Alhagi kirghisorum: Technological Aspects of its Thick Extract for the Pharmaceutical Application

Kukhtenko, H. P.

doi:10.15414/ainhlq.2022.0009

Cited by 2 publications

(2 citation statements)

References 9 publications

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“…Extracts from Rh. tomentosum shoots were obtained using a modified method that combined the effects of ultrasound and temperature to maximize the extraction of biologically active substances from the raw material [28,[57][58][59]. Extracts were prepared using different concentrations of hydroalcoholic mixture and purified water to determine the effect of ethanol concentration on the extraction of flavonoids in the form of aglycones and glycosides since glycosidic and aglyconic forms of flavonoids have different solubility [27,60,61].…”

Section: Extracts Preparationmentioning

confidence: 99%

Spectrophotometric and Chromatographic Assessment of Total Polyphenol and Flavonoid Content in Rhododendron tomentosum Extracts and Their Antioxidant and Antimicrobial Activity

Kukhtenko,

Bevz,

Konechnyi

et al. 2024

Molecules

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In the literature, the chemical composition of Rhododendron tomentosum is mainly represented by the study of isoprenoid compounds of essential oil. In contrast, the study of the content of flavonoids will contribute to the expansion of pharmacological action and the use of the medicinal plant for medical purposes. The paper deals with the technology of extracts from Rh. tomentosum shoots using ethanol of various concentrations and purified water as an extractant. Extracts from Rh. tomentosum were obtained by a modified method that combined the effects of ultrasound and temperature to maximize the extraction of biologically active substances from the raw material. Using the method of high-performance thin-layer chromatography in a system with solvents ethyl acetate/formic acid/water (15:1:1), the following substances have been separated and identified in all the extracts obtained: rutin, hyperoside, quercetin, and chlorogenic acid. The total polyphenol content (TPC) and total flavonoid content (TFC) were estimated using spectrophotometric methods involving the Folin-Ciocalteu (F-C) reagent and the complexation reaction with aluminum chloride, respectively. A correlation analysis was conducted between antioxidant activity and the polyphenolic substance content. Following the DPPH assay, regression analysis shows that phenolic compounds contribute to about 80% (r2 = 0.8028, p < 0.05) of radical scavenging properties in the extract of Rh. tomentosum. The extract of Rh. tomentosum obtained by ethanol 30% inhibits the growth of test cultures of microorganisms in 1:1 and 1:2 dilutions of the clinical strains #211 Staphylococcus aureus and #222 Enterococcus spp. and the reference strain Pseudomonas aeruginosa ATCC 10145.

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Section: Extracts Preparationmentioning

confidence: 99%

Spectrophotometric and Chromatographic Assessment of Total Polyphenol and Flavonoid Content in Rhododendron tomentosum Extracts and Their Antioxidant and Antimicrobial Activity

Kukhtenko,

Bevz,

Konechnyi

et al. 2024

Molecules

Self Cite

View full text Add to dashboard Cite

show abstract

“…Extracts from Rhododendron tomentosum shoots were obtained using a modified method that combined the effects of ultrasound and temperature to maximize the extraction of biologically active substances from the raw material [11,[39][40][41]. Extracts were prepared using different concentrations of hydroalcoholic mixture and purified water to determine the effect of ethanol concentration on the extraction of flavonoids in the form of aglycones and glycosides since glycosidic and aglyconic forms of flavonoids have different solubility [10,42,43].…”

Section: Extracts Preparationmentioning

confidence: 99%

Spectrophotometric and Chromatographic Assessment of Total Flavonoids Content in Rhodendron Tomentosum Extracts

Kukhtenko,

Bevz,

Kukhtenko

et al. 2023

Preprint

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In the literature, the chemical composition of Rhododendron tomentosum is mainly represented by the study of isoprenoid compounds of essential oil. In contrast, the study of the content of flavonoids will contribute to the expansion of the pharmacological action and the use of the medicinal plant for medical purposes. The paper deals with the technology of extracts from Rhododendron tomentosum shoots using ethanol of various concentrations and purified water as an extractant. Extracts from Rhododendron tomentosum were obtained by a modified method that combined the effects of ultrasound and temperature to maximize the extraction of biologically active substances from the raw material. By the method of high-performance thin-layer chromatography in the system of solvents ethyl acetate: formic acid: water (15:1:1) the following substances have been separated and identified: rutin, hyperoside, quercetin, and chlorogenic acid in all the extracts obtained. To quantify the total flavonoids content, a method of spectrophotometric determination based on the reaction of complexation with aluminum chloride has been developed. It has been proved that the course of the complexation reaction and, accordingly, the quantification of the flavonoid structure substances by the spectrophotometric method is influenced by some factors, including the environment, pH, the structure of the complexes, etc. Regardless of the chosen method, for the further production of Rhododendron tomentosum extracts with a higher content of flavonoid structure substance, it is advisable to use 60%, 70%, and 80% ethyl alcohol as extractant.

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Alhagi kirghisorum: Technological Aspects of its Thick Extract for the Pharmaceutical Application

Cited by 2 publications

References 9 publications

Spectrophotometric and Chromatographic Assessment of Total Polyphenol and Flavonoid Content in Rhododendron tomentosum Extracts and Their Antioxidant and Antimicrobial Activity

Spectrophotometric and Chromatographic Assessment of Total Polyphenol and Flavonoid Content in Rhododendron tomentosum Extracts and Their Antioxidant and Antimicrobial Activity

Spectrophotometric and Chromatographic Assessment of Total Flavonoids Content in Rhodendron Tomentosum Extracts

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