: The present study aimed to estimate the age, gender, and height of the Iranian population based on the characteristics of the 10th and 11th thoracic vertebrae for identification. This cross-sectional study measured the T10 and T11 indices in axial, sagittal, and coronal computed tomography (CT) scan images. The measured indices included vertebral length (VL), endplate depth (EPD), endplate width (EPW), foramen diameter (FD), foramen width (FW), transverse process distance (TDm), spinal process height (SPH), spinal process length (SPL), vertebral body height (VBH), pedicle height (PH), pedicle width (PW), articular process height inferior (APHi), articular process height superior (APHs), and maximum distance between articular processes (ADm). The mean differences of the T10 and T11 indices between the two groups of men and women, except FW, FD, and APHi, were higher in men, which were statistically significant (P < 0.006). VL, EPD, EPW, FW, TDm, VBH, PH, and ADm in men, and VL, EPD, EPW, TDm, SPH, SPL, PH, APHs, and ADm in women were significantly correlated with height in the T10 vertebra (P < 0.05). Moreover, FD in T10 was found to be negatively correlated with women’s height (P = 0.04). In addition, in the T11, the VL, EPD, EPW, FW, FD, TDm, and VBH indices in men and the VL, EPW, TDm, SPL, PH, APHs, and PW in women were significantly correlated with height (P < 0.05). Moreover, VL, EPD, TDm, SPH, SPL, and APHi in women and VL in men were significantly and positively correlated with age in T10 (P < 0.05), similar to the T11 APHs correlation with age in men and VL, EPW, EPD, SPL, and APHi in women. The current study showed that the T10 and L11 vertebral indices were significantly correlated with gender, height, and age.