Age dependency of high-affinity ouabain receptors and their magnetosensitivity

Abstract

Age dependency of [ 3 H]-ouabain binding, 45 Ca 2? eflux and its magnetosensitivity in rats' brain cortex and heart muscle tissues were studied. Curves of dose-dependent [ 3 H]-ouabain binding consisted of three components with different affinities (10 -7 -10 -4 M (a 1 ); 10 -9 -10 -7 M (a 2 ); and 10 -11 -10 -9 M (a 3 )). These curves were also characterized by different dose-dependent kinetics. [ 3 H]-ouabain binding with a 3 receptors in brain cortex and heart muscle tissues of young and adult animals had a dose-dependent character, while that in old ones had a doseindependent character. A 0.2 T static magnetic field (SMF) exposure had modulation effect on ouabain binding with a 1 , a 2 and a 3 receptors in young rats, while in adult ones, only a 3 receptors were magnetosensitive. In old animals, SMF exposure had no significant effect on ouabain binding with a 3 receptors in brain cortex, while in heart muscle, it had inhibitory effect on it. Age-dependent effect of ouabain impact on 45 Ca 2? efflux showed that all concentrations of ouabain lead to inhibitory effect in young animals' brain cortex and heart muscle (with the exception of 10 -10 and 10 -6 M), while in old ones, it had activation effect as compared with data received without ouabain. SMF exposure in young animals had activation effect on 45 Ca 2? efflux from brain cortex and heart muscle in data without ouabain, and in old rats, 45 Ca 2? efflux from brain cortex was magnetic insensitive. In old animals, SMF increased 45 Ca 2? efflux even after extra low concentration of ouabain. It is suggested that a 3 receptors having a crucial role in the regulation of Na ? /Ca 2? exchange serve as age-dependent magnetosensors of excitable cells.

Age-Dependent Brain Tissue Hydration, Ca Exchange and their Dose- Dependent Ouabain Sensitivity

“…They also have a different localization in cells: α 1 is ubiquitously distributed over the surfaces of cells, while high ouabain affinity isoforms are confined to a reticular distribution within the cellular membrane that paralleled underlying endoplasmic or sarcoplasmic reticulum, with Na + /Ca 2+ exchanger protein. The expression of these isoforms is age-dependent [20][21][22][23][24][25]. While the role of low-affinity receptors as a working molecule for Na + /K + pump is well established [25][26][27], the functional role of high-affinity receptors is still discussable [13].…”

The role of cell hydration in realization of biological effects of non-ionizing radiation (NIR)

“…In the present series of experiments, the comparative study of the effects of direct NIR exposure on animals and magnetized PS on cell proliferation and hydration, 3 H-ouabian binding with cell membrane, 45 Ca 2+ uptake and intracellular cyclic nucleotides contents in different tissues of rats was performed Gharibova et al, 1996;Heqimyan et al, 2012;Narinyan et al, 2012Narinyan et al, , 2013. It is known that the deoxyribonucleic acid (DNA) hydration has a key role in regulation of its activity (Chaplin, 2006).…”

The Skeletal Muscle Impedancemetric Characteristics as a Marker for Detection of Functional State of Organism

“…It is known that any cell pathology (including cancer, nerve, cardiovascular diseases, and aging) is a consequence of dysfunction of metabolic controlling of cell hydration [1,[12][13][14]. As can be seen in Table 1 the sarcoma-180 injection leads to overhydration in different tissues (heart muscle, skeletal muscle, lung, liver, spleen, and kidney).…”

“…As can be seen from data presented in Table 1 cisPt intraperitoneal injection had also pronounced hydration effect on skeletal muscle (30.5%) and dehydration effect on lung (45%). The fact that NA + /K + pump has a crucial role in cell hydration and its dysfunction can be considered as a common consequence of any pathology [1,[12][13][14]. The effect of ouabain (specific inhibitor for NA + /K + pump) on various tissues' hydration was studied.…”