Adventitious Rooting and Browning are Differentially Controlled by Auxin in Rooting-Recalcitrant Elegia capensis (Burm. f.) Schelpe

Verstraeten, Inge; Geelen, Danny

doi:10.1007/s00344-015-9482-0

Cited by 11 publications

(2 citation statements)

References 42 publications

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“…Yu et al [22] and Long et al [23] successfully established the kiwifruit stem culture using MS + 6-BA (1.0 mg/L) + naphthylacetic acid (NAA, 0.1 mg/ L) medium. It was also found that different concentrations of plant growth regulators could affect the growth and browning of kiwifruit, and the auxin IBA could delay the synthesis of polyphenols and reduce browning [24][25][26]. Based on the results of previous literature, this experiment studied the effects of IBA at different concentrations on the induction and proliferation of "Guichang" kiwifruit stem segments.…”

Section: Discussionmentioning

confidence: 98%

Establishment of Tissue Culture System of Actinidia deliciosa Cultivar “Guichang”

Zhong

Zhou

Tang

et al. 2021

Journal of Chemistry

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In order to breed virus-free plantlets of the kiwifruit cultivar “Guichang,” which belongs to Actinidia deliciosa, in this study, stem segments with buds were used as explants, the establishment of a tissue culture rapid propagation system was carried out, and then the virus status of tissue culture plantlets was detected via the real-time reverse transcription-polymerase chain reaction (RT-qPCR) method. The tissue culture rapid propagation system proved that the contamination and browning rates could be controlled below 20% and the survival rate could be exceeded by 70% when the single bud stem segment of “Guichang” kiwifruit was sterilized with 70% alcohol for 30–60 s and 15% NaClO for 15 min, respectively. Meanwhile, we screened the hormone concentration to get better results, and the appropriate medium for adventitious bud induction was MS + 6-BA (1.0 mg/L) + IBA (0.2 mg/L); for proliferation, it was MS + 6-BA (1.0 mg/L) + IBA (0.1 mg/L); and for rooting, it was 1/2 MS + IBA (0.3 mg/L), and the efficiency of induction, proliferation, and rooting could reach 74.07%, 79.63%, and 85.18%, respectively. In addition, the RT-qPCR results demonstrated that the infection rate of 9 viruses: apple stem grooving virus (ASGV), cucumber mosaic virus (CMV), Actinidia virus X (AVX), cucumber necrosis virus (CNV), ribgrass mosaic virus (RMV), citrus leaf blotch virus (CLBV), Actinidia virus B (AcVB), Pelargonium zonate spot virus (PZSV), and cherry leaf roll virus (CLRV) in the “Guichang” kiwifruit tissue culture plantlets was 0. This study could lay a foundation for the production of “Guichang” kiwifruit tissue culture seedlings, and the medium formula provided in this study was useful for the industrial rapid propagation of “Guichang” plantlets.

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Section: Discussionmentioning

confidence: 98%

Establishment of Tissue Culture System of Actinidia deliciosa Cultivar “Guichang”

Zhong

Zhou

Tang

et al. 2021

Journal of Chemistry

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“…(Cardoso and da Silva 2013), Elegia capensis (Burm. f.) Schelpe (Verstraeten and Geelen 2015), and Cariniana legalis (Lerin et al 2021). Rooting-related problems limit the application of in vitro propagation for plant breeding and conservation efforts.…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic analyses provide insight into adventitious root formation of Euryodendron excelsum H. T. Chang during ex vitro rooting

Xiong

Chen

Wei

et al. 2022

Plant Cell Tiss Organ Cult

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Euryodendron excelsum H. T. Chang, a critically endangered species endemic to China, is a source of valuable material for the furniture and construction industries. However, this species has some challenges associated with rooting during in vitro propagation that have yet to be resolved. In this study, we optimized rooting and conducted a transcriptomic analysis to appreciate its molecular mechanism, thereby promoting the practical application of in vitro propagation of E. excelsum, and providing technical support for the ecological protection of this rare and endangered species. Results showed that ex vitro rooting performed the highest rooting percentage with 98.33% at 25 d. During ex vitro rooting, there was a wide uctuation of endogenous levels of indole-3-acetic acid (IAA) and hydrogen peroxide (H 2 O 2 ) at the stage of root primordia formation. Transcriptome analysis revealed multiple differentially expressed genes (DEGs) involved in AR development. DEGs involved in plant hormone signal transduction, such as genes encoding auxin-induced protein, auxin-responsive protein, and IAA-amido synthetase Gretchen Hagen3, and in response to H 2 O 2 , oxidative stress, abiotic and biotic stimuli were signi cantly up-or down-regulated by ex vitro treatment with 1 mM indole-3-butyric acid (IBA). Our results indicate that ex vitro rooting is an effective method to induce AR from E. excelsum plantlets during micropropagation. DEGs involved in the plant hormone signal transduction pathway played a crucial role in AR formation. H 2 O 2 , produced by environmental stimulation, might be related to AR induction as a result of the synergistic action with IBA, ultimately regulating the level of endogenous IAA. Key MessageUnder ex vitro rooting, a synergistic action between H 2 O 2 produced by environmental stimulation and IBA played crucial role in the regulation of AR formation from E. excelsum plantlets during micropropagation.

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Differential responses to isoprenoid, N 6-substituted aromatic cytokinins and indole-3-butyric acid in direct plant regeneration of Eriocephalus africanus

et al. 2017

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Adventitious Rooting and Browning are Differentially Controlled by Auxin in Rooting-Recalcitrant Elegia capensis (Burm. f.) Schelpe

Cited by 11 publications

References 42 publications

Establishment of Tissue Culture System of Actinidia deliciosa Cultivar “Guichang”

Establishment of Tissue Culture System of Actinidia deliciosa Cultivar “Guichang”

Transcriptomic analyses provide insight into adventitious root formation of Euryodendron excelsum H. T. Chang during ex vitro rooting

Differential responses to isoprenoid, N 6-substituted aromatic cytokinins and indole-3-butyric acid in direct plant regeneration of Eriocephalus africanus

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