a relatively small, prospectively randomized series of 135 women from 4 U.S. academic medical centers who were followed for 2 years after hysterectomy (Obstet Gynecol 2003;102:453). They found no statistically significant difference in rate of complication, length of hospital stay, or clinical outcomes specifically related to symptoms of bladder or bowel dysfunction and pelvic and back pain between the total and supracervical abdominal hysterectomy GYNECOLOGY Volume 61, Number 10 OBSTETRICAL AND GYNECOLOGICAL SURVEY ABSTRACT Endometriosis is among the most important causes of chronic pelvic pain (CPP) in women of reproductive age; it reportedly is present in as many as one third of women having diagnostic laparoscopy for pain. This ...

Detection of DNA fragmentation and meiotic segregation in human with isolated teratozoospermia

“…In the present study, no correlation was found between the age of our patients and the levels of DNA fragmentation. This is in agreement with the results of Dakouane et al [38] who found no difference in DNA fragmentation rate according to age among 212 men aged between 25 and 70 years, but in disagreement with the results of others studies [39,40] who had found a significant correlation between DNA fragmentation and age of the patients. In addition, a positive correlation was found between the rate of sperm DNA fragmentation and the rate of sperm chromosomal aberration.…”

Frequency of human sperm carrying structural aberrations of chromosome 1 increases with advancing age

“…This is in agreement with the results of another study of the same population that used a sperm FISH assay specific for numerical chromosomal abnormalities, i.e., aneuploidy and diploidy (77). Previous results from other FISH studies provided ambiguous evidence for an association of men's age and autosomal aneuploidies in sperm (37,(77)(78)(79)(80)(81).…”

Evaluation of semen quality in roosters of different age during hot climatic condition

“…Age of human subjects had been shown to have strong effect on sperm chromatin defects. Aged men had significantly higher DNA fragmentation index; the damage was found to be doubled between 20 and 60 years of age (Wyrobek et al, 2006). In our study apart from the 65 week age roosters the 23 week age roosters were also found to have higher DNA fragmentation.…”