Agrobacterium-mediated PsCBL and PsCIPK gene transformation to enhance salt tolerance in indica rice (Oryza sativa)

Ectopic expression of Vigna radiata's vacuolar Na+/H+ antiporter gene (VrNHX1) in indica rice (Oryza sativa L.)

“…The appropriate acetosyringone concentration, which ranges between 100 and 400 µM depending on the genotype, is another critical component for successful transformation. Previous research has shown that adding 150 µM acetosyringone boosts transformation efficiency in rice and other plants [ 53 , 54 ] and we found transformants in this investigation by adding this quantity of acetosyringone. With the advancement of plant biotechnology, an Agrobacterium- mediated genetic transformation is now a popular approach for generating stress and disease-resistant plants [ 55 , 56 ].…”

“…With the advancement of plant biotechnology, an Agrobacterium- mediated genetic transformation is now a popular approach for generating stress and disease-resistant plants [ 55 , 56 ]. Salinity stress is a severe limitation on rice production, particularly in coastal areas, and it is worsening in climate-vulnerable nations such as Bangladesh, where rice is the primary source of food [54] . To restore rice farming to these saline-affected areas, salt-tolerant rice varieties must be developed.…”

Trends in the Use of Tissue Culture, Applications and Future Aspects

“…46 Genetically engineered cells are developed form transgenic whole plants also require tissue culture process; apart from this formation of somatic haploid embryos need the help of these techniques thus it has been, prominent in academic and applied plant science. 42,47…”