Alkaline phosphatase labeled SERS active sandwich immunoassay for detection of Escherichia coli

Bozkurt, Akif Göktuğ; Buyukgoz, Guluzar Gorkem; Soforoglu, Mehmet; Tamer, Uğur; Suludere, Zekiye; Boyaci, İsmail Hakkı

doi:10.1016/j.saa.2017.12.057

Cited by 39 publications

(21 citation statements)

References 45 publications

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“…The method was very sensitive, with a limit of detection of the order of 10 CFU mL −1 . References 19 to 23 of Bozkurt et al (2018) provide additional examples of using SERS to detect E. coli. Gao, Pearson, and He (2018) used the properties of 4-mercaptophenylboronic acid (4-MPBA) to construct a SERS sensor for bacteria.…”

Section: Sersmentioning

confidence: 99%

“…Copyright © 2017 Nature F I G U R E 8 An overview of ALP labeled SERS active assay for E. coli detection. Reprinted with permission from Bozkurt et al (2018). Copyright © 2018 Elsevier F I G U R E 9 SERS maps of surface of sensor for different concentrations of E. coli O157:H7.…”

Section: Sersmentioning

confidence: 99%

“…The method was very sensitive, with a limit of detection of the order of 10 CFU mL −1 . References 19 to 23 of Bozkurt et al () provide additional examples of using SERS to detect E. coli .…”

Section: Single Detection Of Foodborne Pathogensmentioning

confidence: 99%

Section: Single Detection Of Foodborne Pathogensmentioning

confidence: 99%

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Single and multiple detections of foodborne pathogens by gold nanoparticle assays

Pissuwan

Gazzana

Mongkolsuk

et al. 2019

WIREs Nanomed Nanobiotechnol

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A late detection of pathogenic microorganisms in food and drinking water has a high potential to cause adverse health impacts in those who have ingested the pathogens. For this reason there is intense interest in developing precise, rapid and sensitive assays that can detect multiple foodborne pathogens. Such assays would be valuable components in the campaign to minimize foodborne illness. Here, we discuss the emerging types of assays based on gold nanoparticles (GNPs) for rapidly diagnosing single or multiple foodborne pathogen infections. Colorimetric and lateral flow assays based on GNPs may be read by the human eye. Refractometric sensors based on a shift in the position of a plasmon resonance absorption peak can be read by the new generation of inexpensive optical spectrometers. Surface‐enhanced Raman spectroscopy and the quartz microbalance require slightly more sophisticated equipment but can be very sensitive. A wide range of electrochemical techniques are also under development. Given the range of options provided by GNPs, we confidently expect that some, or all, of these technologies will eventually enter routine use for detecting pathogens in food. This article is categorized under: Diagnostic Tools > Biosensing

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Section: Sersmentioning

confidence: 99%

Section: Sersmentioning

confidence: 99%

Section: Single Detection Of Foodborne Pathogensmentioning

confidence: 99%

Section: Single Detection Of Foodborne Pathogensmentioning

confidence: 99%

See 2 more Smart Citations

Single and multiple detections of foodborne pathogens by gold nanoparticle assays

Pissuwan

Gazzana

Mongkolsuk

et al. 2019

WIREs Nanomed Nanobiotechnol

View full text Add to dashboard Cite

show abstract

“…The formed Au@Ag nanostructure had strong SERS signal detection for AFP ranging from 0.5 to 100 pg/mL with LOD of 0.081 pg/mL (3σ). Bozkurt et al (2018) developed alternative methods using ALP activity for E. coli detection [76]. Three steps were using including modification of spherical magnetic gold coated core-shell nanoparticles and application of immunomagnetic separation to capture E. coli from solution.…”

Section: Optical Detection Techniquesmentioning

confidence: 99%

Overview of Optical and Electrochemical Alkaline Phosphatase (ALP) Biosensors: Recent Approaches in Cells Culture Techniques

Balbaied

Moore

2019

Biosensors

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Alkaline phosphatase (ALP), which catalyzes the dephosphorylation process of proteins, nucleic acids, and small molecules, can be found in a variety of tissues (intestine, liver, bone, kidney, and placenta) of almost all living organisms. This enzyme has been extensively used as a biomarker in enzyme immunoassays and molecular biology. ALP is also one of the most commonly assayed enzymes in routine clinical practice. Due to its close relation to a variety of pathological processes, ALP’s abnormal level is an important diagnostic biomarker of many human diseases, such as liver dysfunction, bone diseases, kidney acute injury, and cancer. Therefore, the development of convenient and reliable assay methods for monitoring ALP activity/level is extremely important and valuable, not only for clinical diagnoses but also in the area of biomedical research. This paper comprehensively reviews the strategies of optical and electrochemical detection of ALP and discusses the electrochemical techniques that have been addressed to make them suitable for ALP analysis in cell culture.

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Applications of Nanomaterials for Greener Food Analysis

Garg

Singh

Sharma

et al. 2022

Green Chemical Analysis and Sample Preparations

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Alkaline phosphatase labeled SERS active sandwich immunoassay for detection of Escherichia coli

Cited by 39 publications

References 45 publications

Single and multiple detections of foodborne pathogens by gold nanoparticle assays

Single and multiple detections of foodborne pathogens by gold nanoparticle assays

Overview of Optical and Electrochemical Alkaline Phosphatase (ALP) Biosensors: Recent Approaches in Cells Culture Techniques

Applications of Nanomaterials for Greener Food Analysis

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