Alginate-based hydrogels show the same complex mechanical behavior as brain tissue

Distler, Thomas; Schaller, E.; Steinmann, Paul; Boccaccını, Aldo R.; Budday, Silvia

doi:10.1016/j.jmbbm.2020.103979

Cited by 47 publications

(29 citation statements)

References 75 publications

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“…Paper specimen holder before mounting the samples by using instant glue (Cyanacrylat, Henkel AG & Co. KGaA, Germany), as described in ref. 38. Before starting the testing protocol, an axial force of 0.03 N was applied to ensure contact between the geometry and the specimen.…”

Section: Biomaterials Sciencementioning

confidence: 99%

“…38 In contrast to fibrin-based hydrogels, 50 the matrix material showed a stiffer behavior for compression than for tension, mimicking the asymmetry found in native brain tissue. 38,51 Yet, matrices different from pristine alginate are required to allow for matrix remodeling, cell proliferation, and hydrogel degradation, to ensure successful TE approaches. 43 To overcome the limited cell-material interaction and degradation behavior of pristine alginate, advanced alginatebased hydrogels have been introduced, 19,61,68 which include derivatives with increased cell-material interaction through specific integrin binding motifs 28,[69][70][71][72] and proteins, 31,73,74 combined with a tailored degradation behavior through oxidation of the alginate.…”

Section: Introductionmentioning

confidence: 97%

“…[31][32][33][34][35] Previous studies have shown that neural progenitor or pre-osteoblast cells are more likely to adhere, differentiate, and proliferate when the artificial ECM has similar properties to the native ECM. 36,37 Importantly, the mechanical characteristics of biomaterial hydrogels can be tuned by various means, such as composition, 32,35,[38][39][40][41][42] chemical functionalities, 43 microstructure, 44 and cross-linking mechanisms. [45][46][47] Research on the mechanical properties of materials for TE, therefore, has been frequently focused on a single stiffness value and how it affects cell adhesion, proliferation, and differentiation.…”

Section: Introductionmentioning

confidence: 99%

“…64 Mechanical tissue-and organ-substitutes, to be used as, e.g., soft tissue phantoms or surgical models, have been engineered using various hydrogels. 38,50,[65][66][67] We previously found that alginate-based gels without embedded cells can imitate mechanical characteristics of brain tissue. 38 In contrast to fibrin-based hydrogels, 50 the matrix material showed a stiffer behavior for compression than for tension, mimicking the asymmetry found in native brain tissue.…”

Section: Introductionmentioning

confidence: 99%

“…38,50,[65][66][67] We previously found that alginate-based gels without embedded cells can imitate mechanical characteristics of brain tissue. 38 In contrast to fibrin-based hydrogels, 50 the matrix material showed a stiffer behavior for compression than for tension, mimicking the asymmetry found in native brain tissue. 38,51 Yet, matrices different from pristine alginate are required to allow for matrix remodeling, cell proliferation, and hydrogel degradation, to ensure successful TE approaches.…”

Section: Introductionmentioning

confidence: 99%

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Mechanical properties of cell- and microgel bead-laden oxidized alginate-gelatin hydrogels

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Section: Biomaterials Sciencementioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Breast Tumor Cell Survival and Morphology in a Brain‐like Extracellular Matrix Depends on Matrix Composition and Mechanical Properties

Türker,

Andrade Mier,

Faber

et al. 2024

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Triple‐negative breast cancer (TNBC) is the most invasive type of breast cancer with high risk of brain metastasis. To better understand interactions between breast tumors with the brain extracellular matrix (ECM), a 3D cell culture model is implemented using a thiolated hyaluronic acid (HA‐SH) based hydrogel. The latter is used as HA represents a major component of brain ECM. Melt‐electrowritten (MEW) scaffolds of box‐ and triangular‐shaped polycaprolactone (PCL) micro‐fibers for hydrogel reinforcement are utilized. Two different molecular weight HA‐SH materials (230 and 420 kDa) are used with elastic moduli of 148 ± 34 Pa (soft) and 1274 ± 440 Pa (stiff). Both hydrogels demonstrate similar porosities. The different molecular weight of HA‐SH, however, significantly changes mechanical properties, e.g., stiffness, nonlinearity, and hysteresis. The breast tumor cell line MDA‐MB‐231 forms mainly multicellular aggregates in both HA‐SH hydrogels but sustains high viability (75%). Supplementation of HA‐SH hydrogels with ECM components does not affect gene expression but improves cell viability and impacts cellular distribution and morphology. The presence of other brain cell types further support numerous cell–cell interactions with tumor cells. In summary, the present 3D cell culture model represents a novel tool establishing a disease cell culture model in a systematic way.

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Assembled Embedded 3D Hydrogel System for Asynchronous Drug Delivery to Inhibit Postoperative Recurrence of Malignant Glioma and Promote Neurological Recovery

Hu,

Zhou,

Wang

et al. 2024

Adv Funct Materials

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Surgical resection of glioblastoma multiforme (GBM) often results in tumor recurrence and mild neurologic deficits. Here, a 3D asynchronous drug delivery system is innovatively developed to address the dual challenges of GBM recurrence and postoperative neurological deficit. Based on transcriptome analysis of tumor cells and tumor microenvironment (TME) cells between primary and recurrent mouse GBM tissues, a novel dual‐targeting approach is developed to combine mTOR pathway inhibition with microglia/macrophage repolarization. Then, in situ injectable methacrylated gelatin (GelMA) is constructed to perfectly fit into the tumor resection cavity and achieve direct delivery of dual‐targeted drugs, exhibiting outstanding postoperative GBM inhibitory effects in vivo. At the same time, neurotrophic factor‐saturated 3D‐printed GelMA patches are used to construct a 3D asynchronous drug delivery system, allowing gradual penetration of the neurotrophic factors into the underlying hydrogel to promote axonal sprouting after GBM suppression. Notably, this 3D asynchronous drug delivery system promotes neurological recovery without weakening the efficacy of inhibiting tumor recurrence. Therefore, this study not only proposes a new dual‐targeted GBM treatment strategy but also pioneers the construction of a 3D asynchronous drug delivery system for the comprehensive treatment of GBM. This study is expected to improve the poor prognosis of patients with GBM.

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Alginate-based hydrogels show the same complex mechanical behavior as brain tissue

Cited by 47 publications

References 75 publications

Mechanical properties of cell- and microgel bead-laden oxidized alginate-gelatin hydrogels

Mechanical properties of cell- and microgel bead-laden oxidized alginate-gelatin hydrogels

Breast Tumor Cell Survival and Morphology in a Brain‐like Extracellular Matrix Depends on Matrix Composition and Mechanical Properties

Assembled Embedded 3D Hydrogel System for Asynchronous Drug Delivery to Inhibit Postoperative Recurrence of Malignant Glioma and Promote Neurological Recovery

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