Agrococcus terreus sp. nov. and Micrococcus terreus sp. nov., isolated from forest soil

Abstract

Two bacterial strains, DNG5T and V3M1T, isolated from forest soil of the Changbai mountains in China, were characterized using a polyphasic approach. Analysis of their 16S rRNA gene sequences indicated that strains DNG5T and V3M1T were phylogenetically related to members of the genus Agrococcus (96.0–98.4 % similarity) and Micrococcus (96.7–98.0 % similarity), respectively, within the order Actinomycetales. Strains DNG5T and V3M1T were Gram-stain-positive and strictly aerobic and formed yellow colonies on LB agar. Cells of strain DNG5T were short, non-motile rods, 0.4–0.5×0.8–1.0 μm. Strain DNG5T contained MK-10 and MK-11 as the major respiratory quinones and anteiso-C15 : 0 (49.2 %) and iso-C16 : 0 (22.4 %) as the major fatty acids. The diamino acid in the peptidoglycan of strain DNG5T was 2,4-diaminobutyric acid and the murein was of the acetyl type. Cells of strain V3M1T were cocci, 0.6–0.7 μm in diameter. The cell-wall peptidoglycan of strain V3M1T contained the amino acids lysine, glutamic acid, alanine and glycine. Strain V3M1T contained MK-7, MK-7(H2), MK-8 and MK-8(H2) as respiratory quinones and anteiso-C15 : 0 (78.2 %) and iso-C15 : 0 (13.1 %) as the major cellular fatty acids. The DNA G+C contents of strains DNG5T and V3M1T were 75.9 and 67.2 mol%, respectively. The DNA–DNA relatedness of strain DNG5T to Agrococcus jejuensis DSM 22002T, A. jenensis JCM 9950T, A. baldri JCM 12132T and A. citreus JCM 12398T was 58.3, 43.9, 36.1 and 54.1 %, respectively. The DNA–DNA relatedness of strain V3M1T to Micrococcus luteus CGMCC 1.2299T, M. antarcticus CGMCC 1.2373T and M. lylae CGMCC 1.2300T was 57.5, 45.4 and 39.0 %, respectively. Combining phenotypic and genotypic traits, strain DNG5T represents a novel species of the genus Agrococcus, for which the name Agrococcus terreus sp. nov. is proposed, with DNG5T (=CGMCC 1.6960T =NBRC 104260T) as the type strain. Strain V3M1T represents a novel species of the genus Micrococcus, for which the name Micrococcus terreus sp. nov. is proposed, with V3M1T (=CGMCC 1.7054T =NBRC 104258T) as the type strain.

Bacterial isolates fromPalomena prasina(Hemiptera: Pentatomidae) include potential microbial control agents

“…Therefore, we classified this isolate as A. jejuensis. Members of the genus Agrococcus have been isolated from various environments including soil, cheese surfaces, potato plants and dried seaweed as well as from the air (Zhang, Liu, & Liu, 2010). In this study, an Agrococcus species was isolated for the first time from an insect.…”

Response of cellular fatty acids to environmental stresses in endophytic Micrococcus spp.

“…strain HBL-15 revealed that the bacterium did not show any response to increasing salt concentration, and the cellular fatty acid composition was almost similar at all three different concentrations of salt (Table 4). It indicated that, due to structural differences in the cellular envelope of Gram-positive and Gram-negative bacteria (Zhang et al 2010); Micrococcus and Halomonas showed different responses for salt stress. As discussed earlier, iso and anteiso branched-chain fatty acids are characteristic of the Grampositive cell wall, but this kind of fatty acid is lacking in the cell wall of Gram-negative bacteria.…”

Effect of Growth Media and Incubation Time on the Culturability of Soil Bacteria

“…By increasing the incubation time as suggested by [6], we should be able to isolate some members of groups that to date have not been grown in pure culture. According to [14] and [13] the use of serial dilution, liquid culture techniques has proved to be effective for isolating numerically significant members of the community in cultivated and non-cultivated soil samples. However, when this techniques was used to cultivate bacteria from the Nutrient agar, Plate count agar and Soil extract agar, the mean viable count obtained were 45.5%, 36.5% and 18.0% for sample A (cultivated sandy loamy soil) and 51.9%.…”