Adventitious shoot regeneration from dormant buds of persimmon (Diospyros kaki Thunb.) cv. Hachiya

Abstract

<jats:title>Adventitious shoot regeneration from dormant buds of persimmon (<jats:italic>Diospyros kaki</jats:italic>Thunb.) cv. Hachiya</jats:title><jats:p>The effects of plant hormones 6-benzylaminopurine (BAP) and indole-3-butyric acid (IBA) on adventitious shoot regeneration from dormant persimmon buds were studied. The object of the study was the persimmon cultivar Hachiya, one of the most important persimmon cultivars in the Mediterranean area and Asia. Shoot regeneration was evaluated 4, 6 and 8 weeks after initiating the hormone treatment. Average shoot length was measured after 8 weeks and was evaluated by LSD test. Except for the media without hormone supplement, there was a statistically significant difference among average values of shoot length of plants, grown on the tested media. The increase in BAP had an effect on shoot regeneration that was significant and more pronounced with the addition of IBA, especially to the MS (1/2 N) medium. The highest value of shoot regeneration (98%) was obtained on medium MS (1/2 N), supplemented with 5 μmol l<jats:sup>-1</jats:sup>BAP and 1 μmol l<jats:sup>-1</jats:sup>IBA, with the highest average shoot length 23.69 mm, measured 8 weeks after the experiment initiation. The results indicate that adventitious shoots can be successfully produced in persimmon cv. Hachiya, especially with the supplement of hormone BAP, which, according to our results, plays an important role in persimmon<jats:italic>in vitro</jats:italic>regeneration.</jats:p>

Effect of Cytokinins on in Vitro Shoot Proliferation of Olive Cultivars “Earlik” and “Bari Zaitoon-2”

“…Still, it also conjugated with other materials but its amount is relatively smaller as compared to other plant growth regulators (Buah et al, 2010). Although BAP was considered best in inducing shoot as it increased shoot proliferation in various crops like a peach (Arab et al, 2014), kiwi fruit (Akbaş et al, 2007), hazelnut (Thomson and Deering, 2011) and persimmon (Kochanová et al, 2011). But in olive cultivars, Zeatin improves shoot induction in both "Earlik" and "BARI Zaitoon-2" cultivars respectively.…”

Genetic Diversity and Breeding of Persimmon

“…The main in vitro tissue culture techniques developed for persimmon deal with direct regeneration (from dormant buds and root tips) and indirect regeneration through callus from dormant buds, apexes and leaves. Kochanová et al [61] indicated that in the genus Diospyros L., biotechnological researches focused on quality improvement and preservation of the cultivars that has been economically cultivated. The authors also remarked that the genetic variability had been lost as only those limited cultivars that are popular among growers are grown.…”

Efektivitas Pemberian IAA, IBA, NAA, dan Root-up pada Pembibitan Kesemek

“…Penggunaan 0.4% NAA pada bibit kesemek yang dibudidayakan pada media MS dilaporkan sebanyak 25 dari 95 kultivar kesemek gagal membentuk akar, sedangkan penggunaan IBA secara in vitro lebih efektif pada kondisi gelap (Palla et al, 2013). IBA mampu membantu pembentukan akar kesemek secara baik pada kondisi in vitro tetapi tidak pada kondisi in vivo (Kochanová et al, 2011).…”