Aflatoxins levels in dried fruits and nuts from Rabat-Salé area, Morocco

Juan, Cristina; Zinedine, Abdellah; Moltó, J.C.; Idrissi, L.; Mañes, Jordi

doi:10.1016/j.foodcont.2007.08.010

Cited by 135 publications

(78 citation statements)

References 17 publications

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“…High moisture and temperature are two main factors that cause the occurrence of mycotoxins at pre-harvest and post harvest stages (26). Fungi are widely distributed as environmental contaminants (27), in fact, under favorable conditions of temperature and humidity, they grow on many commodities including cereals, oil seeds, nuts, herb-teas and spices (28). They have also been found in medicinal herbs that are not fully dried or stored improperly (e.g.…”

Section: Introductionmentioning

confidence: 99%

Determination of Aflatoxins in Medicinal Plants by High-Performance Liquid Chromatography–Tandem Mass Spectrometry

Mujeeb

Ahmad

et al. 2013

J Pharm Pharm Sci

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-Purpose. The intention of the proposed work is to study the presence of the aflatoxins B 1 , B 2 , G 1 and G 2 in medicinal plants, namely Mucuna pruriens, Delphinium denudatum and Portulaca oleraceae. Methodology. The aflatoxins were extracted, purified by immunoaffinity column chromatography and analysed by high-performance liquid chromatography-tandem quadrupole mass spectrometry with electrospray ionisation (HPLC-MS/MS). Fungal count was carried out in PDA media. Results. A good linear relationship was found for AFB 1 , AFB 2 , AFG 1 and AFG 2 at 1-10 ppb (r>0.9995). The analyte accuracy under three different spiking levels was 86.7-108.1 %, with low per cent relative standard deviations in each case. The aflatoxins can be separated within 5 to7 min using an Agilent XDB C18-column. We found that AFB 1 and AFB 2 were in trace amounts below the detection limit in M. pruriens whilst they were not detected in D. denudatum. P. oleraceae was found to be contaminated with AFB 1 and AFB 2 . AFG 1 and AFG 2 were not detected in M. pruriens, P. oleraceae and were below the detection limit in D. denudatum. This was consistent with very low numbers of fungal colonies observed after 6 hr of incubation. Conclusion. The analytical method developed is simple, precise, accurate, economical and can be effectively used to determine the aflatoxins in medicinal plants and therefore to control the quality of products. The aflatoxin levels in the plant extracts examined were related to the minimal fungal load in the medicinal plants examined.

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Section: Introductionmentioning

confidence: 99%

Determination of Aflatoxins in Medicinal Plants by High-Performance Liquid Chromatography–Tandem Mass Spectrometry

Mujeeb

Ahmad

et al. 2013

J Pharm Pharm Sci

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“…Some data on AF incidence in Africa (Table 2) presented low occurrence of the toxins in African fruits. Juan et al (2008) found AFB 1 at levels exceeding the EU limit of 2µg/kg in 5 and 20% of 20 samples each of pistachio and dried raisin, respectively, with the highest value of 1430 µg/kg detected in pistachio. While all dried fig samples were found fit for the EU market with regards to AFB 1 contents, 15% of them had AF levels above the 4 µg/kg, a maximum recommended limit of EU.…”

Section: Fruitsmentioning

confidence: 88%

Aflatoxin Contamination in Foods and Feeds: A Special Focus on Africa

Anthony¹,

Francis²,

Berka³

et al. 2012

Trends in Vital Food and Control Engineering

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“…Institute of Standards and Industrial Research of Iran (ISIRI) has set a MTL of 5 and 15 lg Kg -1 for AfB1 and total aflatoxins, respectively, in 2002. Among 20 types of aflatoxins, only aflatoxins B1, B2, G1 and G2 play a vital role in foods and feeds [7,8]. The simultaneous identification of total aflatoxins in a single test considerably reduces the time and costs of each analysis and is the most attractive approach practically.…”

Section: Introductionmentioning

confidence: 99%

Application of gold-labeled antibody biosensor in simultaneous determination of total aflatoxins using artificial neural network

Saidi

Mirzaei

2013

J IRAN CHEM SOC

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Preparation of antibody-coated gold nanoparticles (GNPs) specific to aflatoxins B1, B2, G1 and G2 and its use in developing aflatoxins diagnostic method were presented in this paper. The formation of gold-labeled antibodies was accomplished at optimal condition. Due to severe overlapping between the emission profiles for the aflatoxins, they cannot be determined by direct inspection of data. The strategy used in this study, constituted by artificial neural network (ANN), was easy to implement and to originate reliable results. ANN can be successfully applied to spectrofluorimetric spectra matrices to simultaneous determination of total aflatoxins. Quantitative results obtained using ANN method for aflatoxins in pistachio nuts samples were compared to those obtained using the HPLC method. Obtained results using these two methods did not show significant differences.

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Aflatoxins levels in dried fruits and nuts from Rabat-Salé area, Morocco

Cited by 135 publications

References 17 publications

Determination of Aflatoxins in Medicinal Plants by High-Performance Liquid Chromatography–Tandem Mass Spectrometry

Determination of Aflatoxins in Medicinal Plants by High-Performance Liquid Chromatography–Tandem Mass Spectrometry

Aflatoxin Contamination in Foods and Feeds: A Special Focus on Africa

Application of gold-labeled antibody biosensor in simultaneous determination of total aflatoxins using artificial neural network

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