AgNOR mark epithelial foci in malignant transformation in hamster cheek pouch carcinogenesis

Schwint, Amanda E.; Folco, A.; Morales, Azorides R.; Cabrini, R. L.; Itoiz, M. E.

doi:10.1111/j.1600-0714.1996.tb01218.x

Cited by 17 publications

(18 citation statements)

References 31 publications

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“…Our results reaffirm the finding of previous studies, 32,33 that is, an increase in AgNOR counts in dysplastic leukoplakia, compared with non‐dysplastic leukoplakia and NOM. Thus, the AgNOR might be quantitative, discriminative, and easy to monitor routinely in detecting incipient cellular alterations 34 . In our study, the mean AgNOR count for leukoplakia with dysplasia was 2.73.…”

Section: Discussionmentioning

confidence: 52%

Actual proliferating index in oral squamous cell carcinoma and leukoplakia

Chandak¹,

Gadbail

Chaudhary

et al. 2011

J of Invest & Clin Dent

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Section: Discussionmentioning

confidence: 52%

Actual proliferating index in oral squamous cell carcinoma and leukoplakia

Chandak¹,

Gadbail

Chaudhary

et al. 2011

J of Invest & Clin Dent

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“…Positive correlation has been described between the number of AgNORs and cell proliferative activity. 17 The group of less recognized factors includes metallothioneins (MT), the group of low molecular weight proteins (6-7 kDa), containing 61 to 62 amino acids each. These proteins are characterized by high content of cystein residues (23 to 33%) and low content of aromatic and hydrophobic amino acids.…”

Section: Introductionmentioning

confidence: 99%

Steroid receptor status, proliferation and metallothionein expression in primary invasive ductal breast cancers

Surowiak¹,

Paluchowski

Wysocka³

et al. 2004

Pathol. Oncol. Res.

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The most important immunocytochemical prognostic and predictive factors in cases of breast cancer include estrogen receptor alpha (ER) and progesterone receptor (PgR). The present study aimed at examining the relationship between the manifestation intensity of proliferation markers (Ki-67 and nucleolar organizer regions--AgNORs) on one hand, and expression of ER and PgR on the other in a uniform group of invasive ductal breast cancers of G2 grade. Moreover, the study aimed at examining the relationship between the above mentioned markers and expression of metallothionein (MT). The studies were performed on samples of invasive ductal breast cancers of G2 grade, originating from 60 females. In paraffin sections originating from the studied cases immunocytochemical reactions were performed using monoclonal antibodies to ER, PgR, Ki-67 and MT, and silver staining was conducted to localize AgNORs. The obtained results were subjected to statistical analysis using Statistica software. Results indicate that manifestation of AgNORs does not correlate with any of the studied antigens (ER, PgR, Ki-67, MT) (p>0.05). Moreover, no relationship could be demonstrated between the intensity of MT expression and proliferation markers or steroid receptor status (p>0.05). A negative correlation was shown between the expression of ER and Ki-67 (p=0.0009). The most intense proliferative activity was demonstrated in cases of breast cancer showing PgR expression but no ER expression (p=0.015), while the lowest proliferative activity was detected in breast cancers with expression of both ER and PgR (p<0.05).

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“…As described above, within certain constraints, this area can be considered a model of early cancerization. Finally, we examined the value of this technique to mark epithelial foci in malignant transformation in the hamster cheek pouch model .…”

Section: Histochemical Markers Of Field Cancerization Demonstrable Inmentioning

confidence: 99%

The hamster cheek pouch model for field cancerization studies

Monti-Hughes¹,

Aromando²,

Pérez³

et al. 2014

Periodontology 2000

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External carcinogens, such as tobacco and alcohol, induce molecular changes in large areas of oral mucosa, which increase the risk of malignant transformation. This condition, known as 'field cancerization', can be detected in biopsy specimens using histochemical techniques, even before histological alterations are identified. The efficacy of these histochemical techniques as biomarkers of early cancerization must be demonstrated in appropriate models. The hamster cheek pouch oral cancer model, universally employed in biological studies and in studies for the prevention and treatment of oral cancer, is also an excellent model of field cancerization. The carcinogen is applied in solution to the surface of the mucosa and induces alterations that recapitulate the stages of cancerization in human oral mucosa. We have demonstrated that the following can be used for the early detection of cancerized tissue: silver staining of nucleolar organizer regions; the Feulgen reaction to stain DNA followed by ploidy analysis; immunohistochemical analysis of fibroblast growth factor-2, immunohistochemical labeling of proliferating cells to demonstrate an increase of epithelial cell proliferation in the absence of inflammation; and changes in markers of angiogenesis (i.e. those indicating vascular endothelial growth factor activity, endothelial cell proliferation and vascular density). The hamster cheek pouch model of oral cancer was also proposed and validated by our group for boron neutron capture therapy studies for the treatment of oral cancer. Clinical trials of this novel treatment modality have been performed and are underway for certain tumor types and localizations. Having demonstrated the efficacy of boron neutron capture therapy to control tumors in the hamster cheek pouch oral cancer model, we adapted the model for the long-term study of field cancerized tissue. We demonstrated the inhibitory effect of boron neutron capture therapy on tumor development in field cancerized tissue with acceptable levels of mucositis, a dose-limiting side-effect.

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AgNOR mark epithelial foci in malignant transformation in hamster cheek pouch carcinogenesis

Cited by 17 publications

References 31 publications

Actual proliferating index in oral squamous cell carcinoma and leukoplakia

Actual proliferating index in oral squamous cell carcinoma and leukoplakia

Steroid receptor status, proliferation and metallothionein expression in primary invasive ductal breast cancers

The hamster cheek pouch model for field cancerization studies

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