Alcohol consumption increases locomotion in an open field and induces Fos-immunoreactivity in reward and approach/withdrawal-related neurocircuitries

Wscieklica, Tatiana; Viana, Milena de Barros; Sueur-Maluf, Luciana Le; Pouza, Kathlein Cristiny Peres; Spadari, Regina Célia; Céspedes, Isabel Cristina

doi:10.1016/j.alcohol.2015.11.005

Cited by 18 publications

(8 citation statements)

References 82 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…High-drinking male rats, as compared to low drinkers, showed a significantly increased DLS Drd1 expression, as well as reliably increased VMS Egr1 and ∆FosB, with similar trends evident for Fos and FosB. Broadly consistent with our IEG activation marker findings, previous studies found higher levels of Fos in the VMS of EtOH-preferring rats [71] and of rats with a higher mean EtOH intake [72]. Fos expression was also greater in the VMS of male rats selectively bred for high EtOH consumption low-consuming rats [73].…”

Section: Discussionsupporting

confidence: 90%

Effects of the Phosphodiesterase 10A Inhibitor MR1916 on Alcohol Self-Administration and Striatal Gene Expression in Post-Chronic Intermittent Ethanol-Exposed Rats

Bertotto,

Lampson-Stixrud,

Sinha

et al. 2024

Cells

View full text Add to dashboard Cite

Alcohol use disorder (AUD) requires new neurobiological targets. Problematic drinking involves underactive indirect pathway medium spiny neurons (iMSNs) that subserve adaptive behavioral selection vs. overactive direct pathway MSNs (dMSNs) that promote drinking, with a shift from ventromedial to dorsolateral striatal (VMS, DLS) control of EtOH-related behavior. We hypothesized that inhibiting phosphodiesterase 10A (PDE10A), enriched in striatal MSNs, would reduce EtOH self-administration in rats with a history of chronic intermittent ethanol exposure. To test this, Wistar rats (n = 10/sex) with a history of chronic intermittent EtOH (CIE) vapor exposure received MR1916 (i.p., 0, 0.05, 0.1, 0.2, and 0.4 µmol/kg), a PDE10A inhibitor, before operant EtOH self-administration sessions. We determined whether MR1916 altered the expression of MSN markers (Pde10a, Drd1, Drd2, Penk, and Tac1) and immediate-early genes (IEG) (Fos, Fosb, ΔFosb, and Egr1) in EtOH-naïve (n = 5–6/grp) and post-CIE (n = 6–8/grp) rats. MR1916 reduced the EtOH self-administration of high-drinking, post-CIE males, but increased it at a low, but not higher, doses, in females and low-drinking males. MR1916 increased Egr1, Fos, and FosB in the DLS, modulated by sex and alcohol history. MR1916 elicited dMSN vs. iMSN markers differently in ethanol-naïve vs. post-CIE rats. High-drinking, post-CIE males showed higher DLS Drd1 and VMS IEG expression. Our results implicate a role and potential striatal bases of PDE10A inhibitors to influence post-dependent drinking.

show abstract

Section: Discussionsupporting

confidence: 90%

Effects of the Phosphodiesterase 10A Inhibitor MR1916 on Alcohol Self-Administration and Striatal Gene Expression in Post-Chronic Intermittent Ethanol-Exposed Rats

Bertotto,

Lampson-Stixrud,

Sinha

et al. 2024

Cells

View full text Add to dashboard Cite

show abstract

“…; Wscieklica et al . ). In rodents, adolescence is commonly divided into three time periods: early adolescence (21–34 days postnatal), middle adolescence (34–46 days postnatal) and late adolescence (46–59 days postnatal; Spear, ; Laviola et al .…”

Section: Discussionmentioning

confidence: 97%

“…However, phenotypic plasticity implies a cost for early adaptation that can be paid during development later in life (West-Eberhard, 2005). In this context, patterns of locomotor activity were evaluated in pups at different ages (23, 45 and 60 days old) in the 'open field' , according to previous studies (Barros et al 2006;Aragão et al 2011;Hu et al 2015;Wscieklica et al 2016). In rodents, adolescence is commonly divided into three time periods: early adolescence (21-34 days postnatal), middle adolescence (34-46 days postnatal) and late adolescence (46-59 days postnatal; Spear, 2000;Laviola et al 2003).…”

Section: Discussionmentioning

confidence: 99%

Maternal voluntary physical activity attenuates delayed neurodevelopment in malnourished rats

Fragoso

Lira

Chagas

et al. 2017

Experimental Physiology

View full text Add to dashboard Cite

What is the central question of this study? In the present study, a reproducible model of maternal voluntary physical activity was developed to evaluate the adaptive response of physical activity by attenuating the effects of maternal undernutrition on physical features, reflex ontogeny and growth trajectory of offspring during development. What is the main finding and its importance? Maternal physical activity may induce neuronal maturation of sensorimotor connections impacting on the patterns of locomotor activity in malnourished offspring. Thus, physical activity should be considered as a therapeutic means of countering the effects of maternal undernutrition, by providing a useful strategy for enhancing the neuronal activity of children born to mothers who experience a restricted diet during pregnancy. This study evaluated the effects of maternal voluntary physical activity during pregnancy and lactation on somatic growth (SG), reflex ontogeny (RO) and locomotor activity (LA) of rats whose mothers were protein restricted. Virgin female Wistar rats were divided into the following six groups: control, normal protein (C-NP, n = 4); control, low protein (C-LP, n = 4); inactive, normal protein (I-NP, n = 8); inactive, low protein (I-LP, n = 7); very active, normal protein (VA-NP, n = 8); and very active, low protein (VA-LP, n = 6). Voluntary physical activity was recorded daily in dams. The LP groups were fed an 8% casein diet, whereas control groups were fed a 17% casein diet during pregnancy and lactation. Offspring were evaluated in terms of SG (body weight and length, latero-lateral skull axis and anteroposterior head axis) and RO (palmar grasp, righting, free-fall righting, negative geotaxis, cliff avoidance, auditory startle response and vibrissa placing). The LA was evaluated at 23, 45 and 60 days old in the open field. Voluntary physical activity was reduced during pregnancy and lactation independent of the maternal diet. Pups from LP dams showed delayed SG, reflex maturation and patterns of LA when compared with control pups. The C-LP and I-LP pups showed a delayed SG, RO and LA. Pups from VA-LP mothers showed no delay in SG and RO and presented a faster development of patterns of LA. Maternal voluntary physical activity attenuated the effects of LP diet on indicators of neurodevelopment and patterns of LA of offspring.

show abstract

“…Furthermore, CaMKII autophosphorylation-deficient mice demonstrate enhanced negative reinforcing actions of ethanol [25], suggesting a role for CaMKII autophosphorylation in the negative affective state observed in chronic intermittent ethanol vapor exposure (CIE) rats. In support of this hypothesis, recent studies indicate that neural activity in the ventral striatum is critical for the neurochemical effects of ethanol, which may contribute to further excessive ethanol consumption and dependence [26,27]. More notable is that reduced activity of CaMKII at its autophosphorylation site was observed in the ventral striatum, selectively in the nucleus accumbens shell region, following chronic ethanol drinking [21].…”

Section: Introductionmentioning

confidence: 92%

Individual Differences in Ethanol Drinking and Seeking Behaviors in Rats Exposed to Chronic Intermittent Ethanol Vapor Exposure is Associated with Altered CaMKII Autophosphorylation in the Nucleus Accumbens Shell

Somkuwar

Mandyam

2019

Brain Sciences

View full text Add to dashboard Cite

Chronic intermittent ethanol vapor exposure (CIE) in rodents produces reliable and high blood ethanol concentration and behavioral symptoms associated with moderate to severe alcohol use disorder (AUD)-for example, escalation of operant ethanol self-administration, a feature suggestive of transition from recreational to addictive use, is a widely replicated behavior in rats that experience CIE. Herein, we present evidence from a subset of rats that do not demonstrate escalation of ethanol self-administration following seven weeks of CIE. These low responders (LR) maintain low ethanol self-administration during CIE, demonstrate lower relapse to drinking during abstinence and reduced reinstatement of ethanol seeking triggered by ethanol cues when compared with high responders (HR). We examined the blood ethanol levels in LR and HR rats during CIE and show higher levels in LR compared with HR. We also examined peak corticosterone levels during CIE and show that LR rats have higher levels compared with HR rats. Lastly, we evaluated the levels of Ca 2+ /calmodulin-dependent protein kinase II (CaMKII) in the nucleus accumbens shell and reveal that the activity of CaMKII, which is autophosphorylated at site Tyr-286, is significantly reduced in HR rats compared with LR rats. These findings demonstrate that dysregulation of the hypothalamic-pituitary-adrenal axis activity and plasticity-related proteins regulating molecular memory in the nucleus accumbens shell are associated with higher ethanol-drinking and -seeking in HR rats. Future mechanistic studies should evaluate CaMKII autophosphorylation-dependent remodeling of glutamatergic synapses in the ventral striatum as a plausible mechanism for the CIE-induced enhanced ethanol drinking and seeking behaviors.

show abstract

Alcohol consumption increases locomotion in an open field and induces Fos-immunoreactivity in reward and approach/withdrawal-related neurocircuitries

Cited by 18 publications

References 82 publications

Effects of the Phosphodiesterase 10A Inhibitor MR1916 on Alcohol Self-Administration and Striatal Gene Expression in Post-Chronic Intermittent Ethanol-Exposed Rats

Effects of the Phosphodiesterase 10A Inhibitor MR1916 on Alcohol Self-Administration and Striatal Gene Expression in Post-Chronic Intermittent Ethanol-Exposed Rats

Maternal voluntary physical activity attenuates delayed neurodevelopment in malnourished rats

Individual Differences in Ethanol Drinking and Seeking Behaviors in Rats Exposed to Chronic Intermittent Ethanol Vapor Exposure is Associated with Altered CaMKII Autophosphorylation in the Nucleus Accumbens Shell

Contact Info

Product

Resources

About