Age-related M1/M2 phenotype changes in circulating monocytes from healthy/unhealthy individuals

Abstract

Macrophage polarization is a candidate biomarker of disease-related inflammatory status, but its modulation during aging has not been investigated. To do this, the M1/M2 profile was assessed by CD80/CD163 gating in classical (CD14++CD16-), intermediate (CD14++CD16+), and non-classical (CD14lowCD16+) monocytes from 31 healthy subjects (CTRs) of different ages. Cytofluorimetric analysis showed a significantly different CD80/CD163 distribution in the three subsets, as more than 80% of classical and intermediate monocytes were CD80+CD163+, whereas most non-classical monocytes were CD80-CD163- and CD163+. Non-classical CD163+ monocytes were significantly higher whereas classical CD163+ and CD80-CD163- monocytes significantly lower in older than younger CTRs (cut-off, 65 years), suggesting different age-related trends for M2 subsets. To establish whether an M1/M2 imbalance could be associated with disease, 21 patients with acute myocardial infarction (AMI) were compared with older CTRs. The AMI patients showed a significantly decreased proportion of CD163+CD80+ and an increased proportion of CD163+ and CD163-CD80- cells among classical monocytes, opposite trends to those observed in healthy aging. Moreover, a significantly greater proportion of intermediate and non-classical CD80+ monocytes suggested a shift to a pro-inflammatory phenotype. Overall, CD163/CD80 cytofluorimetric characterization of circulating monocytes provides additional information about their polarization and could be an innovative tool to monitor aging.

Peripheral blood CD163(+) monocytes and soluble CD163 in dry and neovascular age‐related macular degeneration

“…Flow cytometric analysis of peripheral blood immune cells is an established procedure that can be repeated longitudinally in individuals. An altered CD163(+) peripheral blood monocyte level was reported in acute myocardial infarction 38 . Our results showed intriguing differences between normal age‐matched control subjects and AMD patients in the percentages of the CD163(+) cells and serum sCD163.…”

Plasma-Derived Reactive Species Shape a Differentiation Profile in Human Monocytes

“…These factors could be responsible for the slightly different ROS-induced differentiation patterns observed in both cell types, despite the fact that the treatment varied by a factor of 6 to 12, owing to the increased robustness of THP-1 cells towards ROS-induced cell death [67]. In primary monocytes, the authors observed an increase of CD80 being a stimulatory receptor for T cell activation [68] and CD163, a receptor for the hemoglobin-haptoglobin complex and M2 macrophage marker [68] with plasma treatment. The mannose receptor and M2 macrophage marker CD206 was not changed in plasma treated primary monocytes, partly arguing against such M2 phenotype upon ROS-induced differentiation.…”

Quantification of aminobutyric acids and their clinical applications as biomarkers for osteoporosis

“…Correlation analysis for aminobutyric acid related genes. Since osteoporosis is a metabolic syndrome, where inflammation plays a major role, and given the relevance of peripheral blood monocytes (PBM) to the pathogenesis of aging-related, inflammatory, and degenerative diseases [27][28][29] , we investigated the gene expression profiles for the aminobutyric acids-related genes from PBMs. PBM information was available from 122 out of 136 young women subjects with high or low BMD (Supplementary Table 7) 30 .…”