Adventitious shoot regeneration from in vitro-cultured leaves of Rubus genotypes

Abstract

Adventitious shoot regeneration from in vitro-cultured leaves of Rubus genotypesB a r b a r a A m b r o~i~ T u r k 1, H a r r y J. Swartz 2 & R i c h a r d H. Z i m m e r m a n * AbstractRegeneration of adventitious shoots from leaves of several in vitro-cultured Rubus genotypes was affected by media components and incubation conditions. Leaves cultured at 20°C with a photosynthetic photon flux (PPF) of 40 p.mol m -2 s-1 had a higher regeneration frequency and more shoots per regenerating leaf than ones cultured at 25°C with a PPF of either 40 or 80 lamol m -2 s -l. Thidiazuron (TDZ) was significantly more effective than benzyladenine. Medium containing 1 ~tM TDZ had the highest percentage regeneration for leaves of 'Autumn Bliss', 'Canby', 'Summit' and 'Sentry' red raspberries, whereas leaves of MD-ETCE-1 blackberry hybrid responded more to 10 ~tM TDZ. Higher regeneration frequencies were obtained with 0.5 p_M indolebutyric acid (IBA) than with 1 ~tM, but no significant difference was observed between 0.5 ~tM and no IBA in other experiments. More shoots per regenerating leaf formed on Murashige and Skoog (MS) and N6 media than on half-strength MS, Anderson, or Woody Plant media for all genotypes tested. The youngest two expanding leaves near the shoot apex were the most regenerative and yielded the highest number of shoots per regenerating leaf.

Micropropagation of three berry fruit species using nodal segments from field‐grown plants

“…The deficient sanitary state of stock plants (Table 1) could explain the differences found as a function of collection time, since the necessary growth chamber stimulation of hardwood cuttings facilitated fungal development, preventing the subsequent in vitro culture establishment. The different performance of the two raspberry cultivars indicates an effect of genotype which has already been reported both for Rubus (Turk, Swartz & Zimmerman, 1994;McNicol & Graham, 1990) and Vaccinium species (Reed & Abdelnour-Esquivel, 1991).…”

Adventitious regeneration in blackberry (Rubus fruticosus L.) and assessment of genetic stability in regenerants

“…The regeneration was indirect and adventitious shoots formed mainly from callus that developed around the cut end of petioles and along the first cut across the mid-vein nearest to the petiole. Ambrožič Turk et al (1994) obtained similar results with blackberry hybrid MD-ETCE-1 and three raspberry cultivars.…”

The influence of FeEDDHA in red raspberry cultures during shoot multiplication and adventitious regeneration from leaf explants

“…However, some transgenic plants of raspberry have been obtained (Mathews et al, 1995;McNicol and Graham, 1999;Mezzetti et al, 2004). Significant differences between genotypes have been reported in micropropagation (Reed, 1990) as well as in adventitious regeneration efficiency (Turk et al, 1994;Graham et al, 1997). We wanted to find out how an improvement in the quality of stock cultures, from which leaves were to be obtained, via the elimination of chlorosis with the aid of FeEDDHA, would affect multiplication and if it would increase competence for regeneration.…”