Algoriphagus roseus sp. nov., isolated from a hexachlorocyclohexane-contaminated dumpsite

Abstract

A Gram-staining negative, reddish-pink, non-motile, rod-shaped bacterial strain designated W29 T , was isolated from a hexachlorocyclohexane-contaminated dumpsite located in the northern part of India at Ummari Village, Lucknow. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain W29 T formed a lineage within the genus Algoriphagus and exhibited highest sequence similarity to Algoriphagus trabzonensis MS7 T (98.8 %), followed by Algoriphagus alkaliphilus AC-74 T (97.1 %). The 16S rRNA gene sequence similarity between strain W29 T and other species of the genus Algoriphagus ranged from 93.3-98.8 %. The DNA-DNA relatedness between strain W29 T and A. trabzonensis MS7 T was 47 % and with other related strains was found to be less than 45 %, confirming strain W29 T represents a novel species. The DNA G+C content of strain W29 T was 46.2 mol%. Strain W29 T was oxidase-and catalase-positive. The major fatty acids (>10 %) of strain W29 T were iso-C 15 : 0 , summed feature 9 (comprising 10-methyl C 16 : 0 and/or iso-C 17 : 1 !9c) and summed feature 3 (comprising C 16 : 1 !6c and/or C 16 : 1 !7c). The respiratory quinone was MK-7. The polar lipid profile consisted of phosphatidylethanolamine, diphosphatidylglycerol, two unidentified aminolipids, an unidentified aminophospholipid, an unidentified phospholipid and unidentified lipids. On the basis of the results obtained from DNA-DNA hybridization, and biochemical and physiological tests in this study, strain W29 T represents a novel species of the genus Algoriphagus for which the name Algoriphagus roseus sp. nov. is proposed. The type strain is W29 T (=KCTC 42940 T =MCC 2876 T =DSM 100160 T ).