AGER promotes proliferation and migration in cervical cancer

Zhu, Xuejie; Zhou, Lulu; Li, Ruyi; Shen, Qi; Cheng, Hui; Shen, Zhi‐Liang; Zhu, Haiyan

doi:10.1042/bsr20171329

Cited by 16 publications

(14 citation statements)

References 29 publications

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“…As indicated by global cancer statistics, cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC) ranks as the fourth most common cause of cancer-related mortality in women (1,2). On average, the 5-year survival rate of patients with CESC is 17% (3). Human papillomavirus (HPV) infection is considered the leading cause of CESC (4).…”

Section: Introductionmentioning

confidence: 99%

Upregulation of CCT3 promotes cervical cancer progression through FN1

Dou

Zhang

2021

Mol Med Rep

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Section: Introductionmentioning

confidence: 99%

Upregulation of CCT3 promotes cervical cancer progression through FN1

Dou

Zhang

2021

Mol Med Rep

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“…As one of the fatal malignant neoplasms, the morbidity of cervical carcinoma remains has drastically increased worldwide. After cervical carcinoma cell diffuses to secondary tissues, cervical carcinoma becomes resistant to the conventional therapeutic methods and become an incurable disease 1,2. MicroRNAs, which are small non-coding RNAs, post-transcriptionally regulate the expressions of their downstream target genes 3.…”

Section: Introductionmentioning

confidence: 99%

<p>MiR-155-5p accelerates the metastasis of cervical cancer cell via targeting TP53INP1</p>

Li¹,

Tao

Guo³

et al. 2019

OTT

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Background: The dysregulation of microRNAs has been implicated in the progression of different malignancies. Herein, we sought to identify the precise roles of miR-155-5p in the progression of cervical cancer. Materials and methods: The expressions of miR-155-5p in cervical carcinoma cells and clinical tissues were assessed using qRT-PCR analysis. The functions of miR-155-5p on the growth of cervical cancer cell were investigated using MTT and colony formation. The Transwell and wound closure assays were selected to explore the influence of miR-155-5p on the invasion and migration of cervical cancer cell. The effect of miR-155-5p on cervical carcinoma cell growth and metastasis in vivo was investigated using xenograft model and experimental lung metastasis model. Bioinformatics analysis and luciferase reporter assay were applied to identify that tumor protein p53-inducible nuclear protein 1 (TP53INP1) was the target of miR-155-5p. Results: MiR-155-5p was significantly upregulated in cervical cancer tissue than that in control normal tissue. Downexpression of miR-155-5p decreased the growth, migration as well as invasiveness abilities of cervical cancer cell in vitro whereas overregulation of miR-155-5p caused the opposite outcomes. In addition, the in vivo mice xenograft model suggested that downexpression of miR-155-5p restrained the progression of cervical cancer cell whereas overexpression of miR-155-5p caused opposite outcomes. Furthermore, we revealed that TP53INP1 was the target of miR-155-5p and the level of TP53INP1 was inversely associated with miR-155-5p level in cervical carcinoma. Furthermore, TP53INP1 knockdown mimicked the influence of miR-155-5p on cervical cancer proliferation, migration and invasion phenotypes. Finally, overexpression of TP53INP1 impaired the promote effect of miR-155-5p on cervical cancer cell and downregulation of TP53INP1 counteracted the suppressive impact of miR-155-5p on the aggressiveness of cervical cancer cell. Conclusion: Our study indicated that miR-155-5p regulated the development of cervical cancer cell by regulating the expression of TP53INP1.

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“…Cells encounter complex 3D extracellular matrix (ECM) in vivo . Movement of cells is affected by the ECM surface properties and physical confinement [1–3]. For example, the collective migration of epithelial and endothelial cells in capillary-like 3D structures initiates tissue development [4].…”

Section: Introductionmentioning

confidence: 99%

Cell migration on microposts with surface coating and confinement

Hui

Pang

2019

Bioscience Reports

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Understanding cell migration in a 3D microenvironment is essential as most cells encounter complex 3D extracellular matrix (ECM) in vivo. Although interactions between cells and ECM have been studied previously on 2D surfaces, cell migration studies in 3D environment are still limited. To investigate cell migration under various degrees of confinements and coating conditions, 3D platforms with micropost arrays and controlled fibronectin (FN) protein coating were developed. MC3T3-E1 cells spread and contacted the top surface of microposts if FN was coated on top. When FN was coated all over the microposts, cells were trapped between microposts with 3 μm spacing and barely moved. As the spacing between microposts increased from 3 to 5 μm, cells became elongated with limited cell movement of 0.18 μm/min, slower than the cell migration speed of 0.40 μm/min when cells moved on top. When cells were trapped in between the microposts, cell nuclei were distorted and actin filaments formed along the sidewalls of microposts. With the addition of a top cover to introduce cell confinement, the cell migration speed was 0.23 and 0.84 μm/min when the channel height was reduced from 20 to 10 μm, respectively. Cell traction force was monitored at on the top and bottom microposts with 10 μm channel height. These results show that the MC3T3-E1 cell morphology, migration speed, and movement position were affected by surface coating and physical confinement, which will provide significant insights for in vivo cell migration within a 3D ECM.

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AGER promotes proliferation and migration in cervical cancer

Cited by 16 publications

References 29 publications

Upregulation of CCT3 promotes cervical cancer progression through FN1

Upregulation of CCT3 promotes cervical cancer progression through FN1

<p>MiR-155-5p accelerates the metastasis of cervical cancer cell via targeting TP53INP1</p>

Cell migration on microposts with surface coating and confinement

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