AI-2 signalling is induced by acidic shock in probiotic strains of Lactobacillus spp.

Moslehi-Jenabian, Saloomeh; Gori, Klaus; Jespersen, Lene

doi:10.1016/j.ijfoodmicro.2009.08.011

Cited by 75 publications

(50 citation statements)

References 35 publications

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“…SAH is known to be a toxic intermediate (56,57). In line with these results, the acid stress response was affected in LuxS mutants of S. mutans, S. pyogenes, and Lactobacillus spp., respectively (19,25,58).…”

Section: Discussionmentioning

confidence: 99%

Heterologous Expression of sahH Reveals That Biofilm Formation Is Autoinducer-2-independent in Streptococcus sanguinis but Is Associated with an Intact Activated Methionine Cycle

Redanz

Standar

Podbielski

et al. 2012

Journal of Biological Chemistry

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Background: AI-2 is a by-product of the LuxS-mediated reaction within the activated methionine cycle (AMC). Results:The biofilm phenotype of a S. sanguinis luxS mutant was restored by SahH expression but not by AI-2 supplementation. Conclusion:The luxS mutant biofilm phenotype is not caused by lacking AI-2 but by an AMC defect. Significance: The SahH bypass is essential for further studies on AI-2 utilizing luxS mutants.

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Section: Discussionmentioning

confidence: 99%

Heterologous Expression of sahH Reveals That Biofilm Formation Is Autoinducer-2-independent in Streptococcus sanguinis but Is Associated with an Intact Activated Methionine Cycle

Redanz

Standar

Podbielski

et al. 2012

Journal of Biological Chemistry

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“…For example, lactic acid bacteria synthesize bacteriocins depending on the cellular density, using a QS regulatory mechanism, which requires an extracellular accumulation of autoinductor peptides (AI2) that function as chemical messengers (4, 5). There are experimental evidences that the probiotic supernatants (SN), which contain antimicrobial molecules and QS signal molecules, can influence the growth and virulence features expression of some EAggEC (Enteroaggregative Escherichia coli ) clinical strains (6, 7).…”

mentioning

confidence: 99%

“…strains could elicit strong tumor necrosis factor-alpha (TNF-α) inducing activities in macrophages through Toll-like receptor 2 (16). In addition, the antimicrobial defensins, cathelicidins, eosinophil-derived neurotoxin, and AI-2 signaling compounds of Gram-positive and Gram-negative bacteria play important roles in intra- and interspecies communication (5, 27). …”

mentioning

confidence: 99%

Immunomodulatory effect of non-viable components of probiotic culture stimulated with heat-inactivatedEscherichia coliandBacillus cereuson holoxenic mice

Dițu¹,

Chifiriuc²,

Bezirtzoglou³

et al. 2014

Microbial Ecology in Health & Disease

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BackgroundCompetition of probiotic bacteria with other species from the intestinal microbiota involves different mechanisms that occur regardless of probiotics’ viability. The objective of this paper was to assess the cytokine serum levels in holoxenic mice after oral administration of non-viable components (NVC) of Enterococcus faecium probiotic culture stimulated with heat-inactivated Escherichia coli and Bacillus cereus in comparison to NVC of unstimulated E. faecium probiotic culture.MethodsProbiotic E. faecium CMGb 16 culture, grown in the presence of heat-inactivated cultures of E. coli and B. cereus CMGB 102, was subsequently separated into supernatant (SN) and heat-inactivated cellular sediment (CS) fractions by centrifugation. Each NVC was orally administered to holoxenic mice (balb C mouse strain), in three doses, given at 24 hours. Blood samples were collected from the retinal artery, at 7, 14, and 21 days after the first administration of the NVC. The serum concentrations of IL-12 and tumor necrosis factor-alpha (TNF-α) interleukins were assessed by ELISA method.ResultsAfter the oral administration of SN component obtained from the probiotic culture stimulated with heat-inactivated cultures of B. cereus CMGB 102 and E. coli O28, the serum concentrations of IL-12 were maintained higher in the samples collected at 7 and 14 days post-administration. No specific TNF-α profile could be established, depending on stimulated or non-stimulated probiotic culture, NVC fraction, or harvesting time.ConclusionThe obtained results demonstrate that non-viable fractions of probiotic bacteria, stimulated by other bacterial species, could induce immunostimulatory effects mediated by cytokines and act, therefore, as immunological adjuvants.

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“…Among dairy-relevant pathogenic bacteria, AI-2 has been found to control biofilm formation in Listeria monocytogenes and virulence in both Staphylococcus aureus and Escherichia coli (Barrios et al, 2006;Surette & Bassler, 1998;Winzer & Williams, 2001). However, AI-2 controlled behaviours are not only restricted to pathogenic bacteria but are also found among dairy-relevant starter cultures and probiotic bacteria such as acid stress regulation in Lactococcus lactis and Lactobacillus spp., respectively (Frees, Vogensen, & Ingmer, 2003;Moslehi-Jenabian, Gori, & Jespersen, 2009). To the best of our knowledge, no previous studies have focused on AI-2 controlled behaviours in smear bacteria.…”

Section: Introductionmentioning

confidence: 99%

“…More recently,Moslehi-Jenabian et al (2009) reported inductions in luxS by the uses quantitative real time polymerase chain reaction (qRT PCR) for probiotic strains of lactobacilli upon exposure to pH as low as 4.0. The observed inductions in luxS were furthermore correlated Influence of low pH (pH 5.0) on autoinducer-2 (AI-2) activity of: (A) Corynebacterium casei 44701; (B) Microbacterium gubbeenense 15944; (C) Staphylococcus equorum subsp.…”

mentioning

confidence: 99%

Autoinducer-2 activity produced by bacteria found in smear of surface ripened cheeses

Gori

Moslehi-Jenabian

Purrotti

et al. 2011

International Dairy Journal

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AI-2 signalling is induced by acidic shock in probiotic strains of Lactobacillus spp.

Cited by 75 publications

References 35 publications

Heterologous Expression of sahH Reveals That Biofilm Formation Is Autoinducer-2-independent in Streptococcus sanguinis but Is Associated with an Intact Activated Methionine Cycle

Heterologous Expression of sahH Reveals That Biofilm Formation Is Autoinducer-2-independent in Streptococcus sanguinis but Is Associated with an Intact Activated Methionine Cycle

Immunomodulatory effect of non-viable components of probiotic culture stimulated with heat-inactivatedEscherichia coliandBacillus cereuson holoxenic mice

Autoinducer-2 activity produced by bacteria found in smear of surface ripened cheeses

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