Algibacter mikhailovii sp. nov., a novel marine bacterium of the family Flavobacteriaceae, and emended description of the genus Algibacter

Abstract

Algibacter mikhailovii sp. nov., a novel marine bacterium of the family Flavobacteriaceae, and emended description of the genus Algibacter A novel marine bacterium, designated strain KMM 6171 T , was subjected to taxonomic analysis by using a polyphasic approach. Colonies were yellow-pigmented and cells were Gram-negative, heterotrophic rods displaying slow gliding motility. 16S rRNA gene sequence analysis indicated that strain KMM 6171 T was closely related to the genus Algibacter, a member of the family Flavobacteriaceae, with sequence similarity of 96.7-96.8 %. The predominant cellular fatty acids were iso-C15 : 1, iso-C15 : 0, anteiso-C15 : 0, C15 : 0, iso-C15 : 0 3-OH, iso-C17 : 0 3-OH and summed feature 3, comprising C16 : 1v7c and/or iso-C15 : 0 2-OH. The DNA G+C content was 35.1 mol%. On the basis of the phenotypic, genotypic, chemotaxonomic and phylogenetic data, strain KMM 6171 T represents a novel species of the genus Algibacter, for which the name Algibacter mikhailovii sp. nov. is proposed. The type strain is KMM 6171 T (5KCTC 12710 T 5LMG 23988 T ). An emended description of the genus Algibacter based on the new data is also given.The genus Algibacter, a member of the family Flavobacteriaceae (Bernardet et al., 2002), was erected to accommodate Gram-negative, facultatively anaerobic, gliding, orange-pigmented and agarolytic marine bacteria isolated from the surfaces of green algae (Nedashkovskaya et al. 2004).In the present work, we report the isolation and identification of a novel Gram-negative, gliding, yellow-pigmented marine bacterium, designated strain KMM 6171 T . As a result of a polyphasic study, including phylogenetic, genotypic, chemotaxonomic and phenotypic methods, the isolate was identified as a novel member of the genus Algibacter.The agarolytic strain KMM 6171 T was isolated from a sea urchin, Strongylocentrotus intermedius, collected in Troitsa Bay, Gulf of Peter the Great, the East Sea (also known as the Sea of Japan). For strain isolation, 0.1 ml homogenates of the sea urchin tissues were transferred onto plates of marine agar 2216 (Difco). After primary isolation and purification, strains were cultivated at 28 u C on the same medium and stored at -80 u C in marine broth (Difco) supplemented with 20 % (v/v) glycerol.DNA extraction, PCR and 16S rRNA gene sequencing were carried out as described previously (Vancanneyt et al., 2006). Sequence data obtained were aligned with those of representative members of the family Flavobacteriaceae retrieved from GenBank, and construction of a neighbourjoining (Saitou & Nei, 1987) phylogenetic tree and bootstrap analysis were carried out as described previously (Cho et al., 2006). In addition, trees were constructed on the basis of maximum-parsimony (Kluge & Farris, 1969) and maximum-likelihood (Felsenstein, 1993) algorithms.Phylogenetic analysis of the almost-complete 16S rRNA gene sequence of strain KMM 6171 T (1473 nt) revealed that the strain was affiliated with the family Flavobacteriaceae and formed a distinct lineage within the genus Alg...

Isolation and Complete Genome Sequence of Algibacter alginolytica sp. nov., a Novel Seaweed-Degrading Bacteroidetes Bacterium with Diverse Putative Polysaccharide Utilization Loci

“…A comparison of the physiological and biochemical characteristics of strain HZ22 and reference strains is shown in Table 1. For instance, strain HZ22 can grow at 4 to 55°C and 0 to 8% NaCl, higher temperature and NaCl concentration ranges than those of reference strains (35°C and 6% NaCl are the thresholds for most species of the genus Algibacter), indicating that strain HZ22 has an advantage in severe environments (20,25,26,28). The hydrolysis of agar, esculin, starch, and L-tyrosine for strain HZ22 T contrasts with that of reference strains.…”

“…The bacterium was tested for the Gram reaction by using the Gram-staining method (45). Cell morphology was examined by optical microscopy (BX40 microscope; Olympus) and transmission electron microscopy (JEM-1230 microscope; JEOL) using exponentially growing cells that were incubated on MA for 24 h. Growth at various NaCl concentrations (0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 6, 8, 10, and 12% [wt/vol]), a range of pHs (pH 5.0 to 10.5, with an interval of 0.5), and a range of temperatures (4,10,15,20,25,28,30,35,37,40,42, and 45°C) was carried out according to a protocol described previously (46). Catalase and oxidase were detected as described previously (45).…”

“…At the time of writing, according to the List of Prokaryotic Names with Standing in Nomenclature (21), there were 12 species in the genus Algibacter. Algibacter species have been isolated from seawater (22), green algae (20), intertidal sediment (23), marine sediment (24), sea urchins (25), and brown alga reservoirs (26)(27)(28). Most Algibacter species are isolated from or near the habitat of algae, indicating a preference for the complex polysaccharides of algae.…”

Pontimicrobium aquaticum gen. nov., sp. nov., a bacterium in the family Flavobacteriaceae isolated from seawater

“…The family Flavobacteriaceae [1] consists of many marine species such as Algibacter [2], Flavobacterium [3], Gaetbulibacter [4], Lacinutrix [5], Maribacter [6], Oceanihabitans [7] and Salegentibacter [8]. The family is found in various habitats, such as red algae, marine sediment, tidal flat sediment, antarctic marine, arctic ocean and ice lake.…”

Jejuia pallidilutea gen. nov., sp. nov., a new member of the family Flavobacteriaceae isolated from seawater

“…The family contains a well-defined 'marine clade', members of which are widespread in marine habitats where they contribute to the mineralization of primary-produced organic matter. They have been isolated from seawater and ice, quartz or basalt stones, marine sediment and algae (Bowman & Nichols, 2005; Einen & Øvreås, 2006; Jung et al., 2005;Kwon et al, 2006a, b;Lee, 2007;Nedashkovskaya et al, 2006Nedashkovskaya et al, , 2007Yang & Cho, 2008). In the course of investigations of bacterial communities in seawater, a novel Gram-stain-negative bacterium that produced pale orange pigments was isolated and investigated using a polyphasic approach.…”