Aeromicrobium endophyticum sp. nov., an endophytic actinobacterium isolated from reed (Phragmites australis)

Li, Fei-Na; Liao, Shuilin; Liu, Shaowei; Jin, Tao; Sun, Cheng‐Hang

doi:10.1007/s12275-019-8705-7

Cited by 24 publications

(14 citation statements)

References 40 publications

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“…The cells of Aeromicrobium are characterized as aerobic, Gram-stain-positive, non-spore-forming, coccoid or rodshaped with optimal growth at 25-35 °C [2]. Moreover, the cells contain ll-diaminopimelic acid (ll-DAP) as the diagnostic diamino acid, MK-9(H 4 ) as the predominant menaquinone, C 18 : 0 10-methyl and C 16 : 0 together with either C 18 : 1 ω9c or C 16 : 0 2-OH as the major fatty acids [2][3][4][5]. At the time of writing, 16 species of Aeromicrobium had been published with valid names in an authoritative database ( www.…”

Section: Introductionmentioning

confidence: 99%

“…An additional species named 'Aeromicrobium halotolerans' , without valid names, was also published in the literature [6]. Members of the genus were initially isolated from various habitats [1][2][3][4][5][6][7][8][9][10], including different environments (soils, marine water and air) and animals (human and warbler). We recently isolated two novel species of the family Nocardioidaceae named Nocardioides houyundeii [11] and Nocardioides yefusunii [12] from wild animals living on the Tibet-Qinghai Plateau.…”

Section: Introductionmentioning

confidence: 99%

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Aeromicrobium chenweiae sp. nov. and Aeromicrobium yanjiei sp. nov., isolated from Tibetan antelope (Pantholops hodgsonii) and plateau pika (Ochotona curzoniae), respectively

Lei

Yang

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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Four novel strains (592T, S592, MF47T and SMF47) were isolated from Tibetan antelopes (Pantholops hodgsonii) and plateau pikas (Ochotona curzoniae), respectively. The cells were aerobic, non-motile, Gram-stain- and catalase-positive, rod-shaped bacteria. The 16S rRNA gene sequences of the four strains showed highest similarities to Aeromicrobium fastidiosum DSM 10552T (98.1, 98.6, 98.7 and 98.7 %, respectively), and the phylogenetic analyses based on 16S rRNA gene and genomic sequences indicated that strains 592T and MF47T represent two novel species. The four isolates produced acid from l-rhamnose, d-xylose and cellobiose, but were unable to reduce nitrate. The DNA G+C contents of strains 592T and MF47T were 70.3 and 69.8 mol%, respectively. The digital DNA–DNA hybridization value between strains 592T and MF47T was 32.6 %, lower than the threshold of 70 %, indicating they belong to different species. The four strains’ genomes displayed less than 24.6 % DNA–DNA relatedness with all available genomes of the genus Aeromicrobium in the NCBI database, including Aeromicrobium fastidiosum NBRC 14897T and Aeromicrobium ginsengisoli JCM 14732T. The major fatty acids of the four strains were C18 : 1 ω9c and C18 : 0 10-methyl, and the main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. The predominant respiratory quinones were MK-9(H4) and MK-8(H4). The cell-wall peptidoglycan contained ll-diaminopimelic acid. Based on these genotypic, phenotypic and biochemical analyses, it is proposed that the four unidentified bacteria be classified as two novel species, Aeromicrobium chenweiae sp. nov. and Aeromicrobium yanjiei sp. nov. The type strains are 592T (=CGMCC1.16526T=DSM 106289T) and MF47T (=CGMCC 1.17444T=JCM 33790T), respectively.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Aeromicrobium chenweiae sp. nov. and Aeromicrobium yanjiei sp. nov., isolated from Tibetan antelope (Pantholops hodgsonii) and plateau pika (Ochotona curzoniae), respectively

Lei

Yang

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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“…These strains will be further identified with a polyphasic approach to determine their taxonomic positions. In addition, three strains with relatively high 16S rRNA sequence similarities, i.e., 11W25H-1 T , 8H24J-4-2 T , and 9W16Y-2 T , had been identified as type species and named as Labedella phragmitis , Labedella populi , and Aeromicrobium endophyticum , respectively [ 28 , 29 ]. In this study, novelty analysis suggested that desert plants have the potential as excellent sources of novel actinobacterial species.…”

Section: Resultsmentioning

confidence: 99%

Diversity, novelty, antimicrobial activity, and new antibiotics of cultivable endophytic actinobacteria isolated from psammophytes collected from Taklamakan Desert

Wang

et al. 2021

Journal of Pharmaceutical Analysis

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Three hundred and twenty endophytic actinobacterial strains were isolated from psammophytes collected from Taklamakan Desert and identified. Among them, three strains already had been identified as new species of two genera and sixteen isolates showed relatively low 16S rRNA similarities < 98.6% to validly described species. Seventy-five of the isolates were selected as representative strains to screen antibacterial activity and mechanism. Forty-seven strains showed antagonistic activity against at least one of the indicator bacteria. Two Streptomyces strains produced bioactive compounds inducing DNA damage, and two Streptomyces strains produced bioactive compounds with inhibitory activity on protein biosynthesis. Notably, the strain Streptomyces sp. 8P21H-1 that demonstrated both strong antibacterial activity and inhibitory activity on protein biosynthesis was prioritized for exploring new antibiotics. Under the strategy of integrating genetics-based discovery program and MS/MS-based molecular networking, two new streptogramin-type antibiotics, i.e., acetyl-griseoviridin and desulphurizing griseoviridin, along with known griseoviridin, were isolated from the culture broth of strain 8P21H-1. Their chemical structures were determined by HR-MS, and 1D and 2D NMR. Desulphurizing griseoviridin and griseoviridin exhibited antibacterial activities by inhibiting translation.

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“…More recently, PC has been found in significant amounts in membranes of diverse Gram-negative bacteria living in association with eukaryotes for nitrogen fixation (mainly Rhizobiales and Rhodobacterales) and in those of some Gram-positive bacteria [42][43][44]. Of Gram-positive bacteria, Actinobacteria isolated from reed or soil contain PC as one of the major polar lipids [45,46]. In Gram-positive Lactobacillus species, based on lipidomic profiling on L. acidophilus and L. gasseri, some lipid species of PC and LPC were significantly elevated in L. gasseri compared to L. acidophilus (Supplementary data) [47].…”

Section: Discussionmentioning

confidence: 99%

Comparative Lipidomic Analysis of Extracellular Vesicles Derived from Lactobacillus plantarum APsulloc 331261 Living in Green Tea Leaves Using Liquid Chromatography-Mass Spectrometry

Kim

Myoung

et al. 2020

IJMS

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Lactobacillus plantarum is a popular probiotic species due to its safe and beneficial effects on humans; therefore, novel L. plantarum strains have been isolated and identified from various dietary products. Given that bacteria-derived extracellular vesicles (EVs) have been considered as efficient carriers of bioactive materials and shown to evoke cellular responses effectively, L. plantarum-derived EVs are expected to efficiently elicit health benefits. Herein, we identified L. plantarum APsulloc 331261 living in green tea leaves and isolated EVs from the culture medium. We performed quantitative lipidomic analysis of L. plantarum APsulloc 331261 derived EVs (LEVs) using liquid chromatography-mass spectrometry. In comparison to L. plantarum APsulloc 331261, in LEVs, 67 of 320 identified lipid species were significantly increased and 19 species were decreased. In particular, lysophosphatidylserine(18:4) and phosphatidylcholine(32:2) were critically increased, showing over 21-fold enrichment in LEVs. In addition, there was a notable difference between LEVs and the parent cells in the composition of phospholipids. Our results suggest that the lipidomic profile of bacteria-derived EVs is different from that of the parent cells in phospholipid content and composition. Given that lipids are important components of EVs, quantitative and comparative analyses of EV lipids may improve our understanding of vesicle biogenesis and lipid-mediated intercellular communication within or between living organisms.

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Aeromicrobium endophyticum sp. nov., an endophytic actinobacterium isolated from reed (Phragmites australis)

Cited by 24 publications

References 40 publications

Aeromicrobium chenweiae sp. nov. and Aeromicrobium yanjiei sp. nov., isolated from Tibetan antelope (Pantholops hodgsonii) and plateau pika (Ochotona curzoniae), respectively

Aeromicrobium chenweiae sp. nov. and Aeromicrobium yanjiei sp. nov., isolated from Tibetan antelope (Pantholops hodgsonii) and plateau pika (Ochotona curzoniae), respectively

Diversity, novelty, antimicrobial activity, and new antibiotics of cultivable endophytic actinobacteria isolated from psammophytes collected from Taklamakan Desert

Comparative Lipidomic Analysis of Extracellular Vesicles Derived from Lactobacillus plantarum APsulloc 331261 Living in Green Tea Leaves Using Liquid Chromatography-Mass Spectrometry

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