Adverse effect of polystyrene microplastics (PS-MPs) on tube formation and viability of human umbilical vein endothelial cells

Lee, Hee-Seop; Amarakoon, Darshika; Wei, Cheng‐I; Choi, Kyu Yong; Smolensky, Dmitriy; Lee, Seong-Ho

doi:10.1016/j.fct.2021.112356

Cited by 78 publications

(32 citation statements)

References 42 publications

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“…Among these, in vivo studies have revealed disruptions of gut homeostasis (Choi et al 2021b ; Liang et al 2021 ), increased reactive oxygen species generation and inflammation (Ijaz et al 2021 ), genotoxicity and lipotoxicity (Deng et al 2017 ; Gonzalez-Acedo et al 2021 ), and exacerbated bone resorption (Markel et al 2009 ). In vitro experiments (often using human or rodent cell lines) have similarly demonstrated the adverse effects of microplastics, including immune cell activation (Pearl et al 2011 ), inflammatory cytokine release (Schirinzi et al 2017 ), bone resorption activity (Zhang et al 2008 ), decreased survival (Van Tienhoven et al 2006 ), cytostructural perturbations and neurotoxicity (Gonzalez-Acedo et al 2021 ), and loss of phenotypic cellular behaviors (Lee et al 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Polystyrene and Polyethylene Microplastics Decrease Cell Viability and Dysregulate Inflammatory and Oxidative Stress Markers of MDCK and L929 Cells In Vitro

2021

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Microplastics are ubiquitous environmental pollutants that are a growing concern to many ecosystems, as well as human health. Many of the effects of microplastics on mammalian cells and tissues remain unknown. To address this, we treated L929 murine fibroblasts and Madin–Darby canine kidney (MDCK) epithelial cell lines with 1 μg/mL, 10 μg/mL, or 20 μg/mL of polyethylene (PE) or polystyrene (PS) microspheres in vitro for 6 and 24 h and measured the resulting changes in cell viability, metabolism, and transcriptional expression of inflammatory cytokines and antioxidant enzymes. We observed dose-dependent decreases in cell viability corresponding to increases in doses of both PE and PS. We conducted cell metabolism assays and observed dose-dependent increases in metabolism per cell with increasing doses of both PE and PS. Similarly, we also observed increased expression of the superoxide dismutase-3 gene ( SOD3 ), indicating oxidative stress caused by the microplastics treatments. We also observed increased expression of TNFα , but decreased expression of IFNβ , suggesting different mechanisms by which the microplastics regulate inflammatory responses in mammalian cells. Our results contribute new data to the growing understanding of the effects of microplastics on mammalian cells and indicate complex cellular stress responses to microplastics in the environment.

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Section: Introductionmentioning

confidence: 99%

Polystyrene and Polyethylene Microplastics Decrease Cell Viability and Dysregulate Inflammatory and Oxidative Stress Markers of MDCK and L929 Cells In Vitro

2021

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“…Interestingly, in a human vascular endothelial cell line (HUVECs), size-dependent impacts of MPs were also observed. However, in this case, smaller MPs (0.5 µm) were shown to have a greater impact on cell viability in an autophagy-dependent manner, as compared to larger MPs (5.0 µm) [36].…”

Section: Human Effectsmentioning

confidence: 66%

“…For 1 µm sized particles, at 72 h cell viability is impacted in a cell concentration-dependent manner, with 100 µg/mL having the strongest effect. Regardless of concentration, 5 µm particles did not significantly alter cell viability [36]. Although the authors proposed that MPs induce autophagy-dependent cell death and negatively interact with cell-surface receptors, the reasoning for the differences observed between 0.5 µm and 1.0 µm remain unclear.…”

Section: Human Effectsmentioning

confidence: 95%

“…Many experimental studies focusing on MPs in the context of human health either use a single concentration of MPs or use a range that is not environmentally relevant. One recent report studying human cells (HUVECs) used a range of MPs at 0-100 µg/mL [36]. Based on previous findings, these authors argued that this would be a good estimate for the actual concentration of MPs in human blood (~99.4 µg/mL).…”

Section: Human Effectsmentioning

confidence: 99%

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Reality Check: Experimental Studies on Microplastics Lack Realism

Weis

Palmquist

2021

Applied Sciences

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Environmental microplastics are gaining interest due to their ubiquity and the threat they pose to environmental and human health. Critical studies have revealed the abundance of microplastics in nature, while others have tested the impacts of these small plastics on organismal health in the laboratory. Yet, there is often a mismatch between these two areas of research, resulting in major discrepancies and an inability to interpret certain findings. Here, we focus on several main lines of inquiry. First, even though the majority of environmental microplastics are plastic microfibers from textiles, laboratory studies still largely use spherical microbeads. There are also inconsistencies between the measurements of microplastics in the environment as compared to the concentrations that tend to be used in experimental studies. Likewise, the period of exposure occurring in experimental studies and in the environment are vastly different. Lastly, although experimental studies often focus on a particular subset of toxic chemicals present on microplastics, textile microfibers carry other dyes and chemicals that are understudied. They also cause types of physical damage not associated with microspheres. This review will analyze the literature pertaining to these mismatches, focusing on aquatic organisms and model systems, and seek to inform a path forward for this burgeoning area of research.

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“…Tube formation was measured as described previously 25 . Briefly, cells (10 000 cells per well) were plated in a 96‐well cell culture dish pre‐coated with ECMatrix gel (50 μL per well) for 1 h, then incubated for 6 h with the concentrations of permethrin indicated.…”

Section: Methodsmentioning

confidence: 99%

Permethrin inhibits tube formation and viability of endothelial cells

et al. 2022

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BACKGROUND Exposure to environmental chemicals has been linked with endothelial dysfunction, which is a leading cause of human diseases, including atherosclerosis. Permethrin is a frequently used synthetic pyrethroid insecticide for which longer exposure may cause toxicity in several types of tissues and the development of metabolic diseases, including atherosclerosis, obesity and diabetes. The present study was designed to evaluate the potential adverse effect of permethrin on the function and activity of human endothelial cells. RESULTS Permethrin was found to repress migration and tube formation by human umbilical vein endothelial cells (HUVECs) in a dose‐dependent manner, as well as to significantly repress their viability after 24 and 48 h of treatment. Furthermore, increased reactive oxygen species (ROS) production was observed in cells treated with permethrin, and the permethrin‐induced repression of cell viability was ROS‐dependent. Permethrin did not influence apoptosis, necrosis or mitochondrial membrane potential in HUVECs. CONCLUSION The results of the present study suggest that permethrin represses angiogenesis and viability through ROS‐dependent and cell growth‐, apoptosis‐ and necrosis‐independent means. © 2022 Society of Chemical Industry.

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Adverse effect of polystyrene microplastics (PS-MPs) on tube formation and viability of human umbilical vein endothelial cells

Cited by 78 publications

References 42 publications

Polystyrene and Polyethylene Microplastics Decrease Cell Viability and Dysregulate Inflammatory and Oxidative Stress Markers of MDCK and L929 Cells In Vitro

Polystyrene and Polyethylene Microplastics Decrease Cell Viability and Dysregulate Inflammatory and Oxidative Stress Markers of MDCK and L929 Cells In Vitro

Reality Check: Experimental Studies on Microplastics Lack Realism

Permethrin inhibits tube formation and viability of endothelial cells

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