Aggregation behaviour of a dirhamnolipid biosurfactant secreted by Pseudomonas aeruginosa in aqueous media

Sánchez, Marina; Aranda, Francisco J.; Espuny, M. J.; Marqués, Ana; Teruel, José A.; Manresa, Ángeles; Ortíz, Antonio Gómez

doi:10.1016/j.jcis.2006.11.041

Cited by 142 publications

(102 citation statements)

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“…In buffer the I 3 /I 1 ratio remains stable at 0.6 until 0.1 mM RL, after which it increases to ∼1.05 at 1 mM RL ( Figure 1B). This concentration range is in good agreement with other studies which report cmc values of rhamnolipid mixtures between 0.1 and 1 mM (Sanchez et al, 2007;Chen et al, 2010). Neither CZ nor LT affected the pyrene fluorescence pattern, indicating that the enzymes do not aid RL micelle formation below the cmc.…”

Section: Isothermal Titration Calorimetrysupporting

confidence: 92%

Microbiotechnology Based Surfactants and Their Applications

Rahman¹

2016

Frontiers Research Topics

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PublisherFrontiers in Microbiology About FrontiersFrontiers is more than just an open-access publisher of scholarly articles: it is a pioneering approach to the world of academia, radically improving the way scholarly research is managed. The grand vision of Frontiers is a world where all people have an equal opportunity to seek, share and generate knowledge. Frontiers provides immediate and permanent online open access to all its publications, but this alone is not enough to realize our grand goals. Frontiers Journal SeriesThe Frontiers Journal Series is a multi-tier and interdisciplinary set of open-access, online journals, promising a paradigm shift from the current review, selection and dissemination processes in academic publishing. All Frontiers journals are driven by researchers for researchers; therefore, they constitute a service to the scholarly community. At the same time, the Frontiers Journal Series operates on a revolutionary invention, the tiered publishing system, initially addressing specific communities of scholars, and gradually climbing up to broader public understanding, thus serving the interests of the lay society, too. Dedication to QualityEach Frontiers article is a landmark of the highest quality, thanks to genuinely collaborative interactions between authors and review editors, who include some of the world's best academicians. Research must be certified by peers before entering a stream of knowledge that may eventually reach the public -and shape society; therefore, Frontiers only applies the most rigorous and unbiased reviews. Frontiers revolutionizes research publishing by freely delivering the most outstanding research, evaluated with no bias from both the academic and social point of view. By applying the most advanced information technologies, Frontiers is catapulting scholarly publishing into a new generation. What are Frontiers Research Topics?Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: researchtopics@frontiersin.org 2 February 2016 | Microbiotechnology Based Surfactants and Their Applications Frontiers in MicrobiologyBiosurfactants are structurally diverse group of bioactive molecules produced by a variety of microorganisms. They are secondary metabolites that accumulate at interfaces, reduce surface tension and form micellar aggregates. This research topic describes few novel microbial strains with a focus on increasing our understanding of genetics, physiology, regulation of biosurfactant production and their commercial potentials. A major stumbling block in the commercialization of b...

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Section: Isothermal Titration Calorimetrysupporting

confidence: 92%

Microbiotechnology Based Surfactants and Their Applications

Rahman¹

2016

Frontiers Research Topics

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“…The CMC corresponding to at least 95% pure Rha 2 -C 14 -C 14 is between 15 and 20 mg/L. Literature values for the CMC of different Pseudomonas rhamnolipid mixtures range from 53 to 230 mg/ L [20]. The rhamnolipid Rha 2 -C 10 -C 10 of P. aeruginosa is very similar to the rhamnolipid of B. plantarii DSM 9509…”

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confidence: 95%

“…For Rha 2 -C 10 -C 10 , CMCs of 75.1 mg/L [20] and 106 mg/L [18] were found. Dubeau et al found a CMC of 225 mg/L, but they used a mixture of total rhamnolipids and HAAs (3-(3-hydroxyalkanoyloxy)alkanoic acids) of B. thailandensis E264 [13].…”

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Rhamnolipid production by Burkholderia plantarii DSM 9509^T

Hörmann

Müller

Syldatk

et al. 2010

Euro J Lipid Sci & Tech

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The Burkholderia plantarii type strain DSM 9509T was shown to produce extracellular rhamnolipids that clearly differ in structure and properties from the Pseudomonas aeruginosa rhamnolipids. The type strain has not been previously described as rhamnolipid producer. B. plantarii DSM 9509T was cultivated in a parallel bioreactor system and produced up to 45.74 mg/L of rhamnolipids when grown in glucose supplemented rich medium. The structure of the main rhamnolipid Rha 2 -C 14 -C 14 (L-rhamnopyranosyl-L-rhamnopyranosyl-b-hydroxytetradecanoyl-b-hydroxytetradecanoate) was verified by MS/MS. The purified rhamnolipid Rha 2 -C 14 -C 14 lowered the equilibrium surface tension of distilled water to 29.4 mN/m. The CMC was between 15 and 20 mg/L. In this study, growth and product formation were investigated under biotechnological aspects. Practical applications: B. plantarii DSM 9509T produces a rhamnolipid that differs in structure from P. aeruginosa rhamnolipids and exhibits excellent surfactant properties. An essential advantage in comparison to P. aeruginosa is that B. plantarii DSM 9509T is a Biosafety Level 1 microorganism and is not pathogenic in humans. A biotechnological production of the Burkholderia rhamnolipids could lead to their application in detergents, pharmaceuticals, and other industries providing new products on the biosurfactant market.

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“…These surface-active molecules can lower the surface and interfacial tension on liquid-liquid or liquid-solid phase boundaries (Pacwa-Plociniczak et al 2011;Ramani et al 2012). These compounds can be synthesized by a variety of microorganisms (bacteria, yeasts and filamentous fungi) (Sanchez et al 2007;Díaz De Rienzo et al 2016) utilizing both water-immiscible hydrocarbons i.e., plant/ animal derived oil, n-alkanes, polycyclic aromatic hydrocarbons (PAHs) and/or water-soluble compounds i.e., glucose, fructose, sucrose, xylose, galactose, mannitol, glycerol, ethanol. (Díaz De Rienzo et al 2016;Desai and Banat 1997;Mukherjee et al 2006;Sajna et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of glycolipid biosurfactant produced by a Pseudomonas otitidis strain isolated from Chirimiri coal mines, India

Singh

Tiwary

2016

Bioresour. Bioprocess.

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Background: Biosurfactants (BSs) are amphipathic, surface active molecules produced by microorganisms and can reduce the surface tension and interfacial tension. The present study emphasizes the isolation and structural characterization of the BS produced by Pseudomonas otitidis P4.Results: An efficient BS producing bacterial strain isolated from the unexplored coal mining site of Chirimiri, India was identified as P. otitidis P4 based on morphological, biochemical and 16S rRNA gene sequence analysis. The surface tension of the culture medium was reduced from 71.18 to 33.4 mN/m. The surface tension and emulsification activity of the BS remained stable over a wide range of temperature, pH and salt concentrations indicating its scope of application in bioremediation, food, cosmetics, and pharmaceutical industries. Structural attributes of BS were determined by biochemical tests, thin layer chromatography (TLC), Fourier transform infrared (FTIR) and nuclear magnetic resonance ( 1 H NMR) spectroscopy analyses, which confirmed the glycolipid nature of BS. Lipid and sugar fractions were the main constituents of the extracted BS. Thermogravimetric (TG) and Differential scanning calorimetry (DSC) analyses showed the thermostable nature of BS. As determined from TGA graph, the degradation temperature of biosurfactant was found to be 280 °C while complete weight loss was observed after 450 °C. Conclusion:The BS isolated from P. otitidis P4 was identified as glycolipid and showed high emulsification activity and stability in a wide range of temperature, pH and salinity which makes it suitable for various industrial and environmental applications.

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Aggregation behaviour of a dirhamnolipid biosurfactant secreted by Pseudomonas aeruginosa in aqueous media

Cited by 142 publications

References 35 publications

Microbiotechnology Based Surfactants and Their Applications

Microbiotechnology Based Surfactants and Their Applications

Rhamnolipid production by Burkholderia plantarii DSM 9509^T

Isolation and characterization of glycolipid biosurfactant produced by a Pseudomonas otitidis strain isolated from Chirimiri coal mines, India

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Aggregation behaviour of a dirhamnolipid biosurfactant secreted by Pseudomonas aeruginosa in aqueous media

Cited by 142 publications

References 35 publications

Microbiotechnology Based Surfactants and Their Applications

Microbiotechnology Based Surfactants and Their Applications

Rhamnolipid production by Burkholderia plantarii DSM 9509T

Isolation and characterization of glycolipid biosurfactant produced by a Pseudomonas otitidis strain isolated from Chirimiri coal mines, India

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Rhamnolipid production by Burkholderia plantarii DSM 9509^T