Aldehyde dehydrogenase isoforms and inflammatory cell populations are differentially expressed in term human placentas affected by intrauterine growth restriction

Chu, Alison; Najafzadeh, Parisa; Sullivan, Peggy; Cone, Brian; Elshimali, Ryan; Shakeri, Hania; Janzen, Carla; Mah, Vei; Wadehra, Madhuri

doi:10.1016/j.placenta.2019.03.015

Cited by 15 publications

(12 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Thus, the consumption of higher levels of soybean oil could improve the rate of lipid oxidation, accumulation of free radicals into the cells causing cell damage and, consequently, the inflammatory condition that would lead to metabolic diseases such as type 2 diabetes and atherosclerosis [53]. These results confirm previous studies that identified the fatty aldehyde dehydrogenase isozymes, such as AL3A2, have cell-specific functions associated with inflammation, differentiation, or oxidative-stress responses [54,55].…”

Section: Different Levels Of Dietary Soybean Oil Modulate Gene Expres...supporting

confidence: 84%

Differential Gene Expression Associated with Soybean Oil Level in the Diet of Pigs

Fanalli

Silva

Gomes

et al. 2022

Animals

View full text Add to dashboard Cite

The aim of this study was to identify the differentially expressed genes (DEG) from the skeletal muscle and liver samples of animal models for metabolic diseases in humans. To perform the study, the fatty acid (FA) profile and RNA sequencing (RNA-Seq) data of 35 samples of liver tissue (SOY1.5, n = 17 and SOY3.0, n = 18) and 36 samples of skeletal muscle (SOY1.5, n = 18 and SOY3.0, n = 18) of Large White pigs were analyzed. The FA profile of the tissues was modified by the diet, mainly those related to monounsaturated (MUFA) and polyunsaturated (PUFA) FA. The skeletal muscle transcriptome analysis revealed 45 DEG (FDR 10%), and the functional enrichment analysis identified network maps related to inflammation, immune processes, and pathways associated with oxidative stress, type 2 diabetes, and metabolic dysfunction. For the liver tissue, the transcriptome profile analysis revealed 281 DEG, which participate in network maps related to neurodegenerative diseases. With this nutrigenomics study, we verified that different levels of soybean oil in the pig diet, an animal model for metabolic diseases in humans, affected the transcriptome profile of skeletal muscle and liver tissue. These findings may help to better understand the biological mechanisms that can be modulated by the diet.

show abstract

Section: Different Levels Of Dietary Soybean Oil Modulate Gene Expres...supporting

confidence: 84%

Differential Gene Expression Associated with Soybean Oil Level in the Diet of Pigs

Fanalli

Silva

Gomes

et al. 2022

Animals

View full text Add to dashboard Cite

show abstract

“…Edaravone is an established free radical scavenger which has a hydroxyl radical quenching effect and an inhibitory effect against peroxynitrite and peroxyl radicals [ 53 ]. One of the important factors leading to fetal growth restriction in preeclampsia is the increased production of placental oxidants due to increased maternal and fetal metabolism [ 10 , 48 ]. The role of antioxidants in management of placental ischemia-induced growth retardation and decreased placental weight has been investigated [ 36 , 43 , 52 ].…”

Section: Discussionmentioning

confidence: 99%

Effect of edaravone on pregnant mice and their developing fetuses subjected to placental ischemia

Atallah

Yamashita

Abe

2021

Reprod Biol Endocrinol

View full text Add to dashboard Cite

Growing evidence indicates that reduced uterine perfusion pressure (RUPP) triggers the cascade of events leading to preeclampsia. Edaravone is a powerful free radical scavenger used for the treatment of ischemia/reperfusion diseases due to its anti-oxidative stress and anti-inflammatory properties. Here we investigate the effect of edaravone (3 mg/kg) on different maternal and fetal outcomes of RUPP-induced placental ischemia mice model. RUPP surgery was performed on gestation day (GD) 13 followed by edaravone injection from GD14 to GD18, sacrifice day. The results showed that edaravone injection significantly decreased the maternal blood pressure (113.2 ± 2.3 mmHg) compared with RUPP group (131.5 ± 1.9 mmHg). Edaravone increased fetal survival rate (75.4%) compared with RUPP group (54.4%), increased fetal length, weights, and feto-placental ratio (7.2 and 5.7 for RUPP and RUPP-Edaravone groups, respectively) compared with RUPP group. In addition, RUPP resulted in many fetal morphological abnormalities as well as severe delayed ossification, however edaravone decreased the morphological abnormalities and increased the ossification of the fetal endoskeleton. Edaravone improved the histopathological structure of the maternal kidney and heart as well as decreased the elevated blood urea and creatinine levels (31.5 ± 0.15 mg/dl (RUPP), 25.6 ± 0.1 mg/dl (RUPP+edaravone) for urea and 5.4 ± 0.1 mg/dl (RUPP), 3.5 ± 0.1 mg/dl (RUPP+edaravone) for creatinine) and decreased cleaved caspase-3 expression in the maternal kidney. In conclusion, this study demonstrated that our RUPP mice model recapitulated preeclampsia symptoms and edaravone injection ameliorated most of these abnormalities suggesting its effectiveness and potential application in preeclampsia treatment regimes.

show abstract

“…The significantly down-regulated gene HLA–DQA1 encodes a membrane-anchored protein for presenting peptides derived from extracellular proteins. It is mainly expressed in lymphocytes, dendritic cells, and macrophages; these antigen-presenting cells show altered plasticity in FGR placentas ( Freitag et al, 2014 ; Liu et al, 2015 ; Chu et al, 2019 ). In addition, HLA–DQA1 was involved in 8 of the top 10 KEGG pathways (see Supplementary Table 3 ), indicating that it is closely associated with FGR and fetal programming via immunologic processes.…”

Section: Discussionmentioning

confidence: 99%

Integrated Analysis of Key Genes and Pathways Involved in Fetal Growth Restriction and Their Associations With the Dysregulation of the Maternal Immune System

et al. 2021

View full text Add to dashboard Cite

Fetal growth restriction (FGR) is a common pregnancy complication and a risk factor for infant death. Most patients with FGR have preeclampsia, gestational diabetes mellitus, or other etiologies, making it difficult to determine the specific molecular mechanisms underlying FGR. In this study, an integrated analysis was performed using gene expression profiles obtained from Gene Expression Omnibus. Differentially expressed genes (DEGs) between healthy and FGR groups were screened and evaluated by functional enrichment and network analyses. In total, 80 common DEGs (FDR < 0.05) and 17 significant DEGs (FDR < 0.005) were screened. These genes were enriched for functions in immune system dysregulation in the placenta based on Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses. Among hub genes identified as candidates for FGR and fetal reprogramming, LEP, GBP5, HLA–DQA1, and CTGF were checked by quantitative polymerase chain reaction, immunohistochemistry, and western blot assays in placental tissues. Immune imbalance could cause hypoxia environment in placenta tissues, thus regulating the fetal-reprogramming. A significant association between CTGF and HIF-1α levels was confirmed in placenta tissues and HTR8 cells under hypoxia. Our results suggest that an immune imbalance in the placenta causes FGR without other complications. We provide the first evidence for roles of CTGF in FGR and show that CTGF may function via HIF-1α-related pathways. Our findings elucidate the pathogenesis of FGR and provide new therapeutic targets.

show abstract

Aldehyde dehydrogenase isoforms and inflammatory cell populations are differentially expressed in term human placentas affected by intrauterine growth restriction

Cited by 15 publications

References 40 publications

Differential Gene Expression Associated with Soybean Oil Level in the Diet of Pigs

Differential Gene Expression Associated with Soybean Oil Level in the Diet of Pigs

Effect of edaravone on pregnant mice and their developing fetuses subjected to placental ischemia

Integrated Analysis of Key Genes and Pathways Involved in Fetal Growth Restriction and Their Associations With the Dysregulation of the Maternal Immune System

Contact Info

Product

Resources

About