Aflatoxin Measurement and Analysis

Li, Peiwu; Zhang, Qi; Zhang, Daohong; Guan, Di; Xiaoxia,; Liu, Ding Xuefen; Fang, Sufang; Wang, Xiupin; Zhang, Wen

doi:10.5772/23954

Cited by 20 publications

(16 citation statements)

References 81 publications

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“…High-performance liquid chromatography (HPLC) is the most popular chromatographic technique for separation and determination of organic compounds. About 80% of organic compounds in the world are determined using HPLC [66]. The HPLC technique makes use of a stationary phase confined to either a glass or a plastic tube and a mobile phase comprising aqueous/organic solvents, which flow through the solid adsorbent.…”

Section: High-performance Liquid Chromatography (Hplc)mentioning

confidence: 99%

“…However, the disadvantage of using HPLC for aflatoxins analysis is the requirement of rigorous sample purification using immunoaffinity columns. In addition, HPLC requires tedious pre-and postcolumn derivatization processes to improve the detection limits of aflatoxins B 1 and G 1 [66]. Therefore, to overcome the challenges associated with derivatization processes in aflatoxins analysis, a modification of the HPLC method, whereby the HPLC is coupled to mass spectroscopy, has been made and is currently employed in the determination of aflatoxin [71].…”

Section: High-performance Liquid Chromatography (Hplc)mentioning

confidence: 99%

See 1 more Smart Citation

Methods for Detection of Aflatoxins in Agricultural Food Crops

Wacoo

Wendiro²,

Vuzi

et al. 2014

Journal of Applied Chemistry

202

149

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Aflatoxins are toxic carcinogenic secondary metabolites produced predominantly by two fungal species: Aspergillus flavus and Aspergillus parasiticus. These fungal species are contaminants of foodstuff as well as feeds and are responsible for aflatoxin contamination of these agro products. The toxicity and potency of aflatoxins make them the primary health hazard as well as responsible for losses associated with contaminations of processed foods and feeds. Determination of aflatoxins concentration in food stuff and feeds is thus very important. However, due to their low concentration in foods and feedstuff, analytical methods for detection and quantification of aflatoxins have to be specific, sensitive, and simple to carry out. Several methods including thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), mass spectroscopy, enzyme-linked immune-sorbent assay (ELISA), and electrochemical immunosensor, among others, have been described for detecting and quantifying aflatoxins in foods. Each of these methods has advantages and limitations in aflatoxins analysis. This review critically examines each of the methods used for detection of aflatoxins in foodstuff, highlighting the advantages and limitations of each method. Finally, a way forward for overcoming such obstacles is suggested.

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Section: High-performance Liquid Chromatography (Hplc)mentioning

confidence: 99%

Section: High-performance Liquid Chromatography (Hplc)mentioning

confidence: 99%

Methods for Detection of Aflatoxins in Agricultural Food Crops

Wacoo

Wendiro²,

Vuzi

et al. 2014

Journal of Applied Chemistry

202

149

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“…In conclusion, the mass fraction of aflatoxin B1 in the Tsabana sample from Food Botswana Serowe was calculated to be 0.05147 ± 0.00135 ng/g (see equations [7][8][9][10]. The quantity of aflatoxin B1 present in the sample was found to be below the European Union regulated level (0.1 ng/g).…”

Section: Application To Real Samplesmentioning

confidence: 80%

“…For an initial volume of the unknown (V 0 ) and an added volume of the standard (V S ) with concentration [S] i , the total volume is given by V = V 0 + V S and the concentrations are given by: [8] And recovery is given by:…”

Section: Pre-concentration Of Afb1 From the Tsabana Extracts Using Thmentioning

confidence: 99%

Development of an aflatoxin B1 specific molecularly imprinted solid phase extraction sorbent for the selective pre-concentration of toxic aflatoxin B1 from child weaning food, Tsabana

Semong¹,

Batlokwa²

2017

Molecular Imprinting

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This paper presents the synthesis, optimization and application of a molecularly imprinted polymer (MIP) sorbent for the selective extraction and pre-concentration of the potent toxin, aflatoxin B1 (AFB1), from the child weaning food, Tsabana (manufactured in Serowe, Botswana). As a food safety regulatory measure, Tsabana must be cleared of hazardous aflatoxins, especially AFB1, before consumption. This is because AFB1 is the most common and potent of the aflatoxins commonly found in cereals. Accurate analysis of AFB1 is challenging because it exists in very low concentrations in complex, ‘dirty’ matrices such as food, making it difficult to detect using analytical instruments, even if these analytical techniques have sensitivities at the femto level. The MIP extraction sorbent synthesized in this paper deals with these challenges by selectively pre-concentrating AFB1 from real Tsabana samples, successfully achieving a pre-concentration factor of 5 and therefore significantly increasing ABF1 signal intensity for easier detection. Further advantages of this system include the short time (25.0 minutes) and reasonable optimal MIP dose (20.0 mg) needed for maximum AFB1 extraction by the sorbent. Scanning electron microscopy revealed that the prepared AFB1 powder particles have spherical geometries and reasonably small sizes (800 nm), two advantageous physical characteristics that are associated with excellent sorbent materials.

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“…The mycotoxin aflatoxin B1 (AFB1) was chosen as the model analyte. 27 Although various methods have been developed for AFB1 detection, such as instrumental analysis using high-performance liquid chromatography (HPLC) 28 or liquid chromatography coupled to mass spectrometry (LC-MS) 29 and different immunological assays, [30][31][32] even more simple and rapid methods are still desirable. AFB1-BSA conjugate modified magnetic beads (AFB1-BSA-Fe 3 O 4 , MBs) were employed as capture probe, while anti-AFB1 antibody-coated AuNPs (Ab-AuNPs) were used as detection probe for immunological recognition of AFB1, as well as for signal transduction.…”

Section: Introductionmentioning

confidence: 99%

Controlled growth of immunogold for amplified optical detection of aflatoxin B1

Wang

Knopp

2015

Analyst

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A simple, sensitive and cost-effective method for the analysis of the mycotoxin aflatoxin B1 (AFB1) has been established based on controlled growth of immunogold. AFB1-BSA conjugate modified magnetic beads were employed as capture probe and anti-AFB1 antibody-coated gold colloids were used as detection probe for the immunological recognition of AFB1, as well as for signal transduction. The immune recognition event is converted into the gold enlargement signal which can be quantitatively measured by UV-vis spectroscopy. The autocatalytic enlargement of immunogold was conducted in aqueous solution containing chloroauric acid, hexadecyltrimethylammonium bromide and ascorbic acid. The reaction could be stopped by the addition of sodium thiosulfate. The final absorbance and resonance light scattering intensity were highly dependent on immunogold concentration. After gold enhancement, the sensitivity of the immunoassay was improved and total assay time reduced to 1 h. Under optimized conditions, the linear range and lower detection limit was 0.01-1 ng mL(-1) and 7 pg mL(-1), respectively. The proposed method offers great promise for sensitive detection of other mycotoxins and organic pollutants.

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Aflatoxin Measurement and Analysis

Cited by 20 publications

References 81 publications

Methods for Detection of Aflatoxins in Agricultural Food Crops

Methods for Detection of Aflatoxins in Agricultural Food Crops

Development of an aflatoxin B1 specific molecularly imprinted solid phase extraction sorbent for the selective pre-concentration of toxic aflatoxin B1 from child weaning food, Tsabana

Controlled growth of immunogold for amplified optical detection of aflatoxin B1

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