Alkaline phosphatase from rat liver and kidney is differentially modulated

Martins, Maria João; Negrão, Rita; Hipólito-Reis, C.

doi:10.1016/s0009-9120(01)00255-7

Cited by 43 publications

(37 citation statements)

References 34 publications

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“…In vitro data from our group regarding ALP activity modulation will be presented shortly in the discussion of this article, since some of our previous results will be useful for comparison with the present data (13,14,(17)(18)(19).…”

Section: Introductionmentioning

confidence: 92%

“…On the day of ALP quantification, tissue samples diluted further in homogenization solution (to 1 g tissue + 12 mL homogenization solution) were homogenized and kept on ice. ALP activity assays were carried out in triplicate, as previously described (13,15). The reac- 3) was performed with total RNA prepared from rat heart homogenate (left ventricle).…”

Section: Alkaline Phosphatase Activitymentioning

confidence: 99%

“…Only humans and great apes have P-ALP; all other mammals have instead Int-ALP (2,12). Tissue differential processing of each of the ALP gene products, for example through differential glycosylation, gives rise to tissue-specific isoforms (2,13). Depending on the physiological/pathological conditions ALP can be released into intestinal lumen, plasma or bile in various isoforms: with or without its anchor and in the former case associated or not with membrane fragments (2).…”

Section: Introductionmentioning

confidence: 99%

“…Depending on the physiological/pathological conditions ALP can be released into intestinal lumen, plasma or bile in various isoforms: with or without its anchor and in the former case associated or not with membrane fragments (2). ALP isoenzymes (and isoforms) can be distinguished by their heat stability, optimum pH, substrate affinity or effect of several specific inhibitors or activators (2,(13)(14)(15)(16)(17)(18)(19).…”

Section: Introductionmentioning

confidence: 99%

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Characterization of rat heart alkaline phosphatase isoenzymes and modulation of activity

Mota

Silva

Neves

et al. 2008

Braz J Med Biol Res

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Alkaline phosphatase (ALP) is important in calcification and its expression seems to be associated with the inflammatory process. We investigated the in vitro acute effects of compounds used for the prevention or treatment of cardiovascular diseases on total ALP activity from male Wistar rat heart homogenate. ALP activity was determined by quantifying, at 410 nm, the pnitrophenol released from p-nitrophenylphosphate (substrate in Tris buffer, pH 10.4). Using specific inhibitors of ALP activity and the reverse transcription-polymerase chain reaction, we showed that the rat heart had high ALP activity (31.73 ± 3.43 nmol pnitrophenol·mg protein -1 ·min -1 ): mainly tissue-nonspecific ALP but also tissue-specific intestinal ALP type II. Both ALP isoenzymes presented myocardial localization (striated pattern) by immunofluorescence. ALP was inhibited a) strongly by 0.5 mM levamisole, 2 mM theophylline and 2 mM aspirin (91, 77 and 84%, respectively) and b) less strongly by 2 mM Lphenylalanine, 100 µL polyphenol-rich beverages and 0.5 mM progesterone (24, 21 to 29 and 11%, respectively). ß-estradiol and caffeine (0.5 and 2 mM) had no effect; 0.5 mM simvastatin and 2 mM atenolol activated ALP (32 and 36%, respectively). Propranolol (2 mM) tended to activate ALP activity and corticosterone activated (18%) and inhibited (13%) (0.5 and 2 mM, respectively). We report, for the first time, that the rat heart expresses intestinal ALP type II and has high total ALP activity. ALP activity was inhibited by compounds used in the prevention of cardiovascular pathology. ALP manipulation in vivo may constitute an additional target for intervention in cardiovascular diseases.Key words: Heart; Alkaline phosphatase; Polyphenol-rich beverages; Steroid hormones; Methylxanthines A preliminary report of this manuscript was presented at the "Experimental Biology" Congress, San Francisco, CA, USA, April 1-5, 2006 in abstract form: Mota A, Calhau C, Martel F, Martins MJ. "Modulation of rat heart alkaline phosphatase activity by drugs, hormones and nutrients". Faseb J 2006; 20: A897 (Abstract 588.12).

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Section: Introductionmentioning

confidence: 92%

Section: Alkaline Phosphatase Activitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Characterization of rat heart alkaline phosphatase isoenzymes and modulation of activity

Mota

Silva

Neves

et al. 2008

Braz J Med Biol Res

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“…The formation f yellowish product, p-nitrophenol was determined based on the absorbance at 415nm using spectrophotometer with slight modification from the method described by Martins and co-workers (2001). 9 The amount of product was calculated based on the standard calibration curve of p-nitrophenol. Statistic Analysis: All data were reported as mean ± standard deviation (SD).…”

Section: Introductionmentioning

confidence: 99%

Averrhoa Carambola (Star Fruit) Induces Hepatic Alkaline Phosphatase Activity in Rats

Chin¹,

Teh²,

Khoo³

et al. 2011

Int J of Biomed & Adv Res

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Effects of therapeutic and toxic doses of levamisole on thyroid hormones and some biochemical parameters in sheep

Ateşşahín

Karahan

Pirinçci

2004

Cell Biochemistry & Function

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This study was carried out to establish the effects of therapeutic and toxic doses of levamisole on thyroid hormone levels and some biochemical parameters in sheep. Twelve Akkaraman ewes were used. Levamisole was given orally at doses of 7.5 mg kg(-1) (group 1) and 40 mg kg(-1) (group 2) to the animals. Blood samples were taken from the jugular vein at 2, 4, 8, 24, 48, 96 and 144 h after the administrations. Serum thyroid hormones and some biochemical parameters were determined on these samples. When compared with the control levels, no significant changes were observed in triiodothyronine (T3) and thyroxin (T4) levels in group 1. Although levamisole was found to increase the levels of total T3, it decreased the levels of total T4 in group 2. On the other hand, free T3 and free T4 levels were not changed in either group. While serum alkaline phosphatase (ALP) activities were decreased, aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH) and creatinine kinase (CK) activities were increased significantly by levamisole. However, it increased the serum albumin and cholesterol levels, but decreased the inorganic phosphate levels in groups 1 and 2. On the other hand, when compared with the control levels, no significant changes were detected in serum sodium, potassium and calcium levels. In conclusion, therapeutic and toxic doses of levamisole were determined to affect thyroid metabolism and some biochemical parameters in sheep.

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Alkaline phosphatase from rat liver and kidney is differentially modulated

Cited by 43 publications

References 34 publications

Characterization of rat heart alkaline phosphatase isoenzymes and modulation of activity

Characterization of rat heart alkaline phosphatase isoenzymes and modulation of activity

Averrhoa Carambola (Star Fruit) Induces Hepatic Alkaline Phosphatase Activity in Rats

Effects of therapeutic and toxic doses of levamisole on thyroid hormones and some biochemical parameters in sheep

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