Alcohol-fixed specimens for high-contrast post-mortem MRI

Wereszczyńska, Beata

doi:10.1016/j.fri.2021.200449

Cited by 2 publications

(3 citation statements)

References 42 publications

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“…However, a number of considerations should be taken into account before deciding on the sample preparation method and scanning order. Dehydration of the sample with ethanol increases the contrast for X-ray imaging, but may affect the MR images 62 . The majority of MR techniques are highly dependent on water content.…”

Section: Mri Imagingmentioning

confidence: 99%

Preparation of large biological samples for high-resolution, hierarchical, synchrotron phase-contrast tomography with multimodal imaging compatibility

et al. 2023

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Imaging across different scales is essential for understanding healthy organ morphology and pathophysiological changes. The macro-and microscale three-dimensional morphology of large samples, including intact human organs, is possible with X-ray microtomography (using laboratory or synchrotron sources). Preparation of large samples for high-resolution imaging, however, is challenging due to limitations such as sample shrinkage, insufficient contrast, movement of the sample and bubble formation during mounting or scanning. Here, we describe the preparation, stabilization, dehydration and mounting of large soft-tissue samples for X-ray microtomography. We detail the protocol applied to whole human organs and hierarchical phase-contrast tomography at the European Synchrotron Radiation Facility, yet it is applicable to a range of biological samples, including complete organisms. The protocol enhances the contrast when using X-ray imaging, while preventing sample motion during the scan, even with different sample orientations. Bubbles trapped during mounting and those formed during scanning (in the case of synchrotron X-ray imaging) are mitigated by multiple degassing steps. The sample preparation is also compatible with magnetic resonance imaging, computed tomography and histological observation. The sample preparation and mounting require 24-36 d for a large organ such as a whole human brain or heart. The preparation time varies depending on the composition, size and fragility of the tissue. Use of the protocol enables scanning of intact organs with a diameter of 150 mm with a local voxel size of 1 μm. The protocol requires users with expertise in handling human or animal organs, laboratory operation and X-ray imaging.

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Section: Mri Imagingmentioning

confidence: 99%

Preparation of large biological samples for high-resolution, hierarchical, synchrotron phase-contrast tomography with multimodal imaging compatibility

et al. 2023

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show abstract

“…However, a number of considerations should be taken into account before deciding on the sample preparation method and scanning order. Dehydration of the sample with ethanol increases the contrast for X-ray imaging, but significantly affects the MR images 52 . The majority of MR techniques are highly dependent on water content.…”

Section: Mri Imagingmentioning

confidence: 99%

“…Dehydration of the sample with ethanol increases the contrast for X-ray imaging, but significantly affects the MR images 52 . The majority of MR techniques are highly dependent on water content.…”

Section: Introductionmentioning

confidence: 99%

Preparation of large biological samples for high-resolution, hierarchical, multi-modal imaging

Brunet

Walsh

Wagner

et al. 2022

Preprint

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Imaging the different scales of biological tissue is essential for understanding healthy organ behavior and pathophysiological changes. X-ray micro-tomography using both laboratory (μCT) and synchrotron sources (sCT) is a promising tool to image the 3D morphology at the macro- and micro-scale of large samples, including intact human organs. Preparation of large samples for high resolution imaging techniques remains a challenge due to limitations with current methods, such as sample shrinkage, insufficient contrast, movement of the sample and bubble formation during mounting or scanning. Here, we describe a protocol to prepare, stabilize, and image large soft-tissue samples with X-ray microtomography. We demonstrate the protocol using intact human organs and Hierarchical Phase-Contrast Tomography (HiP-CT) imaging at the European Synchrotron Radiation Facility, but the protocol is equally applicable to a range of biological samples, including complete organisms, for both laboratory and synchrotron source tomography. Our protocol enhances the contrast of the sample, while preventing sample motion during the scan, even in case of different sample orientations. Bubbles trapped during mounting and those formed during scanning (in case of synchrotron X-ray imaging) are mitigated by multiple degassing steps. The sample preparation is also compatible with magnetic resonance imaging (MRI), CT, and histological observation. We describe a protocol for sample preparation and mounting which requires 25 to 34 days for a large organ such as an intact human brain or heart. This preparation time varies depending on the composition, size, and fragility of the tissue. Use of the protocol enables scanning of intact organs with a diameter of 150 mm with a local pixel size of one micron using HiP-CT.

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Alcohol-fixed specimens for high-contrast post-mortem MRI

Cited by 2 publications

References 42 publications

Preparation of large biological samples for high-resolution, hierarchical, synchrotron phase-contrast tomography with multimodal imaging compatibility

Preparation of large biological samples for high-resolution, hierarchical, synchrotron phase-contrast tomography with multimodal imaging compatibility

Preparation of large biological samples for high-resolution, hierarchical, multi-modal imaging

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