Aflatoxin M1 in milk: Reliability of the immunoenzymatic assay

Rosi, P.; Borsari, Andrea; Lasi, Graziella; Lodi, Sandro; Galanti, A.; Fava, Aldo Felipe; Girotti, Stefano; Ferri, Elida Nora

doi:10.1016/j.idairyj.2006.05.003

Cited by 40 publications

(36 citation statements)

References 27 publications

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“…The interday and inter-level mean recovery value, which was used to correct routine analysis results, was 71.4%. The recovery values achieved in this study were lower than those reported by other authors [11,12,19]. However, they are still in agreement with the performance criteria defined in 401/2006/EC [24], which recommends values in the range of 60-120% for concentrations in the range 0.010-0.050 µg kg -1 , and 70-110% for concentrations above 0.050 µg kg -1 .…”

Section: T a B L Esupporting

confidence: 72%

“…The calculated LOD of 0.0066µg kg -1 (Table 1) was significantly lower than the MRL value for AFM 1 (0.050 µg kg -1 ), thus avoiding the false negative results, and obtaining a reasonable number of false positives. The obtained values were higher than those published by other authors [19,30,31]. This was expected, bearing in mind the fact that other authors have optimized the ELISA method by performing the experiments only on bovine milk.…”

Section: Limit Of Detection Limit Of Quantification and Detection Camentioning

confidence: 42%

“…In the past, several procedures for the determination of AFM 1 have been developed, comprising immunochemical methods [17][18][19][20][21][22], such as enzyme-linked immunosorbent assays (ELISA), which are commonly used for screening purposes within intensive surveillance programs. The method should be rapid, simple, with less sample cleanup procedures, high throughput and performances that comply with the requirements laid down in the ISO Standard [9].…”

Section: Introductionmentioning

confidence: 99%

“…In spite of the many published articles for ELISA screening for the determination of AFM 1 in milk samples, only a few comprehensive validation studies have been reported [18,19,21,22], which still do not strictly follow the Decision 2002/657/EC [25] and ISO Standard requirements [9]. In this paper, an in-house evaluation of the performance characteristics of a commercial ELISA kit for the rapid screening of AFM 1 in raw milk samples following the related regulative [9,24,25] was performed.…”

Section: Introductionmentioning

confidence: 99%

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Study on the method performances for screening and confirmatory analysis of aflatoxin M1 in raw milk according to the mycotoxin regulative requirements

Dimitrieska-Stojkovik

Ilievska

Stojanovska-Dimzoska

et al. 2014

Maced. J. Chem. Chem. Eng.

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Screening and confirmatory methods for aflatoxin M 1 (AFM 1 ) analysis were optimized and studied regarding the European Decision 2002/657/EC requirements. The recoveries of AFM 1 from the spiked samples at levels between 0.025 and 0.075 µg kg -1 were 99.6-110.3% with precision of 10.5-13.4%, confirming the immunochemical method precision and reliability for intensive surveillance studies. According to the regulatory requirements, for confirmation of the obtained positives from the screening, the reference high-performance liquid chromatographic procedure was evaluated in reference to the performance criteria. The between-day recovery obtained was in the range 67.2-72.8%, and the precision range was 5.4-9.1%. Two hundred and twenty five positives from the screening were subjected to confirmatory analysis. Comparison of the data obtained from the immunoassay and chromatographic method (R 2 = 0.764) revealed a slight overestimation of the screening method, but no cases of false-negative determinations occurred. Во овој труд се оптимизирани и проучeни скринингот и методот за потврдување анализа на афлатоксин М 1 (AFM 1 ) со оглед на барањата на Одлуката 2002/657/EC на Европската комисија. Oд спикуваните примероци на ниво на концентрации помеѓу 0,025 и 0,075 µg kg -1 се добиени аналитички приноси за AFM 1 во опсег од 99,6 до 110,3 %, при што утврдената прецизност се движи во опсегот од 10,5 % до 13,4 %. На тој начин е потврдена прецизноста и сигурноста на имунохемискиот метод за негова примена при интензивни надзорни студии на голем број примероци. За релевантно потврдување на позитивните резултати од скринингот е неопходно да се изврши процена на изведбата на референтниот високо ефикасен течно-хроматографски метод, земајќи ги предвид критериумите утврдени во регулативата. Аналитичкиот принос кој е определен во текот на два дена се движи во опсегот од 67,2 до 72,8 % и прецизноста од 5,4 % до 9,1 %. Со примена на потврдниот хроматографски метод повторно беа анализирани 205 позитивни примероци од скринингот. Споредбата на податоците од резултатите добиени со скринингот и потврдниот метод (R 2 = 0,764) покажа дека концентрациите на АFM 1 кои се определени со скринингот во одредена мера се повисоки, но не се утврдени лажно негативни резултати.

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Section: T a B L Esupporting

confidence: 72%

Section: Limit Of Detection Limit Of Quantification and Detection Camentioning

confidence: 42%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Study on the method performances for screening and confirmatory analysis of aflatoxin M1 in raw milk according to the mycotoxin regulative requirements

Dimitrieska-Stojkovik

Ilievska

Stojanovska-Dimzoska

et al. 2014

Maced. J. Chem. Chem. Eng.

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“…Aflatoxins represent a serious risk for animal and human health, especially for children, who are the major milk consumers [5].The hepatotoxic, genotoxic, carcinogenic, teratogenic, immunosuppressive and antinutritional effects of aflatoxins are well documented [6]. Aflatoxins are considered to be human liver carcinogens, Aflatoxin B1 being the most potent.…”

Section: Introductionmentioning

confidence: 99%

Detection of Aflatoxin M1 in Pasteurized Canned Milk and Using of UV Radiation for Detoxification

2017

IJACEBS

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Abstract--The current study was designed to investigate the presence of aflatoxin M1 in 25 samples of pasteurized canned milk which collected randomly from some Iraqi local markets using ELISA technique.Aflatoxin M1 was present in 21 samples, the concentration of aflatoxin M1 ranged from (0.25-50 ppb). UV radiation (365nm wave length) was used for detoxification of aflatoxin M1 (sample with highest concentration /50 ppb of aflatoxin M1 in two different volumes ((25 & 50 ml)) for two different time (15 & 30 min) and 30, 60, 90 cm distance between lamp and milk layer were used for this purpose). Results showed that distance between lamp and milk layer was the most effective parameter in reduction of aflatoxin M1, and whenever the distance increase the effect also increase.

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