Aggregative properties of different cell types of the fresh-water spongeEphydatia fluviatilis isolated on ficoll gradients

Sutter, Danielle De; Vyver, Gisèle Van De

doi:10.1007/bf00848439

Cited by 49 publications

(32 citation statements)

References 9 publications

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“…A FicollVrografin density gradient protocol was developed by integrating several published protocols (Zhang et al 2003b;De Sutter and Van de Vyver 1977;Uriz et al 1996;Yin and Humphreys 1996) to purify the archaeocytes. Solutions of 3% Ficoll (F) and 38% Vrografin (V) were prepared in sterile CMFSW at a density of 1.0276 g/ml and 1.1486 g/ml, respectively.…”

Section: Archaeocyte Purificationmentioning

confidence: 99%

“…The isolation and purification of specific cell types for the development of cell culture in vitro is therefore difficult. Ficoll or Percoll density gradient centrifugation has been used to fractionate various sponge cell types in order to investigate their aggregative properties (De Sutter and Van de Vyver 1977). These methods have also been employed in the localization of bioactive metabolites in sponge cells (Uriz et al 1996;Flowers et al 1998;Salomon et al 2001).…”

Section: Introductionmentioning

confidence: 98%

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Purification and in vitro cultivation of archaeocytes (stem cells) of the marine sponge Hymeniacidon perleve (Demospongiae)

Sun

Song

et al. 2006

Cell Tissue Res

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Marine sponges (Porifera) are the best source of marine bioactive metabolites for drug discovery and development, although the sustainable production of most sponge-derived metabolites remains a difficult task. In vitro cultivation of sponge cells in bioreactors has been proposed as a promising technology. However, no continuous cell line has as yet been developed. Archaeocytes are considered to be toti/multipotent stem cells in sponges and, when purified, may allow the development of continuous sponge cell lines. As a prerequisite, we have developed a novel four-step protocol for the purification of archaeocytes from a marine sponge, Hymeniacidon perleve: (1) differential centrifugation to separate large sponge cells including archaeocytes; (2) selective agglomeration in low-Ca(2+)/Mg(2+) artificial seawater in which living archaeocytes form small loose aggregates with some pinacocytes and collencytes; (3) differential adherence to remove anchorage-dependent pinacocytes, collencytes and other mesohyl cells; (4) Ficoll-Vrografin density gradient centrifugation to purify archaeocytes. The final purity of archaeocytes is greater than 80%. The proliferation potential of the archaeocytes has been demonstrated by high levels of BrdU incorporation, PCNA expression and telomerase activity. In 4-day primary cultures, the purified archaeocytes show a 2.5-fold increase in total cell number. This study opens an important avenue towards developing sponge cell cultures for the commercial exploitation of sponge-derived drugs.

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Section: Archaeocyte Purificationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Purification and in vitro cultivation of archaeocytes (stem cells) of the marine sponge Hymeniacidon perleve (Demospongiae)

Sun

Song

et al. 2006

Cell Tissue Res

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“…The cell suspensions obtained can be used to produce small sponge aggregates that may form the starting point for an in vitro culture. Artificial sponge cell dissociation, combined with sponge cell separation techniques using density gradients (De Sutter and Van de Vyver, 1977;De Sutter and Tulp, 1981), is also used to produce primary cell lines for sponge cell culture (see Cell Cultures, below).…”

Section: Short-term Cultivationmentioning

confidence: 99%

Cultivation of Marine Sponges

1999

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There is increasing interest in biotechnological production of marine sponge biomass owing to the discovery of many commercially important secondary metabolites in this group of animals. In this article, different approaches to producing sponge biomass are reviewed, and several factors that possibly influence culture success are evaluated. In situ sponge aquacultures, based on old methods for producing commercial bath sponges, are still the easiest and least expensive way to obtain sponge biomass in bulk. However, success of cultivation with this method strongly depends on the unpredictable and often suboptimal natural environment. Hence, a better-defined production system would be desirable. Some progress has been made with culturing sponges in semicontrolled systems, but these still use unfiltered natural seawater. Cultivation of sponges under completely controlled conditions has remained a problem. When designing an in vitro cultivation method, it is important to determine both qualitatively and quantitatively the nutritional demands of the species that is to be cultured. An adequate supply of food seems to be the key to successful sponge culture. Recently, some progress has been made with sponge cell cultures. The advantage of cell cultures is that they are completely controlled and can easily be manipulated for optimal production of the target metabolites. However, this technique is still in its infancy: a continuous cell line has yet to be established. Axenic cultures of sponge aggregates (primmorphs) may provide an alternative to cell culture. Some sponge metabolites are, in fact, produced by endosymbiotic bacteria or algae that live in the sponge tissue. Only a few of these endosymbionts have been cultivated so far. The biotechnology for the production of sponge metabolites needs further development. Research efforts should be continued to enable commercial exploitation of this valuable natural resource in the near future.

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“…In Ophlitaspongia seriata, only the cells dissociated from the larvae originate small functional sponges, whereas those from adults are unable to do this (Borojevic & Lévi, 1964). The function and the role performed by different cell types in the ensuing aggregates is better understood by using the technique of cell type isolation, allowing homogeneous cell categories to be used, and by testing the morphogenetic properties of the aggregates in relation with the original cell source (John et al, 1971;De Sutter & Buscema, 1977;De Sutter & Van de Vyver, 1977). In Ephydatia fluviatilis, this technique demonstrated that archaeocytes are able to reconstitute functional sponges without any other cell type (Buscema et al, 1980).…”

Section: Cell Responses During Aggregation In Vitromentioning

confidence: 99%

Self/non‐self recognition in sponges

Gaino

Bavestrello

Magnino

1999

Italian Journal of Zoology

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A variety of procedures have been utilized to study the sponge ability to discriminate self-from non-self. In adult sponges, the histocompatibility reaction has traditionally been tested by transplantation techniques or parabiosis. These consist of pushing into contact different species (xenograft) or conspecific individuals (allograft) with their outer pinacodermal layers intact. A fast reaction can be observed when fragments of the inner part of the sponge are tied together. Lack of compatibility generates signals suppressing or activating both exopinacocytes bounding the contact surface and discrete mesohyl cell types (archaeocytes, collencytes, gray and spherulous cells). These cells are involved to different extents according to the species (active migration along the contact surface, cytotoxicity, phagocytosis, synthesis of a collagen barrier). In sponge cell aggregates, species-specific sorting-out expresses the recognition reaction of the mixed cells. Glycoconjugates are key molecules for self/non-self cell discrimination. In the model derived from Geodia cydonium cells, galectin molecules in the presence of Ca 2+ form a bridge between the intercellular multiprotein complex aggregation factor (AF) and the cell surface-associated aggregation receptor (AR). The AF is a soluble and diffusible macromolecule that may play an important role also in self recognition mechanisms occurring in sponges in toto. A new paradigm has recently been suggested for cell recognition: interactions between the most peripheral cell surface specific proteoglycans (glyconectins). These molecules have been purified from three marine sponge species (Microciona prolifera, Halichondria panicea, and Cliona celata). This mechanism of self recognition could represent a remarkable event for the appearance of multicellularity.

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Aggregative properties of different cell types of the fresh-water spongeEphydatia fluviatilis isolated on ficoll gradients

Cited by 49 publications

References 9 publications

Purification and in vitro cultivation of archaeocytes (stem cells) of the marine sponge Hymeniacidon perleve (Demospongiae)

Purification and in vitro cultivation of archaeocytes (stem cells) of the marine sponge Hymeniacidon perleve (Demospongiae)

Cultivation of Marine Sponges

Self/non‐self recognition in sponges

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