Agents Protecting against Sepsis from the Roots of Angelica dahurica

Song, Dong‐Keun; Kim, Jiyeon; Li, Gao; Lee, Kyeung-Seon; Seo, Chang‐Seob; Yan, Ji-Jing; Jung, Jun‐Sub; Kim, Hyojin; Chang, Hyun-Wook; Son, Jong‐Keun

doi:10.1248/bpb.28.380

Cited by 15 publications

(13 citation statements)

References 19 publications

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“…Recently, we isolated preventive agents against sepsis from EtOAC extract of the roots of A. dahurica (Song et al, 2005), and as part of our continuing efforts to find a new class of COX-2/5-LOX dual inhibitors produced by this plant, we successfully isolated isoimperatorin (4-[(3-Methyl-2-butenyl)oxy]-7H-furo[3,2-g][1]benzopyran-7-one), which we evaluated in this study. The cytotoxicity of isoimperatorin (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The isolated compound was chemically and structurally identified using previously described methods (Song et al, 2005), and its chemical structure is shown in Fig. 1.…”

Section: Methodsmentioning

confidence: 99%

“…(Umbelliferae) have been used as an antipyretic and analgesic for the treatment of colds, headaches and toothaches in Korean and Chinese folk medicine. The biological activities of components from this plant, such as hepatoprotective activity on tacrine-induced cytotoxicity in Hep G2 cells, inhibition of compound 48/80-induced histamine release in mouse peritoneal cavity, antimicrobial activity, affinity to brain benzodiazepine receptor in vitro, antioxidant activity, anti-septic activity and acetylcholinesterase inhibitory activity have been reported (Bergendorff et al, 1997;Kimura et al, 1997;Kwon et al, 1997;Oh et al, 2002;Piao et al, 2004;Song et al, 2005). In addition, it was recently reported that byakangelicol isolated from this plant showed COX-2 inhibitory activity (Lin et al, 2002).…”

Section: Introductionmentioning

confidence: 92%

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The effects of isoimperatorin isolated from Agelicae dahuricae on cyclooxygenase-2 and 5-lipoxygenase in mouse bone marrow-derived mast cells

et al. 2008

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Isoimperatorin (4-[(3-Methyl-2-butenyl)oxy]-7H-furo[3,2-g][1]benzopyran-7-one) is a medicinal herbal product that is isolated from the dried roots of Angelicae dahuricae. Isoimperatorin inhibits the cyclooxygenase-2 (COX-2) and COX-1-dependent phases of prostaglandin D2 (PGD2) generation in bone marrow-derived mast cells (BMMC) in a concentration-dependent manner, with IC50 values of 10.7 microM and 24 microM, respectively. However, this compound was not able to inhibit COX-1 and 2 protein expression in BMMC that were treated with concentrations of up to 50 microM, which indicates that isoimperatorin directly inhibits COX-2 activity. Furthermore, this compound consistently inhibited the production of leukotriene C4 (LTC4), as well as the degranulation reaction in BMMC, with an IC50 value of 5.7 microM and 9 microM, respectively, and these effects occurred in a dose dependent fashion. These results demonstrate that isoimperatorin has a dual cyclooxygenase-2 selective/5-lipoxygenase inhibitory activity, and therefore may provide the basis for novel anti-inflammatory drugs.

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Section: Resultsmentioning

confidence: 99%

“…The isolated compound was chemically and structurally identified using previously described methods (Song et al, 2005), and its chemical structure is shown in Fig. 1.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 92%

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The effects of isoimperatorin isolated from Agelicae dahuricae on cyclooxygenase-2 and 5-lipoxygenase in mouse bone marrow-derived mast cells

et al. 2008

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“…Only 3 patients showed a response; 1 with renal cell carcinoma, 1 with ovarian carcinoma and 1 with malignant melanoma. The most important side effects were nausea/vomiting, myelosuppression and renal toxicity; 1 patient died of renal failure [25]. …”

Section: Experience With Single-agent Therapymentioning

confidence: 99%

Anticancer Effects of Imperatorin Isolated from Angelica dahurica: Induction of Apoptosis in HepG2 Cells through both Death-Receptor- and Mitochondria-Mediated Pathways

Luo¹,

Sun²,

Chan³

et al. 2011

Chemotherapy

127

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Background: Imperatorin (IM) is a furanocoumarin isolated from the root of Angelica dahurica, which is reported to have anticonvulsant and anticancer effects. In this study, the antiproliferative effect of IM on 9 human cancer cell lines was examined, and human hepatoma HepG2 cells were chosen as the target for preferential killing by IM. Particularly, the mechanism of IM-induced apoptosis and in vivo animal effects were also studied. Methods: Cell viability was measured using MTT assay, and apoptosis was detected by Hoechst staining, annexin V-PI staining, and DNA laddering assay. Mitochondrial membrane potential was detected by JC-1 staining. Western blot analysis was employed to detect the expression of apoptosis-related proteins. In addition, the in vivo anticancer effect of IM was examined in nude mice bearing HepG2 cells. Results: IM inhibited the proliferation of HepG2 cells through apoptosis induction in a time- and dose-dependent manner by observation of the nuclear morphology, DNA fragmentation, phosphatidylserine externalization, loss of mitochondrial membrane potential, release of cytochrome c into cytosol, and activation of caspase-3, caspase-8, caspase-9, and poly(ADP-ribose) polymerase cleavage. As cell death could partly be prevented by the caspase-8 or caspase-9 inhibitor and was evidenced by the results of Western blot analysis, our results also suggest that IM-induced apoptosis is mediated through both death receptor and mitochondrial pathways. In the animal model, IM was found to effectively suppress tumor growth by 31.93 and 63.18% at dosages of 50 and 100 mg/kg, respectively, after treatment for 14 days. No significant weight loss or toxicity to the hosts was found. Conclusions: IM can function as a cancer suppressor by inducing apoptosis in HepG2 cells through both death-receptor- and mitochondria-mediated pathways. Furthermore, the in vivo antitumor activities of IM are significant with negligible weight loss and damage to the host.

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“…ADR contains effective components such as coumarins and terpenoid (Kim et al, 1998;Joo and Kang, 2005). Its some coumarins on ADR are reported to have anti-microbial, oxidative, and thrombotic activity Kwon et al, 1997;Ryu et al, 2001;Lechner et al, 2004, Song et al, 2005. Meanwhile, inflammation is associated with the various diseases and other studies on anti-inflammatory activity have reported in medical plants (Shin et al, 1988;Son et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Inhibitory Effects of Angelicae Dahuricae Radix Extract on COPD induced by Cigarette Smoke Condensate and Lipopolysaccharide in Mice

Kwak¹,

Lim²

2011

Korean Journal of Medicinal Crop Science

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Agents Protecting against Sepsis from the Roots of Angelica dahurica

Abstract: In the course of isolating agents preventing sepsis from the EtOAc extract of the roots of Angelica dahurica, four known furanocoumarins, isoimperatorin (1), oxypeucedanin (2)

Cited by 15 publications

References 19 publications

The effects of isoimperatorin isolated from Agelicae dahuricae on cyclooxygenase-2 and 5-lipoxygenase in mouse bone marrow-derived mast cells

The effects of isoimperatorin isolated from Agelicae dahuricae on cyclooxygenase-2 and 5-lipoxygenase in mouse bone marrow-derived mast cells

Anticancer Effects of Imperatorin Isolated from Angelica dahurica: Induction of Apoptosis in HepG2 Cells through both Death-Receptor- and Mitochondria-Mediated Pathways

Inhibitory Effects of Angelicae Dahuricae Radix Extract on COPD induced by Cigarette Smoke Condensate and Lipopolysaccharide in Mice

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