Aerobic, anaerobic and fungal burn wound infections

Mousa, Haider Abdul-Lateef

doi:10.1016/s0195-6701(97)90148-1

Cited by 94 publications

(58 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The predominant bacteria and fungi were P. aeruginosa and A. niger, respectively, and observed in 11 (64.7%) patients, while other mixed bacteria and fungi were recorded in the remaining 6 patients (33.3%). Mousa 64 reported that polymicrobial bacterial and fungal burn wound infections were observed in 57.5%. P. aeruginosa was the most common isolate (19.1%) and the predominant fungi were Aspergillus sp.…”

Section: Incidence Of Monomicrobial and Polymicrobial Infectionsmentioning

confidence: 99%

Synthesized zinc peroxide nanoparticles (ZnO<sub>2</sub>-NPs): a novel antimicrobial, anti-elastase, anti-keratinase, and anti-inflammatory approach toward polymicrobial burn wounds

Ali¹,

Morsy²,

El-Zawawy³

et al. 2017

IJN

121

View full text Add to dashboard Cite

Increasing of multidrug resistance (MDR) remains an intractable challenge for burn patients. Innovative nanomaterials are also in high demand for the development of new antimicrobial biomaterials that inevitably have opened new therapeutic horizons in medical approaches and lead to many efforts for synthesizing new metal oxide nanoparticles (NPs) for better control of the MDR associated with the polymicrobial burn wounds. Recently, it seems that metal oxides can truly be considered as highly efficient inorganic agents with antimicrobial properties. In this study, zinc peroxide NPs (ZnO 2 -NPs) were synthesized using the co-precipitation method. Synthesized ZnO 2 -NPs were characterized by X-ray diffraction, Fourier transformed infrared, transmission electron microscopy, thermogravimetric analysis, differential scanning calorimetry, and ultraviolet-visible spectroscopy. The characterization techniques revealed synthesis of the pure phase of non-agglomerated ZnO 2 -NPs having sizes in the range of 15–25 nm with a transition temperature of 211°C. Antimicrobial activity of ZnO 2 -NPs was determined against MDR Pseudomonas aeruginosa (PA) and Aspergillus niger (AN) strains isolated from burn wound infections. Both strains, PA6 and AN4, were found to be more susceptible strains to ZnO 2 -NPs. In addition, a significant decrease in elastase and keratinase activities was recorded with increased concentrations of ZnO 2 -NPs until 200 µg/mL. ZnO 2 -NPs revealed a significant anti-inflammatory activity against PA6 and AN4 strains as demonstrated by membrane stabilization, albumin denaturation, and proteinase inhibition. Moreover, the results of in vivo histopathology assessment confirmed the potential role of ZnO 2 -NPs in the improvement of skin wound healing in the experimental animal models. Clearly, the synthesized ZnO 2 -NPs have demonstrated a competitive capability as antimicrobial, anti-elastase, anti-keratinase, and anti-inflammatory candidates, suggesting that the ZnO 2 -NPs are promising metal oxides that are potentially valued for biomedical applications.

show abstract

Section: Incidence Of Monomicrobial and Polymicrobial Infectionsmentioning

confidence: 99%

Synthesized zinc peroxide nanoparticles (ZnO<sub>2</sub>-NPs): a novel antimicrobial, anti-elastase, anti-keratinase, and anti-inflammatory approach toward polymicrobial burn wounds

Ali¹,

Morsy²,

El-Zawawy³

et al. 2017

IJN

121

View full text Add to dashboard Cite

show abstract

“…In fact, the co-isolation of these diverse organisms from an array of acute and chronic diseases such as burn wounds, ventilator-associated pneumonia and bloodstream infections is well documented (Combes et al, 2002;Klotz et al, 2007a;Mousa, 1997). The majority of these clinical conditions are considered to be biofilm-associated, whereby adherence to and colonization of a surface by the microorganism is a prerequisite for the development of the infectious process (Archer et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Staphylococcus aureus adherence to Candida albicans hyphae is mediated by the hyphal adhesin Als3p

et al. 2012

View full text Add to dashboard Cite

The bacterium Staphylococcus (St.) aureus and the opportunistic fungus Candida albicans are currently among the leading nosocomial pathogens, often co-infecting critically ill patients, with high morbidity and mortality. Previous investigations have demonstrated preferential adherence of St. aureus to C. albicans hyphae during mixed biofilm growth. In this study, we aimed to characterize the mechanism behind this observed interaction. C. albicans adhesin-deficient mutant strains were screened by microscopy to identify the specific receptor on C. albicans hyphae recognized by St. aureus. Furthermore, an immunoassay was developed to validate and quantify staphylococcal binding to fungal biofilms. The findings from these experiments implicated the C. albicans adhesin agglutinin-like sequence 3 (Als3p) in playing a major role in the adherence process. This association was quantitatively established using atomic force microscopy, in which the adhesion force between single cells of the two species was significantly reduced for a C. albicans mutant strain lacking als3. Confocal microscopy further confirmed these observations, as St. aureus overlaid with a purified recombinant Als3 N-terminal domain fragment (rAls3p) exhibited robust binding. Importantly, a strain of Saccharomyces cerevisiae heterologously expressing Als3p was utilized to further confirm this adhesin as a receptor for St. aureus. Although the parental strain does not bind bacteria, expression of Als3p on the cell surface conferred upon the yeast the ability to strongly bind St. aureus. To elucidate the implications of these in vitro findings in a clinically relevant setting, an ex vivo murine model of co-infection was designed using murine tongue explants. Fluorescent microscopic images revealed extensive hyphal penetration of the epithelium typical of C. albicans mucosal infection. Interestingly, St. aureus bacterial cells were only seen within the Abbreviations: AFM, atomic force microscopy; CLSM, confocal laser scanning microscopy; ConA-Texas red, concanavalin A conjugated to Texas red; H&E, haematoxylin and eosin; IFA, indirect immunofluorescence; PNA-FISH, peptide nucleic acid probes for fluorescent in situ hybridization; SEM, scanning electron microscopy. A supplementary figure is available with the online version of this paper. Microbiology

show abstract

“…aeruginosa is among the most common causes of severe infection in burnt patients [1,2]. Despite the variable degrees of burns, infection remains the most common cause of mortality amongst burnt patients [3].…”

Section: Introductionmentioning

confidence: 99%

Isolation and Identification of Multi-drug resistant Pseudomonas aeruginosa from Burn Wound Infection in Chittagong City, Bangladesh

Nath¹,

Chowdhury²,

Dhar³

et al. 2017

IOSRJPBS

View full text Add to dashboard Cite

show abstract

Aerobic, anaerobic and fungal burn wound infections

Cited by 94 publications

References 16 publications

Synthesized zinc peroxide nanoparticles (ZnO<sub>2</sub>-NPs): a novel antimicrobial, anti-elastase, anti-keratinase, and anti-inflammatory approach toward polymicrobial burn wounds

Synthesized zinc peroxide nanoparticles (ZnO<sub>2</sub>-NPs): a novel antimicrobial, anti-elastase, anti-keratinase, and anti-inflammatory approach toward polymicrobial burn wounds

Staphylococcus aureus adherence to Candida albicans hyphae is mediated by the hyphal adhesin Als3p

Isolation and Identification of Multi-drug resistant Pseudomonas aeruginosa from Burn Wound Infection in Chittagong City, Bangladesh

Contact Info

Product

Resources

About