AFLP markers for meat traceability of cattle in the Chinese market

Zhao, Jie; Li, Tingting; Xu, Zhenzhen; Wang, Zhiying; Yang, Shuming; Chen, Ailiang

doi:10.1016/j.foodcont.2018.04.022

Cited by 8 publications

(3 citation statements)

References 28 publications

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“…The resulting pattern is separated and visualized using Agarose gel electrophoresis. AFLP-PCR-based methods have been developed for the differentiation of tissue types from salmon and veal products [80], traceability of cattle [81], and olive oil [82,83].…”

Section: Genomics-based Methodsmentioning

confidence: 99%

Food authentication in real life: How to link nontargeted approaches with routine analytics?

Creydt¹,

Fischer²

2020

Electrophoresis

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In times of increasing globalization and the resulting complexity of trade flows, securing food quality is an increasing challenge. The development of analytical methods for checking the integrity and, thus, the safety of food is one of the central questions for actors from science, politics, and industry. Targeted methods, for the detection of a few selected analytes, still play the most important role in routine analysis. In the past 5 years, nontargeted methods that do not aim at individual analytes but on analyte profiles that are as comprehensive as possible have increasingly come into focus. Instead of investigating individual chemical structures, data patterns are collected, evaluated and, depending on the problem, fed into databases that can be used for further nontargeted approaches. Alternatively, individual markers can be extracted and transferred to targeted methods. Such an approach requires (i) the availability of authentic reference material, (ii) the corresponding highresolution laboratory infrastructure, and (iii) extensive expertise in processing and storing very large amounts of data. Probably due to the requirements mentioned above, only a few methods have really established themselves in routine analysis. This review article focuses on the establishment of nontargeted methods in routine laboratories. Challenges are summarized and possible solutions are presented.

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Section: Genomics-based Methodsmentioning

confidence: 99%

Food authentication in real life: How to link nontargeted approaches with routine analytics?

Creydt¹,

Fischer²

2020

Electrophoresis

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“…These markers bear significant variability in majority of loci. This feature enables the simultaneous detection of frequent variations of a single nucleotide concealed genomic regions (Zhao et al, 2018). This is widely used for the genetic relatedness of closely related animal groups and is effectively applied in the livestock industry for assessing the relationships between breeds as well as criminal studies and parentage determination.…”

Section: Aflp-amplified Fragment Length Polymorphismmentioning

confidence: 99%

Use of molecular biology techniques for animal identification and traceability

et al. 2022

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Animal husbandry is considered a substantial part of agriculture which is important for the economic development of a country. The local potential for the livestock sector has remarkably developed in the past few decades with the introduction of technological advancements. Identification and traceability of livestock populations evolved as critical points in the production chain. Molecular marker-based genetic studies have addressed the preliminary complications of the livestock field. The intended objective of genetic studies is to select and maintain superior traits which have a direct impact on economic value. In that regard, the application of genetic markers has a significant role. RFLP, SCAR, AFLP, SSR, ISSR, EST, RAPD, STR, SCoT, and STS markers are prominently used despite animal identification and traceability, determination of genetic distance, sex determination, parentage determination, genetic conservation, gene mapping approaches in the livestock field. The present review is a critical study of current molecular biological techniques and their versatile applications in animal identification and traceability approaches and possible improvements of the available techniques to address the major concerns in the field.

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“…AFLP analysis is a robust, multi-locus PCR-based DNA fingerprinting technique that can provide the most efficient, reliable, and economical population genetics analysis (El-Demerdash et al, 2019). AFLPs detect nuclear DNA markers inherited in a Mendelian fashion and provide a much greater level of polymorphism that cover a wider genomic area when compared to RAPD and ISSR (Zhao et al, 2018). Huda and coworkers (2019) reported that 90 million date palm trees are present in the world with an average life expectancy of 100 years each, and more than 70 per cent of these trees are grown in the Arab countries.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of Libyan date palms cultivars using amplified fragment length polymorphism and biochemical analysis

Abogmaza

Yahya²,

Misratia³

et al. 2022

JPC

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Phoenix dactylifera L. is a flowering plant species commonly known as the date palm and is widely cultivated in most Middle East countries, including Libya. The present study analysed the biochemical and genetic diversity of fully mature eight Libyan date palm cultivars grown in different regions using the amplified fragments length polymorphism (AFLP) technique. Six pairs of AFLP molecular marker combinations were utilised to discriminate the eight date palm genotypes. Fruit dimensions (length x diameter) varied based on the type; Majhool Alheelo fruit had the highest value (15.29 cm2), while the lowest value was for Alkhadraya fruits (7.9 cm2). Reducing sugar content ranged from 10.4 per cent of flesh dry weight in Umfetity cultivars to 61.2 per cent in Sufeer-genab, which also showed the highest polymorphism percentage (P%=4.9), while Alkhadarya was the lowest (P%=0.519). The phylogenetic tree indicated that the most distantly related cultivars were Sufeer-genab, Alhamraya and Majhool Alhelo. The two most closely related cultivars were the Alsaeedy Show and Alkhadarya, grown in different regions. Our results indicate that the nutritional and genetic diversity of Libyan cultivars is not closely matched with the growing region.

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AFLP markers for meat traceability of cattle in the Chinese market

Cited by 8 publications

References 28 publications

Food authentication in real life: How to link nontargeted approaches with routine analytics?

Food authentication in real life: How to link nontargeted approaches with routine analytics?

Use of molecular biology techniques for animal identification and traceability

Genetic diversity of Libyan date palms cultivars using amplified fragment length polymorphism and biochemical analysis

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