Advantages of using microfabricated extracellular electrodes for in vitro neuronal recording

Breckenridge, L. J.; Wilson, Richard J. A.; Connolly, Patricia; Curtis, Adam; Dow, Julian A. T.; Blackshaw, S. E.; Wilkinson, C. D. W.

doi:10.1002/jnr.490420215

Cited by 92 publications

(51 citation statements)

References 31 publications

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“…Robinson, 1968;Israel et al, 1984;Novak and Wheeler, 1986;Breckenridge et al, 1995). In our system, we typically see an increase of a factor of 170 (to Fig.…”

Section: Electrode Platinizationmentioning

confidence: 67%

The neurochip: a new multielectrode device for stimulating and recording from cultured neurons

Maher

Pine

Wright

et al. 1999

Journal of Neuroscience Methods

304

180

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“…Robinson, 1968;Israel et al, 1984;Novak and Wheeler, 1986;Breckenridge et al, 1995). In our system, we typically see an increase of a factor of 170 (to Fig.…”

Section: Electrode Platinizationmentioning

confidence: 67%

The neurochip: a new multielectrode device for stimulating and recording from cultured neurons

Maher

Pine

Wright

et al. 1999

Journal of Neuroscience Methods

304

180

View full text Add to dashboard Cite

“…Wilkinson et al 174,175 used planar MEAs to study single isolated leech neurons in culture. The 100-μm 2 platinized gold microelectrodes in the 64-electrode array detected the external current that flows during an action potential with signal-to-noise ratios of up to 500:1, giving a maximum recorded signal of several millivolts.…”

Section: Extracellular Electrode Arrays For Multisite Recording and Smentioning

confidence: 99%

Biology on a Chip: Microfabrication for Studying the Behavior of Cultured Cells

Tourovskaia

Folch

2003

Crit Rev Biomed Eng

173

140

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The ability to culture cells in vitro has revolutionized hypothesis testing in basic cell and molecular biology research and has become a standard methodology in drug screening and toxicology assays. However, the traditional cell culture methodology-consisting essentially of the immersion of a large population of cells in a homogeneous fluid medium-has become increasingly limiting, both from a fundamental point of view (cells in vivo are surrounded by complex spatiotemporal microenvironments) and from a practical perspective (scaling up the number of fluid handling steps and cell manipulations for high-throughput studies in vitro is prohibitively expensive). Micro fabrication technologies have enabled researchers to design, with micrometer control, the biochemical composition and topology of the substrate, the medium composition, as well as the type of neighboring cells surrounding the microenvironment of the cell. In addition, microtechnology is conceptually well suited for the development of fast, low-cost in vitro systems that allow for high-throughput culturing and analysis of cells under large numbers of conditions. Here we review a variety of applications of microfabrication in cell culture studies, with an emphasis on the biology of various cell types.

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“…A diversity of shapes and a wide range of amplitudes of signals recorded with planar electrodes from different neurons have been reported (Gross, 1979;Regehr et al, 1989;Bove et al, 1995;Breckenridge et al, 1995;Jenkner & Fromherz, 1997;Schatzthauer & Fromherz, 1998;Fromherz, 1999;Ruardij et al, 2009). Signals were generally classified (arranged in types) according to the waveform and amplitude .…”

Section: Fig 1 Schematic View Of An Electrode Covered With Neuronsmentioning

confidence: 99%

Extracellular electrical signals in a neuron-surface junction: model of heterogeneous membrane conductivity

et al. 2012

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Signals recorded from neurons with extracellular planar sensors have a wide range of waveforms and amplitudes. This variety is a result of different physical conditions affecting the ion currents through a cellular membrane. The transmembrane currents are often considered by macroscopic membrane models as essentially a homogeneous process. However, this assumption is doubtful, since ions move through ion channels, which are scattered within the membrane. Accounting for this fact, the present work proposes a theoretical model of heterogeneous membrane conductivity. The model is based on the hypothesis that both potential and charge are distributed inhomogeneously on the membrane surface, concentrated near channel pores, as the direct consequence of the inhomogeneous transmembrane current. A system of continuity equations having non-stationary and quasi-stationary forms expresses this fact mathematically. The present work performs mathematical analysis of the proposed equations, following by the synthesis of the equivalent electric element of a heterogeneous membrane current. This element is further used to construct a model of the cell-surface electric junction in a form of the equivalent electrical circuit. After that a study of how the heterogeneous membrane conductivity affects parameters of the extracellular electrical signal is performed. As the result it was found that variation of the passive characteristics of the cell-surface junction, conductivity of the cleft and the cleft height, could lead to different shapes of the extracellular signals.

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Advantages of using microfabricated extracellular electrodes for in vitro neuronal recording

Cited by 92 publications

References 31 publications

The neurochip: a new multielectrode device for stimulating and recording from cultured neurons

The neurochip: a new multielectrode device for stimulating and recording from cultured neurons

Biology on a Chip: Microfabrication for Studying the Behavior of Cultured Cells

Extracellular electrical signals in a neuron-surface junction: model of heterogeneous membrane conductivity

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