Alkaline phosphatase in adaptation to low dietary phosphate intake.

Kempson, Stephen A.; Kim, J K; Northrup, Thomas E.; Knox, Franklyn G.; Douša, Thomas P.

doi:10.1152/ajpendo.1979.237.5.e465

Cited by 39 publications

(26 citation statements)

References 35 publications

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“…Although association between AlPase and Pi transport in kidney (10,14,31) and intestine (56) has been observed both in previous studies and in the present study, it does not constitute a direct evidence that these two BBM components are causally related. It is of interest to note, however, that in intestinal epithelium, which in many ways is similar to renal brush border, Moog and Glazier (56) showed that the Pi transport varied in parallel with AlPase activity and that agents inhibiting AlPase activity resulted in the inhibition of Pi transport (56).…”

Section: Discussioncontrasting

confidence: 93%

“…Enhanced Na+-dependent Pi uptake by isolated BBM vesicles from rats on low P diet (9,10) indicates that transport across BBM is an important step in the adaptive phenomenon at least in proximal tubule. The only early biochemical change in BBM that accompanies P deprivation known at the present time is an increase in renal cortical AlPase activity (13,14) located in the BBM fraction (10,14). Melani et al (13,49) have shown that the increase in AlPase activity is a result of increased synthesis of this renal cortical enzyme.…”

Section: Discussionmentioning

confidence: 86%

“…This suggests that an increase in AlPase occurring in the response to low P diet (10,13,14) (53) and, specifically, synthesis of PTH (54). According to a recent report (51), the well-known resistance to phosphaturic effects of PTH (55) AlPase in BBM vesicles prepared from rats on a low P diet.…”

Section: Discussionmentioning

confidence: 99%

“…In P-deprived microorganisms AlPase is induced (11,12); an increase in AlPase activity was also observed in kidneys of rats placed on a low P diet (10,13,14). This increase in renal AlPase in rats was specific compared with a number of other renal enzymes (10,13,14), and BBM activity of AlPase was positively correlated with the rate of Na+-dependent 32Pi uptake across renal BBM (10).…”

mentioning

confidence: 88%

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Renal Adaptation to a Low Phosphate Diet in Rats

Shah¹,

Kempson²,

Northrup³

et al. 1979

J. Clin. Invest.

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A B S T R A C T The major renal adaptive changes in response to selective dietary phosphate restriction are a marked reduction in urinary excretion of phosphate and an increased urinary excretion of calcium; at the cellular level, there is selective increase in renal cortical brush border membrane phosphate uptake and increase in specific activity of alkaline phosphatase. In the present study we examined whether these functional and biochemical adaptive changes could be blocked by drugs known to inhibit protein synthesis.Administration of actinomycin D or cycloheximide to rats switched from a diet with normal phosphate content (0.7%) to a diet with low (0.07%) phosphate content either completely (actinomycin D) or partially (cycloheximide) prevented the expected decrease in urinary excretion of phosphate and increase in the urinary excretion of calcium. The specific activity of alkaline phosphatase measured in crude membrane fraction (washed 100,000 g pellet) from renal cortical homogenate in animals fed a low phosphate diet and treated with actinomycin D or with cycloheximide was significantly lower than in control animals also on a low phosphate diet receiving placebo; but there were no differences between treated and untreated animals in the activities of two other brush border enzymes, y-glutamyltransferase and leucine aminopeptidase. Actinomycin D administered to rats maintained on a normal phosphate diet throughout the course of the experiment caused an increase in the urinary excretion of phosphate on the last (6th) day of the experiment but did not change urinary excretion of calcium. In acute clearance experiments, infusion of actinomycin D to rats adapted to a low phosphate diet did not increase fractional excretion of phosphate.In separate experiments, using the same dietary protocol as above, brush border membrane fraction (vesicles) was prepared from renal cortex of rats sacrificed at the end of the experiment. In this preparation Na+-dependent 32Pi and D-[3H]glucose uptake and activities of brush border enzymes membrane were determined. Brush border membrane vesicles prepared from rats fed a low phosphate diet showed significantly higher Na+-dependent 32Pi uptake compared with rats fed a normal phosphate diet. This increase in -'Pi uptake was completely prevented when rats on a low phosphate diet were simultaneously treated with actinomycin D. These differences were specific for 32Pi transport as no differences were observed in D_[3H]glucose uptake among the three groups. There was a positive correlation (r = 0.82, P < 0.01) between 32Pi uptake and specific activity of alkaline phosphatase measured in aliquots ofthe same brush border membranes, whereas no such correlation was observed with two other brush border membrane enzymes y-glutamyltransferase and leucine aminopeptidase.These observations show that actinomycin D prevents both the functional and cellular renal adaptive changes induced by a low phosphate diet. Taken together, these observations suggest that renal adaptation to a low phosphate ...

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Section: Discussioncontrasting

confidence: 93%

Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 88%

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Renal Adaptation to a Low Phosphate Diet in Rats

Shah¹,

Kempson²,

Northrup³

et al. 1979

J. Clin. Invest.

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“…et la phosphatase alcaline (E.C.3.1.3.1.) sont susceptibles d'intervenir dans l'hydrolyse des phytates (McCuaig et Motzok, 1972 ;Davies, Ritcey et Motzok, 1970 ; Flett, 1978) et dans l'absorption du phosphore (Shirazi, Beechey et Butterworth, 1981 ; Ulmann, 1982 ;Kempson et a/., 1979 ;Birge et Avioli, 19811. Chez le rat ou le poulet, l'activité de l'une et l'autre enzymes augmente avec l'apport de vitamine D tant in vivo (Steenbock et a/., 1953 ; Pileggi, De Luca et Steenbock, 1955 ; Davies, Ritcey et Motzok, 1970 ;Roberts et Yudkins, 1961 ;Bhandari, 1980 ;Valinietse et Bauman, 1984) qu'in vitro (Peterlik et Wasserman, 1980 ;Chan et Atkins, 1983 ; Haussler, Nagode et Rasmussen, 1970 ;Bikle et al, 1979 ; Lacour, 1979).…”

Section: Introductionunclassified

Absence d'effet de la vitamine D sur la phytase et la phosphatase alcaline intestinales : relation avec l'absorption du phosphore phytique chez le porc

Fontaine¹,

Fourdin²,

Pointillart³

et al. 1985

Reprod. Nutr. Dévelop.

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P absorption and retention was two times higher in + D pigs than in -D animals (balance technique), and tibia X-ray pictures showed a lower bone density with the -D diet than with the + D diet. Surprisingly, vit. D supplementation had no effect on either of the mucosal enzymes (phytase and alkaline phosphatase). It may be concluded that vit. D improves phytate-P absorption via a mechanism which does not involve an increase in the activity of the intestinal phosphatases.Introduction.

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Inorganic Phosphate Absorption in Small Intestine

Danisi¹,

Murer

1991

Comprehensive Physiology

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Alkaline phosphatase in adaptation to low dietary phosphate intake.

Cited by 39 publications

References 35 publications

Renal Adaptation to a Low Phosphate Diet in Rats

Renal Adaptation to a Low Phosphate Diet in Rats

Absence d'effet de la vitamine D sur la phytase et la phosphatase alcaline intestinales : relation avec l'absorption du phosphore phytique chez le porc

Inorganic Phosphate Absorption in Small Intestine

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