Alginate modifying enzymes: An updated comprehensive review of the mannuronan C5-epimerases

Cao, Shengsheng; Li, Li; Zhu, Benwei; Yao, Zhong

doi:10.1016/j.algal.2022.102952

Cited by 4 publications

(2 citation statements)

References 100 publications

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“…To convert M components into G components of alginate, these microorganisms utilize two types of MC5Es, AlgG and AlgE. A variety of MC5Es reacts to different arrays of alginate, introducing G components [49,50], and thereby producing alginate composed of diverse monomer distribution blocks. Additionally, AvAlgE7, AcAlgE2, and AcAlgE3, have a potential for degrading alginate through lyase activity among AlgE-type MC5Es from A. vinelandii and A. chroococcum [36,37].…”

Section: Discussionmentioning

confidence: 99%

Structural basis for the minimal bifunctional alginate epimerase AlgE3 from Azotobacter chroococcum

Fujiwara,

Mano,

Nango

2024

FEBS Letters

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Among the epimerases specific to alginate, some of them in Azotobacter genera convert β‐d‐mannuronic acid to α‐l‐guluronic acid but also have lyase activity to degrade alginate. The remarkable characteristics of these epimerases make it a promising enzyme for tailoring alginates to meet specific demands. Here, we determined the structure of the bifunctional mannuronan C‐5 epimerase AlgE3 from Azotobacter chroococcum (AcAlgE3) in complex with several mannuronic acid oligomers as well as in apo form, which allowed us to elucidate the binding manner of each mannuronic acid oligomer, and the structural plasticity, which is dependent on calcium ions. Moreover, a comprehensive analysis of the lyase activity profiles of AcAlgE3 combined with structural characteristics explained the preference for different chain length oligomers.

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Section: Discussionmentioning

confidence: 99%

Structural basis for the minimal bifunctional alginate epimerase AlgE3 from Azotobacter chroococcum

Fujiwara,

Mano,

Nango

2024

FEBS Letters

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“…In the case of this natural polysaccharide, instant gelation can easily be achieved through contact with multivalent cations, the most used being Ca 2+ [23,24]. Owing to the natural characteristic of alginate (a copolymer of guluronic acid and mannuronic acid), and easy conditions of use and processing, Alg-based biomaterials have been intensively studied for modern biomedicine, especially during the last decades [25,26].…”

Section: Introductionmentioning

confidence: 99%

Infection-Free and Enhanced Wound Healing Potential of Alginate Gels Incorporating Silver and Tannylated Calcium Peroxide Nanoparticles

Bîrcă,

Gherasim,

Niculescu

et al. 2024

IJMS

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The treatment of chronic wounds involves precise requirements and complex challenges, as the healing process cannot go beyond the inflammatory phase, therefore increasing the healing time and implying a higher risk of opportunistic infection. Following a better understanding of the healing process, oxygen supply has been validated as a therapeutic approach to improve and speed up wound healing. Moreover, the local implications of antimicrobial agents (such as silver-based nano-compounds) significantly support the normal healing process, by combating bacterial contamination and colonization. In this study, silver (S) and tannylated calcium peroxide (CaO2@TA) nanoparticles were obtained by adapted microfluidic and precipitation synthesis methods, respectively. After complementary physicochemical evaluation, both types of nanoparticles were loaded in (Alg) alginate-based gels that were further evaluated as possible dressings for wound healing. The obtained composites showed a porous structure and uniform distribution of nanoparticles through the polymeric matrix (evidenced by spectrophotometric analysis and electron microscopy studies), together with a good swelling capacity. The as-proposed gel dressings exhibited a constant and suitable concentration of released oxygen, as shown for up to eight hours (UV–Vis investigation). The biofilm modulation data indicated a synergistic antimicrobial effect between silver and tannylated calcium peroxide nanoparticles, with a prominent inhibitory action against the Gram-positive bacterial biofilm after 48 h. Beneficial effects in the human keratinocytes cultured in contact with the obtained materials were demonstrated by the performed tests, such as MTT, LDH, and NO.

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Adsorption of sodium alginate on calcium carbonate microparticles: Effect of molar mass and composition

Da Costa,

Ghobril,

Perrin

et al. 2024

Colloids and Surfaces A: Physicochemical and Engineering Aspect

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Alginate modifying enzymes: An updated comprehensive review of the mannuronan C5-epimerases

Cited by 4 publications

References 100 publications

Structural basis for the minimal bifunctional alginate epimerase AlgE3 from Azotobacter chroococcum

Structural basis for the minimal bifunctional alginate epimerase AlgE3 from Azotobacter chroococcum

Infection-Free and Enhanced Wound Healing Potential of Alginate Gels Incorporating Silver and Tannylated Calcium Peroxide Nanoparticles

Adsorption of sodium alginate on calcium carbonate microparticles: Effect of molar mass and composition

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