Albumin and Hydroxyethyl Starch Modulate Oxidative Inflammatory Injury to Vascular Endothelium

Lang, Janet M.; Figueroa, Mario; Chumley, Phillip; Aslan, Mutay; Hurt, John J.; Tarpey, Margaret M.; Álvarez, Beatriz; Radí, Rafael; Freeman, Bruce Α.

doi:10.1097/00000542-200401000-00012

Cited by 76 publications

(37 citation statements)

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“…36 The duration of the antiinflammatory effect after HES administration thus may be largely determined by the intravascular elimination of the colloid. 36 Contrary to these findings, no effect or an apparent proinflammatory action after HES administration has recently be shown by Lang et al 37 Additional studies evaluating the antiinflammatory effect of high-molecular-weight low-substituted HES solutions due to their probable longer intravascular confinement thus seem to be worthwhile.…”

Section: Discussionmentioning

confidence: 91%

Effect of High- and Low-molecular-weight Low-substituted Hydroxyethyl Starch on Blood Coagulation during Acute Normovolemic Hemodilution in Pigs

Thyes¹,

Madjdpour²,

Frascarolo³

et al. 2006

Anesthesiology

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Background: Hydroxyethyl starches (HES) with lower impact on blood coagulation but longer intravascular persistence are of clinical interest. The current study aimed to investigate in vivo the isolated effect of molecular weight on blood coagulation during progressive acute normovolemic hemodilution.Methods: Twenty-four pigs were normovolemically hemodiluted up to a total exchange of 50 ml ⅐ kg ؊1 ⅐ body weight ؊1 of

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Section: Discussionmentioning

confidence: 91%

Effect of High- and Low-molecular-weight Low-substituted Hydroxyethyl Starch on Blood Coagulation during Acute Normovolemic Hemodilution in Pigs

Thyes¹,

Madjdpour²,

Frascarolo³

et al. 2006

Anesthesiology

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“…Last, albumin has protective effects on the endothelium. For example, it can inhibit the binding of polymorphonuclear leukocytes to cultured endothelial cells (10).…”

Section: Human Serum Albuminmentioning

confidence: 99%

Oxidation of the albumin thiol to sulfenic acid and its implications in the intravascular compartment

Turell

Carballal

Botti

et al. 2009

Braz J Med Biol Res

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Human serum albumin (HSA) is the most abundant protein in the intravascular compartment. It possesses a single thiol, Cys34, which constitutes ~8 0% of the total thiols in plasma. This thiol is able to scavenge plasma oxidants. A central intermediate in this potential antioxidant activity of human serum albumin is sulfenic acid (HSA-SOH). Work from our laboratories has demonstrated the formation of a relatively stable sulfenic acid in albumin through complementary spectrophotometric and mass spectrometric approaches. Recently, we have been able to obtain quantitative data that allowed us to measure the rate constants of sulfenic acid reactions with molecules of analytical and biological interest. Kinetic considerations led us to conclude that the most likely fate for sulfenic acid formed in the plasma environment is the reaction with low molecular weight thiols to form mixed disulfides, a reversible modification that is actually observed in ~2 5% of circulating albumin. Another possible fate for sulfenic acid is further oxidation to sulfinic and sulfonic acids. These irreversible modifications are also detected in the circulation. Oxidized forms of albumin are increased in different pathophysiological conditions and sulfenic acid lies in a mechanistic junction, relating oxidizing species to final thiol oxidation products.

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“…The latter include salutary interactions with vascular endothelium, 6 platelet antiaggregatory effects, 7 antagonism of erythrocyte sedimentation in low-flow states, 8 reaction with nitric oxide to form a stable S-nitrosothiol with endothelium-derived relaxing factor-like properties, 9 and antagonism of the binding of activated neutrophils to endothelial cells in response to inflammatory stimuli. 10 In the ALIAS pilot clinical trial, subjects who received thrombolysis (IV tissue plasminogen activator) plus high-dose albumin were twice as likely to attain a favorable neurological outcome at 3 months than did tissue plasminogen activator-treated subjects who received lower-dose albumin. 4 This result suggests that albumin therapy might act to maintain microvascular patency and retard reocclusion after thrombolysis.…”

mentioning

confidence: 99%

Albumin Therapy Improves Local Vascular Dynamics in a Rat Model of Primary Microvascular Thrombosis

Nimmagadda

Park

Prado

et al. 2008

Stroke

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Background and Purpose— High-dose human albumin is robustly neuroprotective in preclinical ischemia models and is currently in phase III clinical trial for acute ischemic stroke. To explore the hypothesis that albumin’s protective effect is mediated in part by salutary intravascular mechanisms, we assessed microvascular hemodynamics in a model of laser-induced cortical arteriolar thrombosis. Methods— The cortical microcirculation of anesthetized, physiologically monitored Sprague-Dawley rats was studied in vivo via a frontoparietal cranial window (intact dura) by two-photon laser-scanning microscopy after plasma-labeling with fluorescein-dextran. Focal thrombosis was produced in 30- to 50-μm cortical arterioles by laser irradiation. Arteriolar flow velocity was measured repeatedly by line-scanning. At 30 minutes post-thrombosis, animals were treated with either human albumin, 2 g/kg, or with saline control. Results— Baseline arteriolar flow velocity averaged 3.5±1.8 mm/s and was reduced to 10% to 13% of control values by laser-induced thrombosis, which also led to focal vasodilatation (mean, 49% above baseline diameter). Saline treatment at 30 minutes post-thrombosis failed to influence arteriolar flow velocity, which remained depressed at 10% to 22% of control throughout the subsequent 60- to 90-minute observation period. By contrast, albumin treatment induced a prompt rise in median flow velocity to 38% of control by 10 minutes post-treatment, and to 61% to 67% of control by 50 to 60 minutes. Conclusions— High-dose albumin therapy induces a prompt, sustained improvement in microvascular hemodynamics distal to a cortical arteriolar thrombosis; these data support an important intravascular component to albumin’s protective effect in acute cerebral ischemia.

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Albumin and Hydroxyethyl Starch Modulate Oxidative Inflammatory Injury to Vascular Endothelium

Abstract: Clinical human serum albumin preparations show modest intrinsic non-thiol-dependent antiinflammatory properties in vitro, a phenomenon that was not observed with hydroxyethyl starch.

Cited by 76 publications

References 0 publications

Effect of High- and Low-molecular-weight Low-substituted Hydroxyethyl Starch on Blood Coagulation during Acute Normovolemic Hemodilution in Pigs

Effect of High- and Low-molecular-weight Low-substituted Hydroxyethyl Starch on Blood Coagulation during Acute Normovolemic Hemodilution in Pigs

Oxidation of the albumin thiol to sulfenic acid and its implications in the intravascular compartment

Albumin Therapy Improves Local Vascular Dynamics in a Rat Model of Primary Microvascular Thrombosis

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