Aldose Reductase Inhibitory Potential and Anti-cataract Activity of Punica granatum Fruit Extract

Mestry, Snehal N.; Bhatkhande, Gauri; Dhodi, Jayesh B.; Juvekar, Archana R.

doi:10.1016/j.curtheres.2016.05.004

Cited by 1 publication

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“…Normally, 3% glucose enters in the polyol pathway (Morrison et al 1970), but under the hyperglycemic condition, it goes to more than 30%, resulting in several metabolic imbalances of the cell (Gonzalez et al 1984). Excess accumulation of sorbitol in the lens fibers causes the influx of water and generates osmotic swelling resulting into cataracts and progression of diabetic complications (Kinoshita et al 1981;Mestry et al 2016). Advances in understanding the pathological roles of aldose reductase revealed that this enzyme is also involved in inflammatory signaling under both normoglycemic and hyperglycemic conditions.…”

Section: Introductionmentioning

confidence: 99%

Phytochemical profile, aldose reductase inhibitory, and antioxidant activities of Indian traditional medicinal Coccinia grandis (L.) fruit extract

Kondhare

Lade

2017

3 Biotech

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(L.) fruits (CGFs) are commonly used for culinary purposes and has several therapeutic applications in the Southeast Asia. The aim of this work was to evaluate phytochemical profile, aldose reductase inhibitory (ARI), and antioxidant activities of CGF extract. The CGFs were extracted with different solvents including petroleum ether, dichloromethane, acetone, methanol, and water. The highest yield of total extractable compounds (34.82%) and phenolic content (11.7 ± 0.43 mg of GAE/g dried extract) was found in methanol extract, whereas water extract showed the maximum content of total flavonoids (82.8 ± 7.8 mg QE/g dried extract). Gas chromatography-mass spectroscopy (GC-MS) analysis of methanol and water extract revealed the presence of flavonoids, phenolic compounds, alkaloids, and glycosides in the CGFs. Results of the in vitro ARI activity against partially purified bovine lens aldose reductase showed that methanol extract of CGFs exhibited 96.6% ARI activity at IC value 6.12 µg/mL followed by water extract 89.1% with the IC value 6.50 µg/mL. In addition, methanol and water extracts of CGF showed strong antioxidant activities including ABTS scavenging, DPPH* scavenging, and hydroxyl radical scavenging. Our results suggest that high percentage of both flavonoids and phenolic contents in the CGFs are correlated with the ARI and antioxidant activities. The fruits of are thus potential bifunctional agents with ARI and antioxidant activities that can be used for the prevention and management of DM and associated diseases.

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