Aldosterone is not Involved in the Ventricular Remodeling Process Induced by Tobacco Smoke Exposure

Santos, Priscila P.; Nogueira, Bruna F.; Rafacho, Bruna Paola Murino; Azevedo, Paula Schmidt; Polegato, Bertha Furlan; Chiuso-Minicucci, Fernanda; Bonomo, Camila; Roscani, Meliza Goi; Zorzella-Pezavento, Sofia Fernanda Gonçalves; Tanni, Suzana Erico; Pereira, Elenize Jamas; Okoshi, Marina Politi; Paiva, Sérgio Alberto Rupp de; Zornoff, Leonardo A. M.; Minicucci, Marcos F.

doi:10.1159/000343309

Cited by 6 publications

(5 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Regardless of the complexity of the remodeling process, the term is frequently used as a synonym for ventricular enlargement associated with functional alterations after MI [18-21]. Indeed, in experimental models, some, but not all studies showed attenuation of left ventricular dimensions with aldosterone receptor blockade [22-24]. The same fact was observed in clinical trials [25-27].…”

Section: Discussionmentioning

confidence: 99%

Mechanisms Involved in the Beneficial Effects of Spironolactone after Myocardial Infarction

et al. 2013

Self Cite

View full text Add to dashboard Cite

IntroductionOur objective was to analyze the effect of spironolactone on cardiac remodeling after experimental myocardial infarction (MI), assessed by matricellular proteins levels, cardiac collagen amount and distribution, myocardial tissue metalloproteinase inhibitor-1(TIMP-1) concentration, myocyte hypertrophy, left ventricular architecture, and in vitro and in vivo cardiac function.MethodsWistar rats were assigned to 4 groups: control group, in which animals were submitted to simulated surgery (SHAM group; n=9); group that received spironolactone and in which animals were submitted to simulated surgery (SHAM-S group, n=9); myocardial infarction group, in which animals were submitted to coronary artery ligation (MI group, n=15); and myocardial infarction group with spironolactone supplementation (MI-S group, n=15). The rats were observed for 3 months.ResultsThe MI group had higher values of left cardiac chambers and mass index and lower relative wall thicknesses compared with the SHAM group. In addition, diastolic and systolic functions were worse in the MI groups. However, spironolactone did not influence any of these variables. The MI-S group had a lower myocardial hydroxyproline concentration and myocyte cross-sectional area compared with the MI group. Myocardial periostin and collagen type III were lower in the MI-S group compared with the MI-group. In addition, TIMP-1 concentration in myocardium was higher in the MI-S group compared with the MI group.ConclusionsThe predominant consequence of spironolactone supplementation after MI is related to reductions in collagens, with discrete attenuation of other remodeling variables. Importantly, this effect may be modulated by periostin and TIMP-1 levels.

show abstract

Section: Discussionmentioning

confidence: 99%

Mechanisms Involved in the Beneficial Effects of Spironolactone after Myocardial Infarction

et al. 2013

Self Cite

View full text Add to dashboard Cite

show abstract

“…MMP-2 was analyzed as per Tyagi et al [24,25]. In brief, samples were diluted in extraction buffer with 50 mM Tris pH 7.4, 0.2 M NaCl, 0.1 % Triton X, and 10 mM CaCl 2 .…”

Section: Methodsmentioning

confidence: 99%

Acute Doxorubicin-Induced Cardiotoxicity is Associated with Matrix Metalloproteinase-2 Alterations in Rats

Polegato

Minicucci

Azevedo

et al. 2015

Cell Physiol Biochem

Self Cite

View full text Add to dashboard Cite

Background: Doxorubicin can cause cardiotoxicity. Matrix metalloproteinases (MMP) are responsible for degrading extracellular matrix components which play a role in ventricular dilation. Increased MMP activity occurs after chronic doxorubicin treatment. In this study we evaluated in vivo and in vitro cardiac function in rats with acute doxorubicin treatment, and examined myocardial MMP and inflammatory activation, and gene expression of proteins involved in myocyte calcium transients. Methods: Wistar rats were injected with doxorubicin (Doxo, 20 mg/kg) or saline (Control). Echocardiogram was performed 48 h after treatment. Myocardial function was assessed in vitro in Langendorff preparation. Results: In left ventricle, doxorubicin impaired fractional shortening (Control 0.59±0.07; Doxo 0.51±0.05; p<0.001), and increased isovolumetric relaxation time (Control 20.3±4.3; Doxo 24.7±4.2 ms; p=0.007) and myocardial passive stiffness. MMP-2 activity, evaluated by zymography, was increased in Doxo (Control 141338 ± 8924; Doxo 188874 ± 7652 arbitrary units; p<0.001). There were no changes in TNF-α, INF-γ, IL-10, and ICAM-1 myocardial levels. Expression of phospholamban, Serca-2a, and ryanodine receptor did not differ between groups. Conclusion: Acute doxorubicin administration induces in vivo left ventricular dysfunction and in vitro increased myocardial passive stiffness in rats. Cardiac dysfunction is related to myocardial MMP-2 activation. Increased inflammatory stimulation or changed expression of the proteins involved in intracellular calcium transients is not involved in acute cardiac dysfunction.

show abstract

“…A two-dimensional parasternal short-axis view of the left ventricle (LV) was obtained at the level of the papillary muscles. M-mode tracings were obtained from short-axis views of the LV at or just below the tip of the mitral-valve leaflets, and at the level of the aortic valve and left atrium [29,30,31,32]. M-mode images of the LV were printed on a black-and-white thermal printer (Sony UP-890MD) at a sweep speed of 100 mm/s.…”

Section: Methodsmentioning

confidence: 99%

Aldosterone Blockade Reduces Mortality without Changing Cardiac Remodeling in Spontaneously Hypertensive Rats

Cezar

Damatto

Martinez

et al. 2013

Cell Physiol Biochem

Self Cite

View full text Add to dashboard Cite

Background: The role of aldosterone blockers during transition from long-term compensated hypertrophy to dilated failure is not completely understood. In this study we evaluated the effects of early administration of spironolactone on cardiac remodeling, myocardial function, and mortality in spontaneously hypertensive rats (SHR). Methods: Sixteen-month-old SHR received no treatment (SHR-C, n=72) or spironolactone (SHR-SPR, 20 mg/kg/day, n=34) for six months. Echocardiogram was performed before and after treatment. Myocardial function was analyzed in left ventricular (LV) papillary muscle preparations. Myocardial collagen and hydroxyproline concentration were evaluated by morphometry and spectrophotometry, respectively. LV gene expression was assessed by real time RT-PCR. Statistics: Student's t test; Log rank test (Kaplan Meyer). Results: SHR-C and SHR-SPR presented mortality rates of 71 and 38%, respectively (p=0.004). Systolic arterial pressure did not differ between groups (SHR-C 199±43; SHR-SPR 200±35 mmHg). Initial and final echocardiograms did not show significant differences in cardiac structures or LV function between groups. Myocardial function was similar between groups at basal and after inotropic stimulation. Collagen fractional area, hydroxyproline concentration, gene expression for α- and β-myosin heavy chain, atrial natriuretic peptide, and Serca2a were not different between groups. Conclusion: Early spironolactone administration reduces mortality without changing cardiac remodeling in spontaneous hypertensive rats.

show abstract

Aldosterone is not Involved in the Ventricular Remodeling Process Induced by Tobacco Smoke Exposure

Cited by 6 publications

References 34 publications

Mechanisms Involved in the Beneficial Effects of Spironolactone after Myocardial Infarction

Mechanisms Involved in the Beneficial Effects of Spironolactone after Myocardial Infarction

Acute Doxorubicin-Induced Cardiotoxicity is Associated with Matrix Metalloproteinase-2 Alterations in Rats

Aldosterone Blockade Reduces Mortality without Changing Cardiac Remodeling in Spontaneously Hypertensive Rats

Contact Info

Product

Resources

About