Air sampling and analytical procedures for benzidine, 3,3′-dichlorobenzidine and their salts

Abstract

The purpose of the study was the development of new air-sampling and analytical procedures for benzidine, 3,3'-dichlorobenzidine and their salts. Air is drawn through a glass-fiber filter followed by a bed of silica gel to collect these substances as either particles or vapors. The compounds are extracted from the sampler and analyzed by HPLC with sensitivities in the range of 3 micrograms/m3 for 48-L air samples. The methods were evaluated in the laboratory with test aerosol atomospheres and found to be unaffected by temperature or humidity of the sampled environment. Tests of precision, sample stability and separation from interferants indicate that the method should provide reliable results for personal monitoring procedures.

Urinary Monitoring Method of 3,3'-Dichlorobenzidine(DCB) and Its Metabolites, N-Acetyl-DCB and N,N'-Diacetyl-DCB.

“…Solvents of DCB in experimental animal studies were Tween 20/water (1:200) by Kellner et al 9) , Emulphor/ethanol/water (1:1:8) by Decad et al 8) , corn oil by Iba 10) and Iba & Thomas 13) , and propanediol/ethanol (1:1) by Zwirner-Baier & Neumann 7) . Morales, et al 11) used 0.2% triethylamine in methanol as extraction solution for air sample of benzidine, DCB, and its salts. And Roberts & Rossano 12) dissolved in 70% acetic acid after making mixtures of citric acid containing less than 1% DCB.…”

Benzidine and Benzidine Derivatives

“…Such emissions can certainly be prevented by filtering or by using wet separators. Diphenyl base concentrations in the ambient air are monitored using TLC or HPLC [29].…”

Avaliação de metodologia de coleta e analíse de solventes/misturas de solventes no ar em ambiente de trabalho: metanol em mistura MEG (Metanol 33% Etanol 60% e Gasolina 7%)

“…, ácido fluorídrico (84), metil carbamato (8S gás sulfídrico(69), monômeros de acrilato (86), benzedina, 3,3'diclorobenzedina seus sais(87), ácido acrílico (88), metilamina, metanol, etanol, isopropanol, n butanoJ, acetona, metil etil cetona(89), metilisibutilcetona, acetato de etiJa acetato de isobutila(69), éter di-n-butílico, cresóis, nitrobenzeno, fosfina estibina, quando recoberta com cianeto de mercúrio (SO) e dimetilformamida quando recoberta com dietilamina(79). Impinger e borbulhadores com solventes ou soluções reagentes são utilizados, apesar de sua inconveniência para a coleta na zona de respiração do trabalhador.…”