Advancing post-anoxic denitrification for biological nutrient removal

Winkler, Matt; Coats, Erik R.; Brinkman, Cynthia K.

doi:10.1016/j.watres.2011.09.006

Cited by 100 publications

(72 citation statements)

References 43 publications

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“…Figure 7 also shows the relationships between PHA or glycogen concentrations and endogenous denitrification rates. Although Winkler et al (2011) have reported that a linear relationship exists between the endogenous denitrification rate and storage glycogen levels in the biomass, no correlation was seen between them in this study. This is probably because stored glycogen was not being consumed as an electron donor in this study (Fig.…”

Section: Relationship Between Denitrification Rates and Endogenous Cacontrasting

confidence: 88%

“…It has been reported that PHB can be used as an endogenous carbon source for denitrification when all external carbon sources are depleted (Bernat et al, 2008;Wu et al, 2010), while some work suggest that its ability to support denitrification is very limited (Qin et al, 2005). Moreover, post-anoxic denitrification in EBPR system is thought to be driven predominantly by glycogen as electron donor, although PHA can also be used Winkler et al, 2011). However, few studies have evaluated the possible effects of the internally stored carbon sources on the rates of endogenous denitrification.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Endogenous Carbon Source on Biological Denitrification Rate

Mikami

Nittami

Kurisu

2013

J. of Wat. ^|^ Envir. Tech.

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Post-anoxic denifitrication process can remove nitrogen from wastewater at a lower cost than pre-anoxic denitrification process since the nitrified water is not circulated during this process unless exogenous carbon addition is required. However, the denitrification rate is generally lower than that of a pre-anoxic process because endogenous carbon sources within the denitrifying bacteria are used as electron donors. The objective of this study was to develop a better understanding of this endogenous denitrification rate. A sequencing batch reactor fed with propionate was operated under variable anaerobic-aerobic conditions for 26 days to allow the biomass to accumulate polyhydroxyalkanoate (PHA) and glycogen stores. Sludge samples were collected from the reactor periodically, and nitrate removal rates were measured in the absence of any external carbon sources. Nitrate reduction rates ranged from 1.20 to 2.74 mgN/gMLSS/h. Meanwhile, PHA consumption (1.04 -8.01 mgC/gMLSS/h) and glycogen synthesis (0 -4.74 mgC/gMLSS/h) and consumption (0 -1.54 mgC/gMLSS/h) were observed. The PHA consumption correlated positively with the nitrate reduction rate. Furthermore, glycogen consumption seemed to reduce both PHA consumption and nitrate reduction.

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Section: Relationship Between Denitrification Rates and Endogenous Cacontrasting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Effect of Endogenous Carbon Source on Biological Denitrification Rate

Mikami

Nittami

Kurisu

2013

J. of Wat. ^|^ Envir. Tech.

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“…and Nitrospira were amplified using 16S rDNA sequences. qPCR settings were in accordance with Winkler et al (2011). AOB, Nitrobacter , and Nitrospira abundance relative to eubacteria was estimated using the mean efficiencies for each primer set and the Cq values for the individual samples, assuming average 16S rDNA gene copy numbers of 4.1 for eubacteria, 2.5 for AOB (Leininger et al 2006), and 1.0 for both Nitrobacter and Nitrospira (McIlroy et al 2015).…”

Section: Methodsmentioning

confidence: 99%

Polyhydroxyalkanoate synthesis by mixed microbial consortia cultured on fermented dairy manure: Effect of aeration on process rates/yields and the associated microbial ecology

2016

Self Cite

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Polyhydroxyalkanoates (PHAs) are biodegradable polymers that can substitute for petroleum-based plastics in a variety of applications. One avenue to commercial PHA production involves coupling waste-based synthesis with the use of mixed microbial consortia (MMC). In this regard, production requires maximizing the enrichment of a MMC capable of feast-famine PHA synthesis, with the metabolic response induced through imposition of aerobic-dynamic feeding (ADF) conditions. However, the concept of PHA production in complex matrices remains unrefined; process operational improvements are needed, along with an enhanced understanding of the MMC. Research presented herein investigated the effect of aeration on feast-famine PHA synthesis, with four independent aeration state systems studied; MMC were fed volatile fatty acid (VFA)-rich fermented dairy manure. Regardless of the aeration state, all MMC exhibited a feast-famine response based on observed carbon cycling. Moreover, there was no statistical difference in PHA synthesis rates, with qPHA ranging from 0.10–0.19 CmmolPHA gVSS−1 min−1; VFA uptake rates exhibited similar statistical indifferences. PHA production assessments on the enriched MMC resulted in maximum intracellular concentrations ranging from 22.5–90.7% (mgPHA mgVSS−1); at maximum concentration, the mean hydroxyvalerate mol content was 73±0.6%. While a typical feast-famine dissolved oxygen (DO) pattern was observed at maximum aeration, less resolution was observed at decreasing aeration rates, suggesting that DO may not be an optimal process monitoring parameter. At lower aeration states, nitrogen cycling patterns, supported by molecular investigations targeting AOBs and NOBs, indicate that NO2 and NO3 sustained feast-famine PHA synthesis. Next-generation sequencing analysis of the respective MMC revealed numerous and diverse genera exhibiting the potential to achieve PHA synthesis, suggesting functional redundancy embedded in the diverse MMC. Ultimately, results demonstrate that aeration can be controlled in waste-based ADF systems to sustain PHA production potential, while enriching for a diverse MMC that exhibits potential functional redundancy. Reduced aeration could also enhance cost competitiveness of waste-based PHA production, with potential further benefits associated with nitrogen treatment.

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“…In post-denitrification process without carbon source [3], the denitrification can be approximated as endogenous denitrification. Therefore, many researchers [1, 4,5] mainly devote themselves to improving the post-denitrification rate in order to enhance BNR, but pay little attention to surveying the relationship between aeration rates and nutrient removal performance in postdenitrification process.…”

Section: Introduction Rmentioning

confidence: 99%

S107 Characteristics of Nitrogen and Phosphorus Removal in Multiple Post-Denitrification Process with Different Aeration Rates

Deng¹,

Jin-song²,

Qu³

2016

JRST

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ABSTRACT:The nutrient (nitrogen and phosphorus) removal from municipal wastewater was investigated by sequencing batch reactor (SBR) with different aeration rates. The results indicated that the removal performances of nitrogen and phosphorus both increased first and then decreased with increasing aeration rates, and that the aeration rate should be controlled at 25 L.h -1 (dissolved oxygen = 2.7 mg.L -1 ) in order to achieve ideal removal efficiencies of total nitrogen (TN) and total phosphorus (TP). Aeration rate had a prominent effect on the occurrence of nitrification and denitrification (SND), and excessive aeration would weaken nitrogen removal during SND process. In contrast, excessive aeration had no effect on the release rate of the anaerobic phosphorus, and the decrease of these polyhydroxyalkanoates (PHAs) could significantly influence phosphorus uptake, which was very essential for controlling the biological phosphate removal.

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Advancing post-anoxic denitrification for biological nutrient removal

Cited by 100 publications

References 43 publications

Effect of Endogenous Carbon Source on Biological Denitrification Rate

Effect of Endogenous Carbon Source on Biological Denitrification Rate

Polyhydroxyalkanoate synthesis by mixed microbial consortia cultured on fermented dairy manure: Effect of aeration on process rates/yields and the associated microbial ecology

S107 Characteristics of Nitrogen and Phosphorus Removal in Multiple Post-Denitrification Process with Different Aeration Rates

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