2009
DOI: 10.1152/ajplung.00168.2009
|View full text |Cite
|
Sign up to set email alerts
|

Age-dependent endothelial nitric oxide synthase uncoupling in pulmonary arteries of endoglin heterozygous mice

Abstract: , and clinical signs of disease are generally more evident later in life. We previously showed that systemic vessels of adult Eng heterozygous (Eng ϩ/Ϫ ) mice exhibit increased vasorelaxation due to uncoupling of endothelial nitric oxide synthase (eNOS). We postulated that these changes may develop with age and evaluated pulmonary arteries from newborn and adult Eng ϩ/Ϫ mice for eNOS-dependent, acetylcholine (ACh-induced) vasorelaxation, compared with that of age-matched littermate controls. While ACh-induced … Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

2
28
0

Year Published

2010
2010
2023
2023

Publication Types

Select...
7

Relationship

0
7

Authors

Journals

citations
Cited by 33 publications
(30 citation statements)
references
References 47 publications
2
28
0
Order By: Relevance
“…Superoxide may enhance pulmonary vascular tone in rats via its direct stimulation of ROCK, thus promoting inhibition of myosin lightchain phosphatase activity and calcium sensitization (15). We have previously shown that H 2 O 2 is a pulmonary vasodilator in mice (22) and have documented the same response in the pulmonary arteries of newborn rats in this study. Therefore, we speculate that lung SOD content and activity may modulate the fate of hyperoxia-induced superoxide formation thereby favoring a reduction or an increase in pulmonary arterial response to thromboxane.…”
Section: Oxygen and Newborn Pulmonary Vasculaturesupporting
confidence: 75%
See 1 more Smart Citation
“…Superoxide may enhance pulmonary vascular tone in rats via its direct stimulation of ROCK, thus promoting inhibition of myosin lightchain phosphatase activity and calcium sensitization (15). We have previously shown that H 2 O 2 is a pulmonary vasodilator in mice (22) and have documented the same response in the pulmonary arteries of newborn rats in this study. Therefore, we speculate that lung SOD content and activity may modulate the fate of hyperoxia-induced superoxide formation thereby favoring a reduction or an increase in pulmonary arterial response to thromboxane.…”
Section: Oxygen and Newborn Pulmonary Vasculaturesupporting
confidence: 75%
“…The functional in vitro evaluation of newborn pulmonary arteries has been previously described (38). Briefly, third-generation lung intralobar pulmonary artery ring segments (average diameter 80-100 μm and length 2 mm) were dissected free and mounted in a wire myograph (Danish Myo Technology A/S, Aarhus, Denmark).…”
Section: Organ Bath Studiesmentioning
confidence: 99%
“…[28][29][30][31] . Attention now focuses on aberrant vascular responses to injury-induced angiogenic stimuli, when the mutated genes in HHT appear to result in the inability of a blood vessel to mature appropriately.…”
Section: Overview Of Hhtmentioning
confidence: 99%
“…160,168 Endogenous Smad phosphorylation in mural cells is reduced, 168 29,173 Support for a fundamental role for aberrant angiogenesis and reactive oxygen species in HHT disease pathogenesis is accumulating in man, with case reports and small series suggesting that anti-angiogenic and anti-oxidant strategies may be of therapeutic benefit in HHT (see Sections 4 and 5 below).…”
Section: 2d) Generation Of Abnormal Vessels In Hhtmentioning
confidence: 99%
“…28 Endoglin is a transforming growth factor-β coreceptor highly expressed in endothelial cells and is associated with LV filling pressures and LV dysfunction. 29 Like sFlt-1, endoglin has been shown to regulate the expression and activity of endothelial NO synthase, 30 reducing NO production 31 and making it an important modulator of endothelial vasomotor tone. 32 Endothelial dysfunction in the acute setting may also contribute to coronary ischemia and subsequent arrhythmia.…”
mentioning
confidence: 99%