Alginate-based tissue-specific bioinks for multi-material 3D-bioprinting of pancreatic islets and blood vessels: A step towards vascularized pancreas grafts

Idaszek, Joanna; Volpi, Marina; Paradiso, Alessia; Quoc, Martyna Nguyen; Górecka, Żaneta; Klak, Marta; Tymicki, Grzegorz; Berman, Andrzej; Wierzbicki, Mateusz; Jaworski, Sławomir; Costantini, Marco; Kępczyńska, Agnieszka; Chwalibóg, Ewa Sawosz; Wszoła, Michał; Święszkowski, Wojciech

doi:10.1016/j.bprint.2021.e00163

Cited by 36 publications

(26 citation statements)

References 73 publications

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“…The induction of T1D with the occurrence of an immune reaction and the analysis of antibodies is essential for the research model to observe the course of T1D. On the other hand, in order to evaluate potential diabetes treatment methods, e.g., with stem cell therapy [ 59 ], transplantation of pancreatic islets [ 60 ], or a bioprinting model with beta-cells or pancreatic islets [ 61 ], the result of diabetes induction remains critical. Again, the key marker is the C-peptide concentration analysis.…”

Section: Discussionmentioning

confidence: 99%

Streptozotocin-Induced Diabetes in a Mouse Model (BALB/c) Is Not an Effective Model for Research on Transplantation Procedures in the Treatment of Type 1 Diabetes

Wszoła¹,

Klak²,

Kosowska

et al. 2021

Biomedicines

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Type 1 diabetes (T1D) is characterized by the destruction of over 90% of the β-cells. C-peptide is a parameter for evaluating T1D. Streptozotocin (STZ) is a standard method of inducing diabetes in animals. Eight protocols describe the administration of STZ in mice; C-peptide levels are not taken into account. The aim of the study is to determine whether the STZ protocol for the induction of beta-cell mass destruction allows for the development of a stable in vivo mouse model for research into new transplant procedures in the treatment of type 1 diabetes. Materials and methods: Forty BALB/c mice were used. The animals were divided into nine groups according to the STZ dose and a control group. The STZ doses were between 140 and 400 mg/kg of body weight. C-peptide was taken before and 2, 7, 9, 12, 14, and 21 days after STZ. Immunohistochemistry was performed. The area of the islet and insulin-/glucagon-expressing tissues was calculated. Results: Mice who received 140, 160, 2 × 100, 200, and 250 mg of STZ did not show changes in mean fasting C-peptide in comparison to the control group and to day 0. All animals with doses of 300 and 400 mg of STZ died during the experiment. The area of the islets did not show any differences between the control and STZ-treated mice in groups below 300 mg. The reduction of insulin-positive areas in STZ mice did not exceed 50%. Conclusions: Streptozotocin is not an appropriate method of inducing a diabetes model for further research on transplantation treatments of type 1 diabetes, having caused the destruction of more than 90% of the β-cell mass in BALB/c mice.

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Section: Discussionmentioning

confidence: 99%

Streptozotocin-Induced Diabetes in a Mouse Model (BALB/c) Is Not an Effective Model for Research on Transplantation Procedures in the Treatment of Type 1 Diabetes

Wszoła¹,

Klak²,

Kosowska

et al. 2021

Biomedicines

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“…This strategy is often used in combination with coaxial nozzles, enabling the simultaneous delivery of both cross-linking solutions and a cell-laden hydrogel bioink through the outer and inner nozzle, respectively. 146 − 149 As a result of such on-process cross-linking, a rigid and stable cell-laden hydrogel filament is formed, thus preventing fibers from spreading or collapsing during the deposition process. Hence, the fabrication of high-resolution 3D constructs can be successfully performed.…”

Section: Hydrogel-based Fiber Biofabrication Methodsmentioning

confidence: 99%

Hydrogel-Based Fiber Biofabrication Techniques for Skeletal Muscle Tissue Engineering

Volpi

Paradiso

Costantini

et al. 2022

ACS Biomater. Sci. Eng.

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The functional capabilities of skeletal muscle are strongly correlated with its well-arranged microstructure, consisting of parallelly aligned myotubes. In case of extensive muscle loss, the endogenous regenerative capacity is hindered by scar tissue formation, which compromises the native muscle structure, ultimately leading to severe functional impairment. To address such an issue, skeletal muscle tissue engineering (SMTE) attempts to fabricate in vitro bioartificial muscle tissue constructs to assist and accelerate the regeneration process. Due to its dynamic nature, SMTE strategies must employ suitable biomaterials (combined with muscle progenitors) and proper 3D architectures. In light of this, 3D fiber-based strategies are gaining increasing interest for the generation of hydrogel microfibers as advanced skeletal muscle constructs. Indeed, hydrogels possess exceptional biomimetic properties, while the fiber-shaped morphology allows for the creation of geometrical cues to guarantee proper myoblast alignment. In this review, we summarize commonly used hydrogels in SMTE and their main properties, and we discuss the first efforts to engineer hydrogels to guide myoblast anisotropic orientation. Then, we focus on presenting the main hydrogel fiber-based techniques for SMTE, including molding, electrospinning, 3D bioprinting, extrusion, and microfluidic spinning. Furthermore, we describe the effect of external stimulation (i.e., mechanical and electrical) on such constructs and the application of hydrogel fiber-based methods on recapitulating complex skeletal muscle tissue interfaces. Finally, we discuss the future developments in the application of hydrogel microfibers for SMTE.

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“…Despite having varying rheological characteristics, the prepared bioinks were 3D printed with good shape fidelity utilizing a multichannel microfluidic platform and a co-axial needle system. To test the bioactivity, high viability 3D-bioprinted bioinks were loaded with swine pancreatic islets and a combination of vessel-forming cells (HUVEC and HMSC) 157 (Fig. 12b and c).…”

Section: Applications Of 3d Bioprintingmentioning

confidence: 99%

A focused review on three-dimensional bioprinting technology for artificial organ fabrication

et al. 2022

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Alginate-based tissue-specific bioinks for multi-material 3D-bioprinting of pancreatic islets and blood vessels: A step towards vascularized pancreas grafts

Cited by 36 publications

References 73 publications

Streptozotocin-Induced Diabetes in a Mouse Model (BALB/c) Is Not an Effective Model for Research on Transplantation Procedures in the Treatment of Type 1 Diabetes

Streptozotocin-Induced Diabetes in a Mouse Model (BALB/c) Is Not an Effective Model for Research on Transplantation Procedures in the Treatment of Type 1 Diabetes

Hydrogel-Based Fiber Biofabrication Techniques for Skeletal Muscle Tissue Engineering

A focused review on three-dimensional bioprinting technology for artificial organ fabrication

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