AG73-modified Bubble liposomes for targeted ultrasound imaging of tumor neovasculature

Negishi, Yoichi; Hamano, Nobuhito; Tsunoda, Yuka; Oda, Yusuke; Choijamts, Batsuren; Endo‐Takahashi, Yoko; Omata, Daiki; Suzuki, Ryo; Maruyama, Kazuo; Nomizu, Motoyoshi; Emoto, Makoto; Aramaki, Yukihiko

doi:10.1016/j.biomaterials.2012.09.056

Cited by 67 publications

(40 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…US imaging was performed as previously described [23]. Briefly, a dedicated small animal high spatial-resolution imagining linear transducer (16 MHz; gain, 25 dB; dynamic range, 50 dB) (Vevo 2100) was used.…”

Section: In Vivo Tumor Inhibition and Imagingmentioning

confidence: 99%

Evaluation of the potential of doxorubicin loaded microbubbles as a theranostic modality using a murine tumor model

et al. 2015

Self Cite

View full text Add to dashboard Cite

In this study, a novel phospholipid-based microbubble formulation containing doxorubicin and perfluoropropane gas (DLMB) was developed. The DLMBs were prepared by mechanical agitation of a phospholipid dispersion in the presence of perfluoropropane (PFP)gas. An anionic phospholipid, distearoylphosphatidylglycerol (DSPG) was selected to load doxorubicin in the microbubbles by means of electrostatic interaction. The particle size, zeta potential, echogenicity and stability of the DLMBs were measured. Drug loading was ≥92%.The potential of the DLMBs for use as a theranostic modality were evaluated in tumor bearing mice. Gas chromatography analysis of PFP showed significant enhancement of PFP retention when doxorubicin was used at concentrations of 10-82% equivalent to DSPG. The inhibitory effects on the proliferation of B16BL6 melanoma murine cells in vitro were enhanced using a combination of ultrasound (US) irradiation and DLMBs. Moreover, in vivo DLMBs in combination with (US) irradiation significantly inhibited the growth of B16BL6 melanoma tumor in mice. Additionally, US echo imaging showed high contrast enhancement of the DLMBs in the tumor vasculature. These results suggest that DLMBs could serve as US triggered carriers of doxorubicin as well as tumor imaging agents in cancer therapy.

show abstract

Section: In Vivo Tumor Inhibition and Imagingmentioning

confidence: 99%

Evaluation of the potential of doxorubicin loaded microbubbles as a theranostic modality using a murine tumor model

et al. 2015

Self Cite

View full text Add to dashboard Cite

show abstract

“…Complexes that strongly bind to nucleic acids are able to permeate the nuclear membrane. For example, complexes [Ir(N^C) 2 (N^N)] + (12)(13)(14)(15)(16)(17) efficiently enter the nuclei of Madin-Darby canine kidney (MDCK) cells, and are then extracted to hydrophobic pockets of nucleolar proteins [18]. Since the fixed cells show the same staining pattern, the accumulation of the complexes in the nucleoli is due to the affinity of the complexes to cellular structures, rather than a consequence of active sequestration by the live cells.…”

Section: (N^n)]mentioning

confidence: 93%

Phosphorescent Iridium(III) Complexes for Bioimaging

Zhang

Liu

Zhao

et al. 2014

Luminescent and Photoactive Transition Metal Complexes as Biomolecular Probes and Cellular Reagents

View full text Add to dashboard Cite

Phosphorescent iridium(III) complexes have received increasing attention in bioimaging applications owing to their advantageous photophysical properties and efficient internalization into live cells. In this chapter, we summarize the recent design of bioimaging reagents based on phosphorescent iridium(III) complexes. The utilizations of cationic, neutral, and zwitterionic phosphorescent iridium(III) complexes in bioimaging applications have been described first.Complexes showing aggregation-induced phosphorescence have also been included considering the absence of the commonly observed aggregation-caused quenching. Then we discuss the functionalization of iridium(III) complexes with biological substrates and reactive groups, which allows non-covalent and covalent interaction, respectively, with intracellular biomolecules. As the photophysical properties of iridium(III) complexes are very sensitive toward their surrounding ligands and microenvironment, the use of these complexes as intracellular sensors for gas molecules, ions, and amino acids has been summarized. Additionally, the incorporation of iridium(III) complexes into dendrimer, polymer, and nanoparticle systems providing attractive functionalities has been discussed. Furthermore, various strategies, including the use of near-infrared-emitting and two-photon excitable complexes, upconversion nanoparticles, and lifetime-based microscopy techniques, to enhance signal-to-noise ratios in bioimaging have been discussed. At last, the design of reagents for multi-mode imaging techniques involving phosphorescence and magnetic resonance imaging has been described.

show abstract

“…Fig.1 (a) and (b) showed that BLs are smaller than MBs. The diameter of most BLs is less than 2µm, and the average diameter is about 500 nm [10]. The suspension of BLs was prepared just before the experiment at a concentration of 0.01-0.05 mg lipid/ml, which was diluted with a saline.…”

Section: A Bubble Liposemesmentioning

confidence: 99%

Active control of bubble liposome through artificial capillary by using matrix array transducer

Koda

Hosaka

Onogi

et al. 2013

2013 IEEE International Ultrasonics Symposium (IUS)

Self Cite

View full text Add to dashboard Cite

We have ever reported our attempts to control microbubbles (MBs) using the primary and secondary acoustic force for active control in artificial blood vessels. Recently, we have demonstrated active path selection of MBs by using a matrix array transducer to produce multiple focal points. On the other hand, bubble liposomes (BLs) have an advantage in easily modifying targeting ligand. However, considering that BLs are several hundred nanometers in diameter, there were some difficulties in controllability of bubbles in blood flow under ultrasound exposure, since acoustic forces are less affected to these small bubbles. In this study, we used the liposomes (BLs) entrapping perfluoropropane gas with the average diameter of 500 nm and applied it to control the behavior in an artificial blood vessel. First, we observed aggregates formation of BLs in static water by secondary acoustic force. Next, we investigated the BLs control in multi-bifurcated flow by using a matrix array transducer. As a result, the streaming of BLs was viscously propelled to a desired path. BLs are much tied each other because of surface interaction of the lipid membrane and then caused a resistance to flow compare to MBs.

show abstract

AG73-modified Bubble liposomes for targeted ultrasound imaging of tumor neovasculature

Cited by 67 publications

References 39 publications

Evaluation of the potential of doxorubicin loaded microbubbles as a theranostic modality using a murine tumor model

Evaluation of the potential of doxorubicin loaded microbubbles as a theranostic modality using a murine tumor model

Phosphorescent Iridium(III) Complexes for Bioimaging

Active control of bubble liposome through artificial capillary by using matrix array transducer

Contact Info

Product

Resources

About