Akkermansia muciniphila Ameliorates Clostridioides difficile Infection in Mice by Modulating the Intestinal Microbiome and Metabolites

Wu, Zhengjie; Xu, Qiaomai; Gu, Silan; Chen, Yunbo; Lv, Longxian; Zheng, Beiwen; Wang, Qiangqiang; Wang, Kaicen; Wang, Zhen; Xia, Jiafeng; Yang, Liya; Bian, Xiaoyuan; Jiang, Xianwan; Zheng, Lisi; Li, Lanjuan

doi:10.3389/fmicb.2022.841920

Cited by 23 publications

(24 citation statements)

References 79 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sure enough, a significant decrease in the content of SCFAs was observed in the psicose group, especially the propionate and butyrate, which were strongly responsible for Muc-2 secretion. Consistent with previous findings [45,46], Akkermansia and Lactobacillus were positively associated with the expression of Muc-2 coupled with the levels of propionate and butyrate, and significantly negatively associated with inflammatory cytokines.…”

Section: Discussionsupporting

confidence: 92%

D-Psicose intake exacerbates dextran sulfate sodium-induced colitis in mice through alteration in the gut microbiota and dysfunction of mucosal barrier

Zhang¹,

Li²,

Wang³

et al. 2024

Food Science and Human Wellness

View full text Add to dashboard Cite

D-Psicose, as a low-calorie rare sugar, has attracted a lot of attention in recent years for alternating to sucrose. The anti-obesity effect of D-psicose has been extensively confirmed in previous studies, however, the impact of D-psicose on colitis remains vague. Here, we firstly evaluated the effect of the D-psicose prophylactic intervention on dextran sulfate sodium-induced colitis in C57BL/6 mice. The pathological symptoms, inflammatory cytokines levels, gut microbiota composition, short chain fatty acids (SCFAs) production and colonic barrier integrity were comprehensively evaluated. The results confirmed that D-psicose intervention aggravated colitis, characterized by the exacerbation of colon shortening, increase of colonic inflammatory infiltration, and marked exaltation of disease activity indices and IL-6, IL-1β and TNF-α levels. Further, the dysfunction of gut microbiota was identified in the psicose group. The abundance of pro-inflammatory bacteria Lachnospiraceae_NK4A136_group was significantly up-regulated while the abundance of probiotics Akkermansia and Lactobacillus were significantly down-regulated in the psicose group compared to the model group. Moreover, the production of SCFAs was suppressed in the psicose group, accompanied by a decrease in the level of mucin 2 (Muc-2). Collectively, the underlying mechanism of the exacerbation of colitis by D-psicose intervention might be attributed to microbiota dysfunction accompanied by the reduction of SCFAs, which leads to the damage of the mucosal barrier and the intensification of inflammatory invasion.

show abstract

Section: Discussionsupporting

confidence: 92%

D-Psicose intake exacerbates dextran sulfate sodium-induced colitis in mice through alteration in the gut microbiota and dysfunction of mucosal barrier

Zhang¹,

Li²,

Wang³

et al. 2024

Food Science and Human Wellness

View full text Add to dashboard Cite

show abstract

“…Sure enough, a significant decrease in the content of SCFAs was observed in the Psicose group, especially the propionate and butyrate, which were strongly responsible for Muc-2 secretion. Consistent with previous findings 42,43 , Akkermansia and Lactobacillus were positively associated with the expression of Muc-2 coupled with the levels of propionate and butyrate, and significantly negatively associated with inflammatory cytokines.…”

Section: Spearman Correlation Analysissupporting

confidence: 92%

D-Psicose intake exacerbates DSS-induced colitis in mice through alteration in the gut microbiota and dysfunction of mucosal barrier

Wang

Zhang

et al. 2022

Preprint

View full text Add to dashboard Cite

D-Psicose, as a low-calorie rare sugar, has attracted a lot of attention in recent years for alternating to sucrose. The anti-obesity effect of D-Psicose has been extensively confirmed in previous studies, however, the impact of D-Psicose on colitis remains vague. Here, we firstly evaluated the effect of 21 days of D-Psicose prophylactic intervention on DSS-induced colitis in C57BL/6 mice. The pathological symptoms, inflammatory cytokines levels, gut microbiota composition, short chain fatty acids production and colonic barrier integrity were comprehensively evaluated. The results confirmed that D-Psicose intervention aggravated colitis, characterized by the exacerbation of colon shortening, increase of colonic inflammatory infiltration, and marked exaltation of disease activity indices and IL-6, IL-1β and TNF-α levels. Further, the dysfunction of gut microbiota was identified in the Psicose group. The abundance of pro-inflammatory bacteria Lachnospiraceae_NK4A136_group was significantly up-regulated while the abundance of probiotics Akkermansia and Lactobacillus were significantly down-regulated in the Psicose group compared to the Model group. Moreover, the production of short chain fatty acids was suppressed in the Psicose group, accompanied by a decrease in the level of Muc-2. Collectively, the underlying mechanism of the exacerbation of colitis by D-Psicose intervention might be attributed to microbiota dysfunction accompanied by the reduction of short chain fatty acids, which leads to the damage of the mucosal barrier and the intensification of inflammatory invasion.

show abstract

“…persisted. Preventive use of frequent administration of high doses of A. muciniphila has been shown to alleviate CDI-associated symptoms in a similar mouse model (40) ,which points in the direction of a co-existence rather than a symbiosis between C. difficile and mucin-degrading bacteria.…”

Section: Discussionmentioning

confidence: 99%

Overcoming donor variability and risks associated with fecal microbiota transplants through bacteriophage-mediated treatments

Rasmussen

Förster

Larsen

et al. 2023

Preprint

View full text Add to dashboard Cite

Fecal microbiota transplantation (FMT) from a healthy donor to recurrent C. difficile infection (CDI) patients has proven efficient in curing the disease, possibly through bacteriophage-mediated (phages) modulation of the gut microbiome landscape. Fecal virome transplantation (FVT, sterile filtrated donor feces) has also been shown efficient for treating the disease. FVT has the advantage over FMT that no bacteria are transferred, but FVT does not exclude the risk of transferring eukaryotic viruses. We aimed to develop methodologies to obtain safer FVT by removing and/or inactivating eukaryotic viruses, while maintaining an active phage community. Donor feces were used as inoculum for a chemostat-fermentation to remove eukaryotic viruses by dilution (FVT-ChP). FVT solutions underwent solvent-detergent treatment to inactivate enveloped viruses (FVT-SDT) and pyronin-Y treatment to block the replication of RNA-viruses (FVT-PyT). The efficacy of these treatments was assessed in a CDI mouse model and compared with untreated FVT (FVT-UnT), FMT, and saline-treatment as controls. Intriguingly, 8/8 mice receiving FVT-SDT did not reach the humane endpoints until planned euthanization and expressed limited symptoms of CDI. While 5/7 saline treated mice reached the humane endpoint. Compared to the saline treatment, lower C. difficile abundance (p<0.005) in the FVT-SDT-treated mice suggested that the intervention had hampered C. difficile colonization. The mice receiving FVT-ChP and FVT-UnT tended to express alleviated CDI symptoms compared to the saline control. This proof-of-concept study may constitute the initial step of developing therapeutic tools that targets a broad spectrum of gut-related diseases and thereby substituting FMT with a safer phage-mediated therapies.

show abstract

Akkermansia muciniphila Ameliorates Clostridioides difficile Infection in Mice by Modulating the Intestinal Microbiome and Metabolites

Cited by 23 publications

References 79 publications

D-Psicose intake exacerbates dextran sulfate sodium-induced colitis in mice through alteration in the gut microbiota and dysfunction of mucosal barrier

D-Psicose intake exacerbates dextran sulfate sodium-induced colitis in mice through alteration in the gut microbiota and dysfunction of mucosal barrier

D-Psicose intake exacerbates DSS-induced colitis in mice through alteration in the gut microbiota and dysfunction of mucosal barrier

Overcoming donor variability and risks associated with fecal microbiota transplants through bacteriophage-mediated treatments

Contact Info

Product

Resources

About