Age-dependent changes in the in-vitro response of a pig Sertoli cell-enriched population to FSH

Monet-Kuntz, C.; Fontaine, I.

doi:10.1530/jrf.0.0860643

Cited by 8 publications

(6 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is therefore assumed that both the indirect effect of the low LH secretion by the pituitary and the direct effect of the low T secretion by the contralateral testis induced by the high plasma E 2 levels caused the steroidogenic and spermatogenic dysfunctions in the contralateral testis. Sertoli cell function in the testis is controlled by follicle stimulating hormone (FSH) secreted by the anterior pituitary [20]. Testicular concentrations of Tf produced by Sertoli cells have been found to reflect Sertoli cell function [21].…”

Section: Discussionmentioning

confidence: 99%

Function of Contralateral Testis after Artificial Unilateral Cryptorchidism in Dogs

Kawakami

Hori

Tsutsui

1999

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

ABSTRACT. The effects of a cryptorchid testis on the contralateral testis were investigated after artificially producing unilateral cryptorchidism in 8 beagle dogs. Bilateral testicular biopsy and collection of spermatic vein blood and peripheral vein blood were performed at the time of the operation to produce the cryptorchidism and 52 weeks later. The testicular tissue was used for histological examination by light microscopy and measurement of the testicular transferrin (Tf) concentration by enzyme immunoassay. Plasma testosterone (T), estradiol-17β (E 2 ), and luteinizing hormone (LH) levels were measured by radioimmunoassay. Semen was collected weekly and its quality was examined. No spermatogenesis was observed in the cryptorchid testes at 52 weeks after the operation, and the number of germ cells in the contralateral testes had decreased but the number of Sertoli cells did not change. The Tf concentration in both testes had also decreased. The mean total number of sperm between 48 and 52 weeks after the operation (194 × 10 6 ) was less than half the number before the operation (510 × 10 6 ). Mean spermatic vein plasma T levels (51 ng/ml) in the cryptorchid testes 52 weeks after the cryptorchid operation were significantly lower than before the operation (91 ng/ml; P<0.05). By contrast, spermatic vein plasma E 2 levels (80 pg/ml) were significantly higher than the values before the operation (51 pg/ml P<0.05). The peripheral plasma LH levels decreased. These findings indicate that a large quantity of E 2 secreted by the cryptorchid testis inhibits the endocrine and spermatogenic functions of the contralateral testis in the dog. In particular, it is assumed that dysfunction of the contralateral testis is associated with Sertoli cell dysfunction suggested by the low Tf concentration.-KEY WORDS: artificial cryptorchidism, canine, estrogen.

show abstract

Section: Discussionmentioning

confidence: 99%

Function of Contralateral Testis after Artificial Unilateral Cryptorchidism in Dogs

Kawakami

Hori

Tsutsui

1999

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

show abstract

“…Testosterone secreted by Leydig cells is known to stimulate spermatogenesis, through a role for FSH is also established ( Sharpe, 1994). FSH regulates Sertoli cell function ( Monet‐Kuntz & Fontaine, 1989; Skinner & Griswold, 1982), and Sertoli cell function is presumed to be reflected by testicular Tf concentrations ( Nambu & Kumamoto, 1995). Decreased numbers of Sertoli cells and Sertoli cell dysfunction have been reported to cause low spermatogenic function in humans ( Holmes et al ., 1982 ; Meyer et al ., 1996 ) and stallions ( Blanchard & Johnson, 1997).…”

Section: Discussionmentioning

confidence: 99%

Changes in testicular transferrin concentrations measured by enzyme immunoassay after FSH administration to beagle dogs

1999

View full text Add to dashboard Cite

A sensitive enzyme immunoassay (EIA) was developed for transferrin (Tf) in the canine testis, as an effective marker of Sertoli cell function. Tf was purified from male dog serum by DEAE-Affi-Gel Blue chromatography and gel filtration. Rabbits were immunized with the Tf, and anti-dog Tf antibody was purified from the rabbit serum by affinity chromatography as primary antibody for EIA. Peroxidase-conjugated goat anti-dog Tf antibody was used as the secondary antibody for microtitre plate double-antibody EIA. The EIA was applied to measurement of testicular Tf concentrations in five normal beagle dogs. All of the dogs were given five injections of 2 mg porcine pituitary FSH at 12 h intervals, and testicular biopsy was performed. The testicular tissue was used for EIA of Tf. The optimum dilutions of the primary and secondary antibodies for the standard curves of canine Tf were 1: 4000 and 1: 1000, respectively. The limits of sensitivity of our EIA were between 30 ng Tf/plate-well and 2500 ng/well. The intra- and inter-assay coefficients of variation, and the recovery rate were 8.6, 7.6 and 86.0%, respectively. Before FSH administration, the mean +/- SEM Tf concentration in the testis of normal dogs was 357 +/- 31 ng/mL. At 12 h after the final FSH administration, Tf concentrations were significantly increased (p < 0.5) when compared with values before the start of treatment. Thus, Tf in the canine testis, measured by our EIA, appears capable of serving as a marker of Sertoli cell function.

show abstract

“…Harrop [3] reported failing to find any changes in the semen quality of dogs with spermatogenic dysfunction after LH and FSH treatment [3]. As it is well-known that spermatogenesis is maintained by T [17] and FSH [13], in the present study changes in spermatogenesis and the semen quality of three dogs with asthenozoospermia, in which GnRH-analogue or hCG therapies proved ineffectual, were investigated after a treatment of T plus pregnant mare serum gonadotropin (PMSG), which has FSH-like activity in dogs with spermatogenic dysfunction.The three dogs treated with T-depot plus PMSG were two Beagle dogs (dogs No. 1 and No.…”

mentioning

confidence: 99%

“…T secreted by Leydig cells is known to stimulate the latter half of spermatogenesis or spermiogenesis in the seminiferous tubules [17], and thus the number of round spermatids is presumed to have significantly increased in response to treatment with exogenous T. Oral treatment of T enanthate has been reported to result in increased sperm counts and sperm motility in humans [15,16], and the T enanthate in the present study is believed to have transiently improved the semen quality of the three dogs by stimulating spermatogenic function and epididymal and prostatic function. FSH secreted by the pituitary is known to control Sertoli cell function [13], and the testicular Tf concentration is an accepted indicator of the functional state of Sertoli cells [14]. PMSG is also thought to have improved spermatogenic function through its action on Sertoli cells, e.g., by stimulating Tf secretion, by its FSH-like activity.…”

mentioning

confidence: 99%

Changes in Plasma Testosterone and Testicular Transferrin Concentration, Testicular Histology and Semen Quality after Treatment of Testosterone-Depot plus PMSG to 3 Dogs with Asthenozoospermia.

Kawakami

Hori

Tsutsui

2000

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

Three dogs diagnosed as having asthenozoospermia were given three intramuscular injections of 50 mg testosterone(T)-depot plus 250 IU pregnant mare serum gonadotropin (PMSG) at 2-week intervals, and their plasma T and testicular transferrin (Tf) concentrations, testicular histology, and semen quality were examined during the period of hormone therapy. Plasma T concentrations temporarily increased, and there was a slight improvement in spermatogenesis. Increased Tf concentrations suggested that Sertoli cell function in all three dogs was promoted by hormone treatment. The results showed that semen quality, especially the percentages of motile sperm and abnormal sperm, were improved between 1 and 5 weeks after the start of T-depot plus PMSG treatment.-KEY WORDS: asthenozoospermia, canine, testosterone plus PMSG treatment. J. Vet. Med. Sci. 62(2): 203-206, 2000 intervals. Peripheral blood samples were collected 4 times a day at-7,-1, 1, 3, 7, and 14 days after the start of hormone treatment. Plasma T concentrations were measured by radioimmunoassay, as reported previously [7]. The right testis and the left testis were biopsed under halothane inhalation anesthesia 1 week before and 5 weeks after the start of hormone treatment, respectively. The testicular tissue collected was divided into two portions, with one portion being used to measure testicular transferrin (Tf) concentrations by our EIA [6], and the other portion being used for histological examination after staining with PAS-hematoxylin. Germ cell counting was performed by the methods described previously [10] in 5 seminiferous tubules at stage 9 of the seminiferous epithelial cycle. Semen specimens were collected by digital manipulation at one-week intervals and each specimen was evaluated by the methods described previously [9]. Plasma T concentrations in the three dogs peaked on Day 1 after the start of hormone treatment (7.8-12.0 ng/ml), and then decreased to the basal levels (1.4-2.0 ng/ml) on Day 14. The testicular Tf concentrations in all three dogs clearly increased (Table 1) after T-depot plus PMSG treatment, and spermatogenesis in the seminiferous tubules improved (Table 1 and Fig. 2). Semen quality improved between 1 and 5 weeks after the start of T-depot plus PMSG treatment (Fig. 1). It was of particular interest that the number of sperm and the percentages of motile sperm increased every week after each of the three hormone treatments in all three dogs. The percentages of motile sperm in Dogs No. 1 and No. 2 increased from below 50% before hormone treatment to 80% between 3 weeks and 5 weeks after the start of hormone treatment. The percentages of morphologically abnormal sperm, mainly bent or coiled tail, were transiently decreased by this hormone therapy. The plasma T concentration of normal dogs has been The authors have used GnRH-analogue or hCG to treat dogs with low plasma testosterone (T) concentrations and spermatogenic dysfunction, but the semen quality of a few of the treated dogs did not improve at all [7, 8]. Harrop [3] reported fail...

show abstract

Age-dependent changes in the in-vitro response of a pig Sertoli cell-enriched population to FSH

Abstract: Summary. The

Cited by 8 publications

References 8 publications

Function of Contralateral Testis after Artificial Unilateral Cryptorchidism in Dogs

Function of Contralateral Testis after Artificial Unilateral Cryptorchidism in Dogs

Changes in testicular transferrin concentrations measured by enzyme immunoassay after FSH administration to beagle dogs

Changes in Plasma Testosterone and Testicular Transferrin Concentration, Testicular Histology and Semen Quality after Treatment of Testosterone-Depot plus PMSG to 3 Dogs with Asthenozoospermia.

Contact Info

Product

Resources

About