Alginate as immobilization matrix and stabilizing agent in a two-phase liquid system: Application in lipase-catalysed reactions

Synthesis of geranyl acetate by lipase entrap‐immobilized in cellulose acetate‐TiO2 gel fiber

“…Leakage of lipase from acrylic resin (Novozyme) and CA-TiO 2 gel fiber (fiber-immobilized lipase) into an aqueous solution was examined with reference to the studies of Hertzberg et al (13). Novozyme (20 mg), fiber-immobilized lipase (0.8 g), or CATiO 2 gel fiber (0.8 g, no enzymes were contained) was added to 10 mL of water.…”

Effects of preparative parameters on the properties of chitosan hydrogel beads containing Candida rugosa lipase

“…The release of lipase into a pH 7.2, 0.05 m Tris HCl buffer was performed over a 36 h period to evaluate the loss of lipase from the beads after the manufacturing process was complete. Release studies were conducted in Tris HCl buffer because this buffer was used during the manufacturing process and also because lipase was found to be stable in this buffer [29].…”

Immobilization of Candida rugosa lipase in lyotropic liquid crystals and some properties of the immobilized enzyme

“…Due to inhibitory effects of organic solvents, immobilization of the biocatalysts is often recommended. Besides entrapment in hydrophilic gels (Hertzberg et aL, 1992), deposition on solid supports (Montero et al, 1993) and solubilization in reversed micelles (Yang and Russell, 1995), immobilization in lyotropic liquid crystals is a fasci-nating new method (Frense et aL, 1992;Razumas et al, 1994;Hochkoeppler et aL, 1995).…”