Aggregation‐Induced Emission: Lighting up Cells, Revealing Life!

Wang, Yifeng; Zhang, Tingbin; Liang, Xing‐Jie

doi:10.1002/smll.201601468

Cited by 121 publications

(64 citation statements)

References 148 publications

(170 reference statements)

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“…Compared with commercial biosensors, AIEgens are popular due to their photobleaching resistance, large Stokes shift and excellent brightness. Through exquisite design, many AIE‐active materials have been developed in biological applications …”

Section: Applications Of Aie Probes In the Field Of Biological Sciencementioning

confidence: 99%

“…Although the progress concerning AIE-based materials has been reviewed recently, most of the reviews summarized material fabrication, [27][28][29] molecular structural design, 30,31 analyte type, 32,33 and biosensing. 34,35 This review focuses on the recent progress in AIE-active materials applied in the field of biological science and tries to provide the latest examples. In addition, features of this review are as follows: (a) we try to summarize the mechanisms including but not limited to RIM.…”

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confidence: 99%

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A new approach to developing diagnostics and therapeutics: Aggregation‐induced emission‐based fluorescence turn‐on

Guo

Song

et al. 2019

Medicinal Research Reviews

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Fluorescence imaging is a promising visualization tool and possesses the advantages of in situ response and facile operation; thus, it is widely exploited for bioassays. However, traditional fluorophores suffer from concentration limits because they are always quenched when they aggregate, which impedes applications, especially for trace analysis and real‐time monitoring. Recently, novel molecules with aggregation‐induced emission (AIE) characteristics were developed to solve the problems encountered when using traditional organic dyes, because these new molecules exhibit weak or even no fluorescence when they are in free movement states but emit intensely upon the restriction of intramolecular motions. Inspired by the excellent performances of AIE molecules, a substantial number of AIE‐based probes have been designed, synthesized, and applied to various fields to fulfill diverse detection tasks. According to numerous experiments, AIE probes are more practical than traditional fluorescent probes, especially when used in bioassays. To bridge bioimaging and materials engineering, this review provides a comprehensive understanding of the development of AIE bioprobes. It begins with a summary of mechanisms of the AIE phenomenon. Then, the strategies to realize accurate detection using AIE probes are discussed. In addition, typical examples of AIE‐active materials applied in diagnosis, treatment, and nanocarrier tracking are presented. In addition, some challenges are put forward to inspire more ideas in the promising field of AIE‐active materials.

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Section: Applications Of Aie Probes In the Field Of Biological Sciencementioning

confidence: 99%

mentioning

confidence: 99%

A new approach to developing diagnostics and therapeutics: Aggregation‐induced emission‐based fluorescence turn‐on

Guo

Song

et al. 2019

Medicinal Research Reviews

View full text Add to dashboard Cite

show abstract

“…In recent decades, AIEgens have been proved to possess high photostability, large Stokes shi, excellent capability of longterm tracking and good biocompatibility. [55][56][57][58][59][60][61][62][63][64][65][66][67] Although great attention has been paid to tissue regeneration and lysosome study, 4,5,27,28,[68][69][70] monitoring lysosomal pH during tissue regeneration in vivo has not been investigated yet.…”

Section: Introductionmentioning

confidence: 99%

In vivomonitoring of tissue regeneration using a ratiometric lysosomal AIE probe

et al. 2020

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“…On the other hand, hydrophobic AIEgens are excellent candidates to synthesize fluorescent NPs with high loading doses to achieve desired brightness and excellent photobleaching resistance . Along with the rapid development of AIEgens in the past decades, several exciting reviews have been published . Nevertheless, most of them focused on the discovery, mechanism, property, and molecular library of AIEgens, as well as their applications in different research areas.…”

Section: Introductionmentioning

confidence: 99%

Unity Makes Strength: How Aggregation‐Induced Emission Luminogens Advance the Biomedical Field

et al. 2018

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research fields to provide new opportunities for precise and personalized medical treatment of patients. [16][17][18][19][20][21][22][23][24][25][26][27] An ideal fluorescent agent should have low toxicity, high brightness, excellent stability, good selectivity, and versatile functionality. To date, a vast variety of synthetic fluorescent materials, including organic dyes, [28][29][30] fluorescent proteins, [31,32] inorganic quantum dots (QDs), [33,34] conjugated polymer nanoparticles (NPs), [35][36][37][38][39][40] metallic nanoclusters, [41] as well as upconversion NPs, [42][43][44] have been explored and demonstrated for biological applications.Among the above-mentioned fluorescent materials, synthetic organic dyes and dye-loaded fluorescent NPs have attracted special attention due to the good biocompatibility and easy availability. However, a considerable number of organic dyes are hydrophobic, which often form aggregation through π-π stacking in aqueous solutions and buffers even though different strategies have been applied to improve their water solubility. The serious π-π stacking interactions among dye molecules facilitate nonradiative pathways to relax the exciton energy, leading to partially or completely quenched fluorescence. This phenomenon is well known as aggregation-caused quenching (ACQ) effect, [45] which is the main obstacle of construction of highly emissive fluorescence bioprobes in complicated biological microenvironments. Although great efforts have been made from various aspects to overcome the notorious undesired ACQ effect, [46] it remains a major challenge for the significant improvement of traditional fluorescent bioprobes in biomedicine and life sciences.In 2001, Tang and co-workers discovered a novel fluorescent phenomenon that is opposite to the ACQ effect: the organic Figure 4. Confocal images of Hela cancer cells after incubation with A-D) A6-A9 (5 × 10 −6 m) for 15 min, respectively. Adapted with permission. [104]

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Aggregation‐Induced Emission: Lighting up Cells, Revealing Life!

Cited by 121 publications

References 148 publications

A new approach to developing diagnostics and therapeutics: Aggregation‐induced emission‐based fluorescence turn‐on

A new approach to developing diagnostics and therapeutics: Aggregation‐induced emission‐based fluorescence turn‐on

In vivomonitoring of tissue regeneration using a ratiometric lysosomal AIE probe

Unity Makes Strength: How Aggregation‐Induced Emission Luminogens Advance the Biomedical Field

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