Aging erythrocyte membranes as biomimetic nanometer carriers of liver-targeting chromium poisoning treatment

Abstract

Chromium poisoning has become one of the most common heavy metal poisoning occupational diseases with high morbidity and mortality. However, most antidotes detoxify the whole body and are highly toxic. To achieve hepato-targeted chromium poisoning detoxification, a novel hepato-targeted strategy was developed using aging erythrocyte membranes (AEMs) as biomimetic material coated with a dimercaptosuccinic acid (DMSA) nanostructured lipid carrier to construct a biomimetic nanodrug delivery system. The particle size, potential, drug loading, encapsulation rate, in vitro release, and stability of the nanoparticles (NPs) were characterized. Confocal microscopy and flow cytometry showed that the prepared NPs could be phagocytized by RAW264.7 macrophage cells. The efficacy of AEM-DMSA-NPs for targeted liver detoxification was evaluated by in vitro MTT analysis and an in vivo model of chromium poisoning. The results showed that the NPs could safely and efficiently achieve targeted liver chromium poisoning detoxification. All the results indicated that the biomimetic nanodrug delivery system mediated by aging erythrocyte membranes and containing DMSA nanoparticles could be used as a novel therapeutic drug delivery system potentially targeting liver detoxification.

Red blood cell-hitchhiking mediated pulmonary delivery of ivermectin: Effects of nanoparticle properties

“…NPs adsorbed to RBCs could avoid MPS uptake, which prolonged their circulation time. A macrophage uptake experiment was used to evaluate the anti-phagocytosis abilities of RBC-PNPs and RBC-CSPNPs ( Yao et al, 2021 ). The fluorescence intensity of Cy5.5-PNPs and Cy5.5-CSPNPs groups were significantly higher than those of the RBC-Cy5.5-PNPs and RBC-Cy5.5-CSPNPs groups, implying rapid clearance of PNPs and CSPNPs in vivo ( Fig.…”

Cepharanthine loaded nanoparticles coated with macrophage membranes for lung inflammation therapy

“…To verify whether MM-NLCs inherit the immunological characteristics of macrophages, the in vitro antiphagocytosis assay was first conducted. RAW264.7 cells were utilized as a cell model to reckon the stealth power of the biomimetic nanosystem (Xuan et al., 2016 ; Yao et al., 2021 ). The captured images showed that a less fluorescence level in macrophages treated with MM-NLCs than in the bare NLCs group ( Figure 4(A) ).…”

The Effect of Fullerenol C60(OH)36 on the Antioxidant Defense System in Erythrocytes

“…In the present study, we also examined the microviscosity of the interior of the erythrocyte in the presence and absence of C 60 (OH) 36 . Aging of erythrocytes is accompanied by an increase in the cell microviscosity of cells [46] because of a decrease in intracellular electrolyte concentration, a lower water content, and a reduction in cell diameter, volume, and surface area [32,47]. The cytoplasmic microviscosity of the interior of erythrocytes is determined mainly by the concentration of hemoglobin, but also depends on the water content of the cell [48].…”

“…Mature human erythrocytes constitute a convenient research model to study oxidative damage during prolonged incubation, which triggers ROS-mediated damage [ 30 , 31 , 32 , 33 , 34 ]. The source of ROS in erythrocytes is hemoglobin, which undergoes autoxidation to methemoglobin producing O 2 •− [ 30 ].…”