Affinity-Mass Spectrometry Approaches for Elucidating Structures and Interactions of Protein–Ligand Complexes

Petre, Brînduşa Alina

doi:10.1007/978-3-319-06068-2_7

Cited by 4 publications

(1 citation statement)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Affinity mass spectrometry, for example, is an effective method to efficiently find small molecular ligands with strong affinity to target proteins. It can efficiently find ligands with strong binding to target proteins from complex mixtures [ 153 ]. Fluorescence polarization assay is mainly based on the principle of positive correlation between the intensity of polarized fluorescence and the size of fluorescent molecules, which is widely used in high-throughput drug screening and drug analysis [ 154 ].…”

Section: Discussionmentioning

confidence: 99%

Screening S protein – ACE2 blockers from natural products: Strategies and advances in the discovery of potential inhibitors of COVID-19

Liu

et al. 2021

European Journal of Medicinal Chemistry

View full text Add to dashboard Cite

The Coronavirus disease, 2019 (COVID-19) is caused by severe acute respiratory syndrome Coronavirus 2 (SARS‐CoV‐2), which poses a major threat to human life and health. Given its continued development, limiting the spread of COVID-19 in the population remains a challenging task. Currently, multiple therapies are being tried around the world to deal with SARS-CoV-2 infection, and a variety of studies have shown that natural products have a significant effect on COVID-19 patients. The combination of SARS-CoV-2 S protein with Angiotensin converting enzyme II(ACE2) of host cell to promote membrane fusion is an initial critical step for SARS-CoV-2 infection. Therefore, screening natural products that inhibit the binding of SARS-CoV-2 S protein and ACE2 also provides a feasible strategy for the treatment of COVID-19. Establishment of high throughput screening model is an important basis and key technology for screening S protein-ACE2 blockers. Based on this, the molecular structures of SARS-CoV-2 and ACE2 and their processes in the life cycle of SARS-CoV-2 and host cell infection were firstly reviewed in this paper, with emphasis on the methods and techniques of screening S protein-ACE2 blockers, including Virtual Screening (VS), Surface Plasmon Resonance (SPR), Biochromatography, Biotin-avidin with Enzyme-linked Immunosorbent assay and Gene Chip Technology. Furthermore, the technical principle, advantages and disadvantages and application scope were further elaborated. Combined with the application of the above screening technologies in S protein-ACE2 blockers, a variety of natural products, such as flavonoids, terpenoids, phenols, alkaloids, were summarized, which could be used as S protein-ACE2 blockers, in order to provide ideas for the efficient discovery of S protein-ACE2 blockers from natural sources and contribute to the development of broad-spectrum anti coronavirus drugs.

show abstract

Section: Discussionmentioning

confidence: 99%

Screening S protein – ACE2 blockers from natural products: Strategies and advances in the discovery of potential inhibitors of COVID-19

Liu

et al. 2021

European Journal of Medicinal Chemistry

View full text Add to dashboard Cite

show abstract

Mass spectrometric studies of the variety of beta‐amyloid proteoforms in Alzheimer's disease

Zakharova

Kononikhin

Indeykina

et al. 2022

Mass Spectrometry Reviews

View full text Add to dashboard Cite

This review covers the results of the application of mass spectrometric (MS) techniques to study the diversity of beta‐amyloid (Aβ) peptides in human samples. Since Aβ is an important hallmark of Alzheimer's disease (AD), which is a socially significant neurodegenerative disorder of the elderly worldwide, analysis of its endogenous variations is of particular importance for elucidating the pathogenesis of AD, predicting increased risks of the disease onset, and developing effective therapy. MS approaches have no alternative for the study of complex samples, including a wide variety of Aβ proteoforms, differing in length and modifications. Approaches based on matrix‐assisted laser desorption/ionization time‐of‐flight and liquid chromatography with electrospray ionization tandem MS are most common in Aβ studies. However, Aβ forms with isomerized and/or racemized Asp and Ser residues require the use of special methods for separation and extra sensitive and selective methods for detection. Overall, this review summarizes current knowledge of Aβ species found in human brain, cerebrospinal fluid, and blood plasma; focuses on application of different MS approaches for Aβ studies; and considers the potential of MS techniques for further studies of Aβ‐peptides.

show abstract

Immuno-Affinity Mass Spectrometry: A Novel Approaches with Biomedical Relevance

Ion

Petre

2019

Advances in Experimental Medicine and Biology

View full text Add to dashboard Cite

Affinity-Mass Spectrometry Approaches for Elucidating Structures and Interactions of Protein–Ligand Complexes

Cited by 4 publications

References 52 publications

Screening S protein – ACE2 blockers from natural products: Strategies and advances in the discovery of potential inhibitors of COVID-19

Screening S protein – ACE2 blockers from natural products: Strategies and advances in the discovery of potential inhibitors of COVID-19

Mass spectrometric studies of the variety of beta‐amyloid proteoforms in Alzheimer's disease

Immuno-Affinity Mass Spectrometry: A Novel Approaches with Biomedical Relevance

Contact Info

Product

Resources

About