Aging and the Biological Clock

Judge, Michael T.; Griffith, James W.; Arnold, Jonathan

doi:10.1007/978-3-319-64543-8_10

Cited by 7 publications

(9 citation statements)

References 121 publications

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“…How the cells socialize [88] involves the interaction of cells that can be manipulated in a microfluidics device. How an organism ages can be examined directly as single cells age in different stressful environments [5].…”

Section: Discussionmentioning

confidence: 99%

“…6) at work including a positive role for noise in synchronization of individual cells [20], [30]. The synchronization of single cell oscillators in tissues is hypothesized to have roles in disease [68], [69] and aging [5].…”

Section: E Single Cells Display Temperature Compensationmentioning

confidence: 99%

“…This synchronization occurs at other scales within organisms as well. Most organisms possess biological clocks [5], the ability to tell time so that an organism, eats, sleeps, or reproduces at the appropriate time, as examples. These biological clocks are observed at the level of 10 7 cells or more [6], where there is tight synchronization of the circadian rhythm between cells.…”

Section: Introductionmentioning

confidence: 99%

“…Most studies have confined themselves to demonstrating circadian oscillations in single cells [8], [12] and have not examined the other two basic properties of a biological clock (with the exception of light entrainment in a synthetic system [13]), light entrainment and temperature compensation [5]. Temperature compensation here represents the ability to keep time when temperature fluctuates within physiological limits.…”

Section: Introductionmentioning

confidence: 99%

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Single Cells of Neurospora Crassa Show Circadian Oscillations, Light Entrainment, Temperature Compensation, and Phase Synchronization

et al. 2019

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Using a microfluidics device, fluorescence of a recorder (mCherry or mVenus) gene driven by a clock-controlled gene-2 promoter (ccg-2p) was measured simultaneously on over 1000 single cells of Neurospora crassa every half hour for 10 days under each of varied light and temperature conditions. Single cells were able to entrain to light over a wide range of day lengths, including 6, 12, or 36 h days. In addition, the period of oscillations in fluorescence remained remarkably stable over a physiological range of temperatures from 20 o C to 30 o C (Q 10 = 1.00-1.07). These results provide evidence of an autonomous clock in most single cells of N. crassa. While most cells had clocks, there was substantial variation between clocks as measured by their phase, raising the question of how such cellular clocks in single cells phase-synchronize to achieve circadian behavior in eukaryotic systems at the macroscopic level of 10 7 cells, where most measurements on the clock are performed. Single cells were placed out of phase by allowing one population to receive 6 or 12 h more light before lights out (D/D). The average phase difference was reduced in the mixed population relative to two unmixed control populations.

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Section: Discussionmentioning

confidence: 99%

Section: E Single Cells Display Temperature Compensationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Single Cells of Neurospora Crassa Show Circadian Oscillations, Light Entrainment, Temperature Compensation, and Phase Synchronization

et al. 2019

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“…Other models have demonstrated a possible role played by reactive oxygen species in both aging and clock function (Gyongyosi and Kaldi 2013) and more recently in the Ras-Erk-ETS signaling pathway (Slack et al, 2015). A variety of metabolic linkages may exist between the clock and aging (Judge, Griffith, & Arnold, 2017).…”

Section: Introductionmentioning

confidence: 99%

lac‐1 and lag‐1 with ras‐1 affect aging and the biological clock in Neurospora crassa

Brunson

Griffith

Bowles

et al. 2016

Ecology and Evolution

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Using an automated cell counting technique developed previously (Case et al., Ecology and Evolution 2014; 4: 3494), we explore the lifespan effects of lac‐1, a ceramide synthase gene paralogous to lag‐1 in Neurospora crassa in conjunction with the band bd (ras‐1) gene. We find that the replicative lifespan of a lac‐1 KO bd double mutants is short, about one race tube cycle, and this double mutant lacks a strong ~21‐hr clock cycle as shown by race tube and fluorometer analysis of fluorescent strains including lac‐1 KO. This short replicative lifespan phenotype is contrasted with a very long estimated chronological lifespan for lac‐1 KO bd double mutants from 247 to 462 days based on our regression analyses on log viability, and for the single mutant lac‐1 KO, 161 days. Both of these estimated lifespans are much higher than that of previously studied WT and bd single mutant strains. In a lac‐1 rescue and induction experiment, the expression of lac‐1 + as driven by a quinic acid‐dependent promoter actually decreases the median chronological lifespan of cells down to only 7 days, much lower than the 34‐day median lifespan found in control bd conidia also grown on quinic acid media, which we interpret as an effect of balancing selection acting on ceramide levels based on previous findings from the literature. Prior work has shown phytoceramides can act as a signal for apoptosis in stressed N. crassa cells. To test this hypothesis of balancing selection on phytoceramide levels, we examine the viability of WT, lag‐1 KO bd, and lac‐1 KO bd strains following the dual stresses of heat and glycolysis inhibition, along with phytoceramide treatments of different dosages. We find that the phytoceramide dosage–response curve is altered in the lag‐1 KO bd mutant, but not in the lac‐1 KO bd mutant. We conclude that phytoceramide production is responsible for the previously reported longevity effects in the lag‐1 KO bd mutant, but a different ceramide may be responsible for the longevity effect observed in the lac‐1 KO bd mutant.

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Using evolutionary genomics, transcriptomics, and systems biology to reveal gene networks underlying fungal development

Wang

Gudibanda

Ugwuowo

et al. 2018

Fungal Biology Reviews

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Aging and the Biological Clock

Cited by 7 publications

References 121 publications

Single Cells of Neurospora Crassa Show Circadian Oscillations, Light Entrainment, Temperature Compensation, and Phase Synchronization

Single Cells of Neurospora Crassa Show Circadian Oscillations, Light Entrainment, Temperature Compensation, and Phase Synchronization

lac‐1 and lag‐1 with ras‐1 affect aging and the biological clock in Neurospora crassa

Using evolutionary genomics, transcriptomics, and systems biology to reveal gene networks underlying fungal development

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