AHL communication is a widespread phenomenon in biomining bacteria and seems to be involved in mineral-adhesion efficiency

Ruíz, Lina; Valenzuela, Susana V.; Castro, Matías; González, Alex; Frezza, Marine; Soulère, Laurent; Rohwerder, Thore; Queneau, Yves; Doutheau, Alain; Sand, Wolfgang; Jerez, Carlos A.; Guiliani, Nicolás

doi:10.1016/j.hydromet.2008.05.028

Cited by 61 publications

(44 citation statements)

References 25 publications

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“…This included a luxI family member, mbaI (T451DRAFT_0796), as well as a luxR family member, mbaR (T451DRAFT_0820). MbaI shares 68% amino acid sequence identity with the AHL synthase from the acidophilic bacterium Acidithiobacillus thiooxidans, strains of which have been reported to produce primarily N-3-oxo-octanoyl-Lhomoserine lactone (3-oxo-C 8 -HSL) (16).…”

Section: Resultsmentioning

confidence: 99%

Quorum Sensing in a Methane-Oxidizing Bacterium

Puri

Schaefer

et al. 2017

J Bacteriol

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Aerobic methanotrophic bacteria use methane as their sole source of carbon and energy and serve as a major sink for the potent greenhouse gas methane in freshwater ecosystems. Dissecting the molecular details of how these organisms interact in the environment may increase our understanding of how they perform this important ecological role. Many bacterial species use quorum sensing (QS) systems to regulate gene expression in a cell density-dependent manner. We have identified a QS system in the genome of Methylobacter tundripaludum, a dominant methane oxidizer in methane enrichments of sediment from Lake Washington (Seattle, WA). We determined that M. tundripaludum produces primarily N-3-hydroxydecanoyl-L-homoserine lactone (3-OH-C 10 -HSL) and that its production is governed by a positive feedback loop. We then further characterized this system by determining which genes are regulated by QS in this methane oxidizer using transcriptome sequencing (RNA-seq) and discovered that this system regulates the expression of a putative nonribosomal peptide synthetase biosynthetic gene cluster. Finally, we detected an extracellular factor that is produced by M. tundripaludum in a QS-dependent manner. These results identify and characterize a mode of cellular communication in an aerobic methane-oxidizing bacterium.IMPORTANCE Aerobic methanotrophs are critical for sequestering carbon from the potent greenhouse gas methane in the environment, yet the mechanistic details of chemical interactions in methane-oxidizing bacterial communities are not well understood. Understanding these interactions is important in order to maintain, and potentially optimize, the functional potential of the bacteria that perform this vital ecosystem function. In this work, we identify a quorum sensing system in the aerobic methanotroph Methylobacter tundripaludum and use both chemical and genetic methods to characterize this system at the molecular level.KEYWORDS methane, methanotroph, quorum sensing, sociomicrobiology, acyl-homoserine lactone, biosynthetic gene cluster A erobic methane-oxidizing bacteria (methanotrophs) are an important component of the carbon cycle that serve to sequester carbon from the potent greenhouse gas methane after it is produced by anaerobic microbial ecosystems (1, 2). Methanotrophs provide a carbon and energy source for communities of organisms that cannot oxidize methane themselves, thereby serving as key supporting species in the environment (3, 4). The methanotroph Methylobacter tundripaludum is a member of the Gammaproteobacteria (type I methanotroph), and it has been repeatedly identified as a dominant member of methane enrichment and stable isotope probing experiments of sediment from Lake Washington, Seattle, WA (3, 5-7). Methylobacter spp. have also been isolated from other geographically distinct regions, including the arctic (8), estuaries (9), and temperate wetlands (10), highlighting the diverse environments inhabited by this genus. We, therefore, use M. tundripaludum as part of a model system for ...

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Section: Resultsmentioning

confidence: 99%

Quorum Sensing in a Methane-Oxidizing Bacterium

Puri

Schaefer

et al. 2017

J Bacteriol

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“…QS was confirmed to be one of the regulatory pathways for EPS production and biofilm formation in microbes (Hooshangi and Bentley, 2008;Marketon et al, 2003;Ruiz et al, 2008;Vu et al, 2009). A recent study showed that quorum sensing played a significant role in the formation of aerobic granules in a sequencing batch reactor, and that these granules produced more galactose than conventional flocs .…”

Section: Smp and Eps Content In Mbr And On Membrane Surfacementioning

confidence: 98%

Effect of quorum quenching on the reactor performance, biofouling and biomass characteristics in membrane bioreactors

et al. 2013

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“…Therefore, studies to define new strategies to control biofilm formation are highly relevant (Farah et al. 2005; Ruiz et al. 2008).…”

Section: Discussionmentioning

confidence: 99%

“…Pyrite and a copper concentrate were kindly provided by W. Sand and C. Demergasso, respectively, and were used to 50 g l −1 in modified 9K medium (M9K) at pH 1·5 (Demergasso et al. 2005; Ruiz et al. 2008).…”

Section: Methodsmentioning

confidence: 99%

The extremophile Acidithiobacillus ferrooxidans possesses a c-di-GMP signalling pathway that could play a significant role during bioleaching of minerals

Ruíz

Castro

Barriga

et al. 2011

Letters in Applied Microbiology

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Aims: The primary goal of this study was to characterize the existence of a functional c‐di‐GMP pathway in the bioleaching bacterium Acidithiobacillus ferrooxidans. Methods and Results: A bioinformatic search revealed that the genome sequence of At. ferrooxidans ATCC 23270 codes for several proteins involved in the c‐di‐GMP pathway, including diguanylate cyclases (DGC), phosphodiesterases and PilZ effector proteins. Overexpression in Escherichia coli demonstrated that four At. ferrooxidans genes code for proteins containing GGDEF/EAL domains with functional DGC activity. MS/MS analysis allowed the identification of c‐di‐GMP in nucleotide preparations obtained from At. ferrooxidans cells. In addition, c‐di‐GMP levels in cells grown on the surface of solid energetic substrates such as sulfur prills or pyrite were higher than those measured in ferrous iron planktonic cells. Conclusions: At. ferrooxidans possesses a functional c‐di‐GMP pathway that could play a key role in At. ferrooxidans biofilm formation during bioleaching processes. Significance and Impact of the Study: This is the first global study about the c‐di‐GMP pathway in an acidophilic bacterium of great interest for the biomining industry. It opens a new way to explore the regulation of biofilm formation by biomining micro‐organisms during the bioleaching process.

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AHL communication is a widespread phenomenon in biomining bacteria and seems to be involved in mineral-adhesion efficiency

Cited by 61 publications

References 25 publications

Quorum Sensing in a Methane-Oxidizing Bacterium

Quorum Sensing in a Methane-Oxidizing Bacterium

Effect of quorum quenching on the reactor performance, biofouling and biomass characteristics in membrane bioreactors

The extremophile Acidithiobacillus ferrooxidans possesses a c-di-GMP signalling pathway that could play a significant role during bioleaching of minerals

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